2.3.3.16: citrate synthase (unknown stereospecificity)
This is an abbreviated version!
For detailed information about citrate synthase (unknown stereospecificity), go to the full flat file.
Reaction
+
+
=
+
Synonyms
bifunctional citrate synthase/2-methylcitrate synthase, CCNA_01983, CIT1, CitA, citrate condensing enzyme, citrate oxaloacetate-lyase (CoA-acetylating), citrate oxaloacetate-lyase, CoA-acetylating, citrate synthase, citrate synthase Cit1, citrate synthase/2-methylcitrate synthase, citrate synthetase, citric synthase, citric-condensing enzyme, citrogenase, CitZ, CS, CS1, CS2, CS3, CS4, CSI, CSY, CSY4, CTS, EC 4.1.3.7, gltA, GltA2, MCS, mitochondrial citrate synthase, mmgD, More, Msed_1522, oxalacetic transacetase, oxaloacetate transacetase, peroxisomal citrate synthase, Rv0896, SbnG, Si-citrate synthase, sll0401, SSO2589, St0589, St1805, synthase, citrate, TTHA1343, type II citrate synthase
ECTree
KI Value
KI Value on EC 2.3.3.16 - citrate synthase (unknown stereospecificity)
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0.003 - 0.051
oxaloacetate
0.0024 - 0.0225
propionyl-CoA
0.00037 - 0.0024
S-carboxymethyl-CoA
additional information
additional information
-
mutants, overview
-
0.3
2-oxoglutarate
-
below
0.38
2-oxoglutarate
-
versus oxaloacetate, competitive
0.76
2-oxoglutarate
-
wild-type
20
2-oxoglutarate
-
above
20
2-oxoglutarate
-
above
0.125
ATP
-
versus acetyl-CoA
0.33
ATP
-
mitochondrial isozyme
0.75
ATP
-
versus acetyl-CoA
1.9
ATP
30°C, pH 8, versus acetyl-CoA
2.6
ATP
-
glyoxysomal isozyme
4.34
ATP
substrate oxaloacetate, pH 8.0, 25°C
8.93
ATP
pH not specified in the publication, temperature not specified in the publication
2.4
citrate
pH not specified in the publication, temperature not specified in the publication
7.19
citrate
substrate oxaloacetate, pH 8.0, 25°C
0.0017
NADH
-
mutant enzyme R109L
0.0028
NADH
-
wild-type enzyme
0.012
NADH
-
in 20 mM Tris-HCl
0.018
NADH
-
mutant enzyme Q182A
0.08
NADH
-
mutant enzyme T111A
0.121
NADH
-
mutant enzyme H110A
0.165
NADH
-
mutant enzyme T204A
0.242
NADH
-
mutant enzyme N189A
0.4
NADH
-
mutant enzyme R163L
0.4 - 12
NADH
-
strains X and Hanham
0.63
NADH
-
mutant enzyme K167A
0.66
NADH
-
in 0.1 M Tris-HCl
0.79
NADH
-
mutant enzyme Y145A
4.41
NADH
substrate oxaloacetate, pH 8.0, 25°C
7.96
NADH
substrate acetyl-CoA, pH 8.0, 25°C
0.8
NADPH
-
-
0.003
oxaloacetate
-
mutant enzyme H110A, in presence of 0.1 M KCl
0.004
oxaloacetate
-
mutant enzyme T204A, in presence of 0.1 M KCl
0.005
oxaloacetate
-
mutant enzyme R163L, in presence of 0.1 M KCl
0.007
oxaloacetate
-
mutant enzyme R109L, in presence of 0.1 M KCl
0.01
oxaloacetate
-
mutant enzyme Q182A, in presence of 0.1 M KCl
0.017
oxaloacetate
-
mutant enzyme N189A, in presence of 0.1 M KCl
0.018
oxaloacetate
-
mutant enzyme T111A, in presence of 0.1 M KCl
0.026
oxaloacetate
-
wild-type enzyme, in presence of 0.1 M KCl
0.037
oxaloacetate
-
mutant enzyme K167A, in presence of 0.1 M KCl
0.051
oxaloacetate
-
mutant enzyme Y145A, in presence of 0.1 M KCl
0.0024
propionyl-CoA
-
versus acetyl-CoA, competitive
0.0225
propionyl-CoA
-
versus oxaloacetate, noncompetitive
0.00037
S-carboxymethyl-CoA
25°C, pH and temperature not specified in the publication, competitive inhibition versus acetyl-CoA
0.00037
S-carboxymethyl-CoA
pH and temperature not specified in the publication, competitively inhibition versus acetyl-CoA
0.0024
S-carboxymethyl-CoA
25°C, pH and temperature not specified in the publication, non-competitive inhibition versus oxaloacetate
0.0024
S-carboxymethyl-CoA
non-competitively inhibition versus oxaloacetate, pH and temperature not specified in the publication