2.3.2.B1: lipid II:alanine alanyl transferase

This is an abbreviated version!
For detailed information about lipid II:alanine alanyl transferase, go to the full flat file.

Reaction

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.2 Aminoacyltransferases

                2.3.2.B1 lipid II:alanine alanyl transferase

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Results	in table
2	AA Sequence
1	Cloned(Commentary)
8	General Information
2	IC50 Value
2	Inhibitors
5	kcat/KM [mM/s]
5	KM Value [mM]
12	Organism
1	Purification (Commentary)
1	Reaction
8	Reference
8	Specific Activity [micromol/min/mg]
20	Substrates and Products (Substrate)
1	Systematic Name
5	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.3.2.B1 - lipid II:alanine alanyl transferase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

ditrans,ocatacis-undecaprenyl diphosphoryl (GlcNAc-beta-(1->4))MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-Ala-tRNa

ditrans,ocatacis-undecaprenyl diphosphoryl (GlcNAc-beta-(1->4))MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae

-

-

711438

-

-

?

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

? + tRNA

3 entries

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA

? + tRNA

3 entries

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA

4 entries

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA

2 entries

additional information

?

-

7 entries

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

? + tRNA

Streptococcus pneumoniae

-

i.e. lipid I

712366

-

-

?

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

? + tRNA

Streptococcus pneumoniae 159

-

i.e. lipid I

712366

-

-

?

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

? + tRNA

Streptococcus pneumoniae Pn16

-

i.e. lipid I

712366

-

-

?

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA

? + tRNA

Streptococcus pneumoniae

-

-

712366

-

-

?

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA

? + tRNA

Streptococcus pneumoniae 159

-

-

712366

-

-

?

ditrans,ocatacis-undecaprenyl diphosphoryl MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanyl-L-alanyl-gamma-D-glutamyl-L-lysine + alanyl-tRNA

? + tRNA

Streptococcus pneumoniae Pn16

-

-

712366

-

-

?

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae

-

in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation

712366

-

-

?

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae

-

MurMPn16 of penicillin-susceptible strain Pn16 supports serylation more than alanylation both in vivo and in vitro

712366

-

-

?

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae 159

-

in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation

712366

-

-

?

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-alanyl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-alanyl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae Pn16

-

in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation

712366

-

-

?

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae

-

in vivo and in vitro, in the presence of the appropriate acyl-tRNA, MurM alanylates the peptidoglycan-amino group of the stem peptide lysine in preference to its serylation

712366

-

-

?

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl-D-alanyl-D-alanine + L-seryl-tRNA

UDP-MurNAc-L-alanyl-gamma-D-glutamyl-L-lysyl(N6-L-seryl)-D-alanyl-D-alanine + tRNA

Streptococcus pneumoniae

-

MurMPn16 of penicillin-susceptible strain Pn16 supports serylation more than alanylation both in vivo and in vitro

712366

-

-

?

additional information

?

-

Streptococcus pneumoniae

Q9L447

a 30-amino acid long sequence within the MurM protein is the main determinant of the specificity of the reaction, the addition of alanine versus serine

712350

-

-

?

additional information

?

-

Streptococcus pneumoniae

-

differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla

712366

-

-

?

additional information

?

-

Streptococcus pneumoniae

-

MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla

712366

-

-

?

additional information

?

-

Streptococcus pneumoniae 159

-

differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla

712366

-

-

?

additional information

?

-

Streptococcus pneumoniae 159

-

MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla

712366

-

-

?

additional information

?

-

Streptococcus pneumoniae Pn16

-

differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla

712366

-

-

?

additional information

?

-

Streptococcus pneumoniae Pn16

-

MurM acts during the lipid-linked stages of peptidoglycan synthesis.The D-alanyl-D-alanine of the stem peptide and the lipid II N-acetylglucosaminyl group are not essential for substrate recognition. epsilon-Carboxylation of the lysine of the stem peptide is not tolerated. Differing stem peptide structures of penicillin-resistant strain 159 and penicillin-susceptible strain Pn16 are caused by the profoundly greater catalytic efficiency of MurM159 compared with MurMPn16 brought about by sequence variation between these enzymes and, to a lesser extent, differences in the in vivo tRNAAla:tRNASer ratio in strains 159 and Pn16. Enzyme MurM additionally recognizes the acceptor stem and/or the TPSIC loop stem of the tRNAAla

712366

-

-

?