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2.3.3.16: citrate synthase (unknown stereospecificity)

This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.

Word Map on EC 2.3.3.16

Reaction

acetyl-CoA
+
H2O
 +
oxaloacetate
=
citrate
 +
CoA

Synonyms

bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.16 citrate synthase (unknown stereospecificity)

Storage Stability

Storage Stability on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°C, 7-10 mg/ml, 50 mM Tris buffer, pH 8.0, stable for at least 1 year
-20°C, potassium phosphate buffer, pH 7.2, 15% glycerol, several weeks
-
-20°C, Tris-HCl buffer, pH 7.4, 50% glycerol, 1 month
-
-20°C, Tris-HCl buffer, pH 8.0, 1 year
-
0°C, below, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol and 5 mg/ml bovine serum albumin, several months
-
4°C, 20% glycerol, 0.2mM oxaloacetate, 6 months, isoenzyme CSII stable, isoenzyme CSI 45% loss of activity
-
4°C, 25 mM Tris-HCl, pH 7.7, loss of 80% activity within 3 days, 50 mM KCl protects
-
4°C, phosphate buffer, pH 7.4, 20% loss of activity after 2 weeks
-
4°C, pure citrate synthase is completely stable for at least 6 months
-
4°C, Tris-HCl buffer, pH 7.0, 1 mM dithiothreitol, 1 day
-
4°C, Tris-HCl buffer, pH 7.6, 0.2 M KCl, 20% loss of activity after 1 month, only CS II
-
4°C, Tris-HCl buffer, pH 7.6, high protein concentration, below 20% loss of activity in 2 months
-
4°C, Tris-HCl buffer, pH 8.0, 20% v/v glycerol and 0.1 M KCl, at least 6 months
-
4°C, Tris-HCl buffer, pH8.0, 50 mM glycerol, at least 1 week
-
room temperature, at 0.2 M KCl, the enzyme is stable at for at least 48 h