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2.3.3.10: hydroxymethylglutaryl-CoA synthase

This is an abbreviated version!
For detailed information about hydroxymethylglutaryl-CoA synthase, go to the full flat file.

Word Map on EC 2.3.3.10

Reaction

acetyl-CoA
+
H2O
 +
acetoacetyl-CoA
=
(S)-3-hydroxy-3-methylglutaryl-CoA
 +
CoA

Synonyms

(S)-3-hydroxy-3-methylglutaryl-CoA acetoacetyl-CoA-lyase (CoA-acetylating), 3-hydroxy-3-methylglutaryl (HMG)-CoA synthase, 3-hydroxy-3-methylglutaryl CoA synthase, 3-hydroxy-3-methylglutaryl CoA synthase I, 3-hydroxy-3-methylglutaryl CoA synthetase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthetase, 3-hydroxy-3-methylglutaryl-CoA synthase, 3-hydroxy-3-methylglutaryl-CoA synthase 1, 3-hydroxy-3-methylglutaryl-CoA synthase 2, 3-hydroxy-3-methylglutaryl-coenzyme A synthase, 3-hydroxy-3-methylglutaryl_coenzyme A synthase, 3-hydroxyl-3-methyl-glutaryl-CoA synthase, acetoacetyl coenzyme A transacetase, beta-hydroxy-beta-methylglutaryl-CoA synthase, BjHMGS1, BjHMGS2, BjHMGS3, BjHMGS4, EC 4.1.3.5, GbHMGS2, GhHMGS1A, GhHMGS1D, GhHMGS2D, GhHMGS3A, GhHMGS3D, HGMS, HMG-CoA, HMG-CoA synthase, HMG-CoA synthase 1, HMG-CoS synthase, HMGCS, HMGCS1, HMGCS2, HMGS, HMGS-2, HMGS1, HMGS2, hydroxy-methylglutaryl coenzyme-A synthase, hydroxymethylglutaryl CoA synthase 2, hydroxymethylglutaryl CoA synthetase, hydroxymethylglutaryl coenzyme A synthase, hydroxymethylglutaryl coenzyme A-condensing enzyme, hydroxymethylglutaryl-CoA synthase, hydroxymethylglutaryl-coenzyme A synthase, LcHMGS, mvaS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.10 hydroxymethylglutaryl-CoA synthase

Storage Stability

Storage Stability on EC 2.3.3.10 - hydroxymethylglutaryl-CoA synthase

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-20°, stored in presence of dithiothreitol and 30-50% glycerol purified enzyme retains complete activity for up to 3 months
-
-20°C, may be stored in 30% glycerin solution for 2-3 months with no loss of activity
-
-20°C, purified synthase may be stored for 2-3 months with little loss of activity by adding 30% glyerol
-
-20°C, stored in presence of 30% glycerol and 5 mM dithiothreitol, remains active for several months
-
-80°C, recombinant enzyme, negligible loss of activity after 1 year
-
-85°C, in presence of 30% glycerol stable for over 1 year
-
-90°C, purified synthases II-IV in 20 mM potassium phosphate, pH 7.0, 5 mM dithiothreitol, can be stored as pellets following precipitation by 60% saturated ammonium sulfate up to 3 months without loss of activity
-
0°C, purified synthase I stored frozen in 40% glycerol containing 20 mM potassium phosphate, pH 7.0, 5 mM dithiothreitol, stable for 3-4 months
-
4°C, enzyme loses activity in absence of glycerol
-
4°C, in presence of 30% glycerol stable for several days
-
4°C, purified synthase I stored in 40% glycerol containing 20 mM potassium phosphate, pH 7.0, 5 mM dithiothreitol is unstable
-
4°C, purified synthases II-IV in 20 mM potassium phosphate, pH 7.0, 5 mM dithiothreitol, stable for several weeks
-
4°C, recombinant enzyme, loses 50% activity after 1 year
-