2.3.3.16: citrate synthase (unknown stereospecificity)

This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.

Reaction

Synonyms

bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.3 Acyl groups converted into alkyl groups on transfer

                2.3.3.16 citrate synthase (unknown stereospecificity)

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Results	in table
16	Activating Compound
21910	AA Sequence
9	Application
1	CAS Registry Number
52	Cloned(Commentary)
2	Cofactor
21	Crystallization (Commentary)
41	Disease/ Diagnostics
7	Expression
18	General Information
10	General Stability
238	Inhibitors
16	kcat/KM [mM/s]
77	Ki Value [mM]
209	KM Value [mM]
39	Localization
30	Metals/Ions
131	Molecular Weight [Da]
63	Natural Substrates/ Products (Substrates)
276	Organism
17	Pathway
64	PDB ID
42	pH Optimum
4	pH Range
2	pH Stability
19	pI Value
1	Posttranslational Modification
69	Protein Variants
62	Purification (Commentary)
15	Reaction
8	Reaction Type
205	Reference
1	Renatured (Commentary)
50	Source Tissue
72	Specific Activity [micromol/min/mg]
15	Storage Stability
193	Substrates and Products (Substrate)
85	Subunits
95	Synonyms
1	Systematic Name
56	Temperature Optimum [°C]
5	Temperature Range [°C]
30	Temperature Stability [°C]
96	Turnover Number [1/s]

Molecular Weight

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Molecular Weight on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)

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MOLECULAR WEIGHT

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

100000

8 entries

105000

2 entries

106000

Natronomonas pharaonis

-

gel filtration

799

110000

Dictyostelium discoideum

-

gel filtration

488034

111000

Halobacterium salinarum

-

gel filtration

799

112000

2 entries

150000

3 entries

171000

Staphylococcus aureus

A0A0H3K9Z0

multi-angle light scattering

736463

227000

Cupriavidus necator

-

gel filtration

488050

228000

Mesorhizobium ciceri

-

gel filtration

488078

240000

2 entries

242000

Acinetobacter calcoaceticus subsp. anitratus

-

sedimentation equilibrium centrifugation

488030

249000 - 275000

Azotobacter vinelandii

-

equilibrium sedimentation

488028

250000

Azotobacter vinelandii

-

sedimentation equilibrium centrifugation

488030

260000

Methylorubrum extorquens

-

gel filtration

488070

269000

Escherichia coli

-

gel filtration

488040

28000

Staphylococcus aureus

A0A0H3K9Z0

6 * 28000, SDS-PAGE

736463

280000

3 entries

287000

Nitrosomonas sp.

-

gel filtration

488071

300000

Pseudomonas sp.

-

CS II, gel filtration

488035

330000

Azotobacter vinelandii

-

gel filtration

488028

33200

Malus baccata var. xiaojinensis

-

x * 33200, calculated from amino acid sequence

737011

36500

Pseudomonas aeruginosa

-

2 * 36500, CS II, SDS-PAGE

488062

37500

Pseudomonas aeruginosa

-

isoenzyme CSI 6 * 37500, SDS-PAGE

488062

40000

Saccharolobus solfataricus

-

2 * 40000, SDS-PAGE

488048

40300

2 entries

41000

Sulfolobus acidocaldarius

-

2 * 41000, SDS-PAGE

488049

42000

4 entries

42600

Pyrococcus furiosus

-

2 * 42600, SDS-PAGE

488057

42679

Saccharolobus solfataricus

P80148

x * 42679, calculated from sequence

724945

43000

Thermoplasma acidophilum

-

2 * 43000, SDS-PAGE

488049

43290

Pyrococcus furiosus

Q53554

2 * 43290, calculated from amino acid sequence

719429

44000

Escherichia coli

-

6 * 44000, SDS-PAGE, 6 * 49000, guanidine-HCl gel filtration

488040

44700

Nitrosomonas sp.

