2.3.2.B14: L,D-transpeptidase
This is an abbreviated version!
For detailed information about L,D-transpeptidase, go to the full flat file.
Word Map on EC 2.3.2.B14
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2.3.2.B14
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tuberculosis
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mycobacterium
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carbapenems
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penicillin-binding
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d,d-transpeptidases
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enterococcus
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faecium
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ldtmt2
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transpeptidases
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imipenem
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ertapenem
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muropeptides
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acylenzyme
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clavulanate
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transpeptidation
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d-ala
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lineage-determining
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doripenem
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d,d-carboxypeptidase
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analysis
- 2.3.2.B14
- tuberculosis
- mycobacterium
- carbapenems
-
penicillin-binding
-
d,d-transpeptidases
- enterococcus
- faecium
- ldtmt2
- transpeptidases
- imipenem
- ertapenem
- muropeptides
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acylenzyme
- clavulanate
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transpeptidation
- d-ala
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lineage-determining
- doripenem
- d,d-carboxypeptidase
- analysis
Reaction
Generates 3->3 cross-links in peptidoglycan, catalyzing the cleavage of the mDap(3)-D-Ala4 bond of a tetrapeptide donor stem and the formation of a bond between the carbonyl of mDap3 of the donor stem and the side chain of mDap3 of the acceptor stem. =
Synonyms
CLIBASIA_01175, IprQ, L,D-transpeptidase, L,D-transpeptidase 2, L,D-transpeptidase 5, LdtB, LdtBS, LdtF, Ldtfm, Ldtfm217, Ldtfs, LdtMt1, LdtMt2, LdtP, MAB_1530, MAB_3165c, MT0125, MT0501, MT2594, Rv1433, Rv2518c, transpeptidase, YcbB
ECTree
2.3.2.B14 L,D-transpeptidaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.3.2.B14 - L,D-transpeptidase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
2 GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala 3->3 crosslinked at the mesoDapNH2 position
2 Gln-N-acetyl-Mur-L-Ala-D-Glu-diaminopimelyl-D-Ala
(Gln-N-acetyl-Mur-L-Ala-D-Glu-diaminopimelyl)2 + 2 D-Ala
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?
nitrocefin + H2O
(2R)-2-[(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl]-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
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?
peptidyl-tRNA1 + alpha-aminoacyl-tRNA2
tRNA1 + peptidyl-amino-tRNA2
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?
2 GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala 3->3 crosslinked at the mesoDapNH2 position
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?
2 GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala
GlcNAc-MurNAc-L-Ala-D-iGluNH2-meso-diaminopimelic acid-NH2-D-Ala 3->3 crosslinked at the mesoDapNH2 position
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?
additional information
?
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the enzyme is specific for disaccharide-peptides containing an L-Ala-L-Ala side chain in both the acceptor and donor substrates
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?
additional information
?
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the enzyme is specific for disaccharide-peptides containing an L-Ala-L-Ala side chain in both the acceptor and donor substrates
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?
additional information
?
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isoforms Ltd2 and Ltd3 display both L,D-transpeptidase (LDT) and L,D-carboxypeptidase activities
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additional information
?
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the primary sequence of the stem peptide can control Ldt cross-linking levels
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additional information
?
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incubation of YcbB with a disaccharide-tetrapeptide prepared from the peptidoglycan of Escherichia coli results in the formation of a peptidoglycan dimer containing a diaminopimelate3 -diaminopimelate3 cross-link. Purified YcbB uses as the acyl donor a disaccharide-tetrapeptide ending in D-Ala4, but not a disaccharide-pentapeptide ending in D-Ala4-D-Ala5
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additional information
?
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gene MT2594 (Rv2518c) encodes an L,Dtranspeptidase for synthesis of non-classical 3->3 cross-linkages
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?
additional information
?
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the enzyme also shows low beta-lactamase activity with nitrocefin (i.e. 3-(2,4-dinitrostyryl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid) with Km of 0.016 mM and kcat of 0.01 s-1 (in 20 mM HEPES (pH 7.5) and 300 mM NaCl, temperature not specified in the publication)
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?
additional information
?
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the enzyme also shows low beta-lactamase activity with nitrocefin (i.e. 3-(2,4-dinitrostyryl)-(6R,7R)-7-(2-thienylacetamido)-ceph-3-em-4-carboxylic acid) with Km of 0.016 mM and kcat of 0.01 s-1 (in 20 mM HEPES (pH 7.5) and 300 mM NaCl, temperature not specified in the publication)
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?
additional information
?
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gene MT2594 (Rv2518c) encodes an L,Dtranspeptidase for synthesis of non-classical 3->3 cross-linkages
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?
