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2.3.3.1: citrate (Si)-synthase

This is an abbreviated version!
For detailed information about citrate (Si)-synthase, go to the full flat file.

Word Map on EC 2.3.3.1

Reaction

acetyl-CoA
+
H2O
 +
oxaloacetate
=
citrate
 +
CoA

Synonyms

(R)-citric synthase, citrate oxaloacetate-lyase ((pro-3S)-CH2COO---> acetyl-CoA), citrate synthase, citrate synthase isoform a, citrate synthase isoform b, CLO1313_RS02945, CS, EC 4.1.3.7, HCS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.3 Acyl groups converted into alkyl groups on transfer
                2.3.3.1 citrate (Si)-synthase

Renatured

Renatured on EC 2.3.3.1 - citrate (Si)-synthase

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RENATURED/Commentary
ORGANISM
UNIPROT
LITERATURE
only after removal of 6 M denaturing guanidinium chloride by dialysis, not by dilution, dithiothreitol-dependent
-
study on the mechanism of aggregation during refolding of enzyme and its prevention using cosolvent additives of the polyol series. No parallel correlation between the folding effect and the general stabilization is observed. Glycerol is most effective in enhancing the refolding yield of citrate synthase, and a complete recovery of enzymatic activity is observed at 7 M glycerol and 0.01 mg per ml protein. Kinetic experiments suggest that polyols act very early in the refolding process. Both the thermodynamic and the kinetic aspects are critical in the folding process
-