-

6 * 44700, SDS-PAGE

488071

45000

2 entries

46000

Komagataeibacter europaeus

-

6 * 46000, SDS-PAGE, amino-terminal amino acid sequence

488051

47000

Cupriavidus necator

-

6 * 47000, SDS-PAGE, or homopentamer

488050

47885

Escherichia coli

P0ABH7

6 * 47885, calculated from amino acid sequence

735698

48000

4 entries

48700

Drosophila melanogaster

-

2 * 48700, SDS-PAGE

488059

49000

2 entries

494770

Puccinia striiformis f. sp. tritici

-

gel filtration

756609

49868

Streptomyces diastaticus

G8XQY5

2 * 49868, MALDI-TOF mass spectrometry

735799

50000

Saccharomyces cerevisiae

-

2 * 50000, CS II, SDS-PAGE

488068

51100

Clostridium kluyveri

-

x * 51100, calculated from sequence

674333

51700

Citrus junos

Q6T8H1

x * 51700, calculated

706384

52000

Fasciola hepatica

M4QLE7

2 * 52000, SDS-PAGE

736942

52200

Aspergillus nidulans

O00098

x * 52200, deduced from amino acid sequence

720215

53000

2 entries

58500

Azotobacter vinelandii

-

4 * 58500, sedimentation equilibrium in guanidine-HCl and dithiothreitol

488030

60000 - 70000

Talaromyces trachyspermus

-

gel filtration

488029

60000 - 95000

Bacillus subtilis

-

gel filtration, sedimentation equilibrium centrifugation

488030

66000

Methylorubrum extorquens

-

4 * 66000, SDS-PAGE

488070

68000

Thermoplasma acidophilum

-

gel filtration

488043

69000

Acinetobacter calcoaceticus subsp. anitratus

-

4 * 69000, sedimentation equilibrium in guanidine-HCl and dithiothreitol

488030

73000

Sulfolobus acidocaldarius

-

gel filtration

488043

78000

Cicer arietinum

-

gel filtration

488078

80000

2 entries

80300

2 entries

81000

Drosophila melanogaster

-

gel filtration

488059

83000

Sulfolobus acidocaldarius

-

gel filtration

799, 488049

84000

2 entries

85000

Thermoplasma acidophilum

-

gel filtration

799, 488049

88533

Nostoc sp. PCC 7120 = FACHB-418

Q9R6Y4

MALDI-TOF

758279

89700

Pyrococcus furiosus

-

gel filtration

488057

98000

Thermus aquaticus

-

gel filtration

488036

99990

Streptomyces diastaticus

G8XQY5

gel filtration

735799

additional information

36 entries

100000

Saccharomyces cerevisiae

-

gel filtration

488068

100000

Pseudomonas sp.

-

gel filtration

488035

100000

Rhodothermus marinus

-

gel filtration

488087

100000

Heliocidaris crassispina

-

gel filtration

488037

100000

Arabidopsis thaliana

P20115

gel filtration

736845

100000

Rhodothermus marinus

-

dimeric enzyme form

488087

100000

Pseudomonas sp.

-

CS I

488035

100000

Saccharomyces cerevisiae

-

CS II

488068

105000

Haloferax volcanii

-

gel filtration

799

105000

Natronococcus occultus

-

gel filtration

799

112000

Haloarcula vallismortis

-

gel filtration

799

112000

Natronobacterium gregoryi

-

gel filtration

799

150000

Arthrobacter sp.

-

above: gram-negative methylotrophs, below: gram-positive methylotrophs

488044

150000

Methylophilus methylotrophus

-

above: gram-negative methylotrophs, below: gram-positive methylotrophs

488044

150000

Hyphomicrobium sp.

-

above: gram-negative methylotrophs, below: gram-positive methylotrophs

488044

240000

Pseudomonas aeruginosa

-

gel filtration, CS I

488062

240000

Pseudomonas aeruginosa

-

native CSI, gel filtration

488062

280000

Rhodothermus marinus

-

gel filtration

488087

280000

Komagataeibacter europaeus

-

gel filtration

488051

280000

Rhodothermus marinus

-

hexameric enzyme form

488087

40300

Priestia megaterium

-

2 * 40300, SDS-PAGE, gel filtration in guanidine-HCl, amino acid composition

488041

40300

Priestia megaterium

-

2 * 40300, SDS-PAGE, gel filtration in guanidinium chloride

488041

42000

Haloferax volcanii

D4GS06

x * 42000, SDS-PAGE

727213

42000

Rhodothermus marinus

-

2 * 42000, dimeric enzyme form, SDS-PAGE

488087

42000

Pseudomonas aeruginosa

-

6 * 42000, CS i, SDS-PAGE

488062

42000

Pseudomonas aeruginosa

-

isoenzyme CSII 2 * 42000, SDS-PAGE

488062

45000

Haloferax volcanii

-

x * 45000, SDS-PAGE

488066

45000

Rhodothermus marinus

-

6 * 45000, hexameric enzyme form

488087

48000

Streptomyces diastaticus

G8XQY5

2 * 48000, SDS-PAGE

735799

48000

Azotobacter vinelandii

-

6 * 48000, SDS-PAGE

488028

48000

Aspergillus niger

Q9P8D6

2 * 48000, mitochondrial enzyme, SDS-PAGE

488074

48000

Citrullus lanatus subsp. vulgaris

-

x * 48000, glyoxysomal isozyme, SDS-PAGE

488047

49000

Tetrahymena pyriformis

-

x * 49000, SDS-PAGE

488063

49000

Escherichia coli

-

6 * 44000, SDS-PAGE, 6 * 49000, guanidine-HCl gel filtration

488040

53000

Pseudomonas sp.

-

2 * 53000, SDS-PAGE, CS I, reassociation to CS II possible

488035

53000

Pseudomonas sp.

-

6 * 53000, SDS-PAGE, CS II

488035

80000

Saccharolobus solfataricus

-

gel filtration

488048

80000

Aspergillus niger

Q9P8D6

gel filtration

488074

80300

Pseudomonas aeruginosa

-

CS II, gel filtration

488062

80300

Pseudomonas aeruginosa

-

native CSII, gel filtration

488062

84000

Priestia megaterium

-

gel filtration

488041

84000

Priestia megaterium

-

Stokes radius, sedimentation coefficient

488041

additional information

Drosophila melanogaster

-

amino acid composition

488059

additional information

Pseudomonas aeruginosa

-

amino acid composition

488062

additional information

Haloferax volcanii

-

amino acid sequence

488066

additional information

Aspergillus nidulans

Q9TEM3

amino acid sequence

488089

additional information

Pyrococcus furiosus

-

amino acid sequence alignment

488057

additional information

Escherichia coli

P0ABH7

amino acid sequence alignment

488085

additional information

Escherichia coli

-

amino acid sequence alignment

488085

additional information

Solanum tuberosum

-

amino acid sequence comparison

488054

additional information

Escherichia coli

-

alignment of partial amino acid sequence

488069

additional information

Acinetobacter calcoaceticus subsp. anitratus

-

alignment of partial amino acid sequence

488069

additional information

Azotobacter vinelandii

-

alignment of partial amino acid sequence

488028

additional information

Rhodothermus marinus

-

N-terminal protein sequence

488087

additional information

Komagataeibacter europaeus

-

N-terminal protein sequence

488051

additional information

Aspergillus niger

Q9P8D6

C-terminal amino acid sequence alignment

488074

additional information

Bacillus subtilis

-

small and large enzyme

488044

additional information

Arthrobacter sp.

-

small and large enzyme

488044

additional information

Methylophilus methylotrophus

-

small and large enzyme

488044

additional information

Hyphomicrobium sp.

-

small and large enzyme

488044

additional information

Saccharomyces cerevisiae

-

enzyme participates in multienzyme complexes of enzymes belonging to tricarbonic acid cycle, probably substrate channeling, in vivo NMR measurements

488072

additional information

Thermus aquaticus

-

gram-negative bacteria: approximately 250000, gram-positive-bacteria and eucaryotes: approximately 100000

488036

additional information

Pyrococcus furiosus

-

secondary and tertiary structure

488057

additional information

Pyrococcus furiosus

Q53554

secondary and tertiary structure

488045

additional information

Thermoplasma acidophilum

-

N-terminal protein sequence alignment

488049

additional information

Aspergillus niger

Q9P8D6

N-terminal protein sequence alignment

488074

additional information

Thermoplasma acidophilum

-

free enzyme forms various binary and ternary complexes with substrates

488082

additional information

Comamonas testosteroni

-

one isoenzyme, large form

662550

additional information

Delftia acidovorans

-

one isoenzyme, large form

662550

additional information

Brevundimonas diminuta

-

one isoenzyme, large form

662550

additional information

Pseudomonas alcaligenes

-

one isoenzyme, small form

662550

additional information

Pelomonas saccharophila

-

one isoenzyme, small form

662550

additional information

Pseudomonas chlororaphis

-

one isoenzyme, small form

662550

additional information

Stenotrophomonas maltophilia

-

one isoenzyme, small form

662550

additional information

Pseudomonas fluorescens

-

two isoenzymes, a large and a small form

662550

additional information

Pseudomonas aeruginosa

-

two isoenzymes, a large and a small form

662550

additional information

Pseudomonas putida

-

two isoenzymes, a large and a small form

662550

additional information

Pseudomonas stutzeri

-

two isoenzymes, a large and a small form

662550