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2.3.2.27: RING-type E3 ubiquitin transferase

This is an abbreviated version!
For detailed information about RING-type E3 ubiquitin transferase, go to the full flat file.

Word Map on EC 2.3.2.27

Reaction

[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[E2 ubiquitin-conjugating enzyme]-L-cysteine
 +
[acceptor protein]-N6-ubiquitinyl-L-lysine

Synonyms

ABA-insensitive RING protein3, abscisic acid-insensitive RING protein 4, ACRE276, AIR1, AIR2, AIRP1, AIRP2, AIRP3, AIRP4, AMFR, Arabidopsis ABA-insensitive RING protein 1, Arabidopsis ABA-insensitive RING protein 2, ARIH1, As-induced RING E3 ligase 2, At1g54150, At1g74410, At2g15580, At4g23450, At4g26400, ATL2, ATL80, Avr9/Cf-9 rapidly elicited protein 276, BIG BROTHER, BIRC3, BRCA1, BRE1A, BRE1B, breast cancer susceptibility protein 1, breast cancer type 1 susceptibility protein, c-Cbl, C3HC4-RING finger E3 ubiquitin ligase, CaRING1, CHIP, cIAP2, Cop1, COP1 SUPPRESSOR1, CSU1, CUL4, cullin-4, DAF, DEAR1, DEFECTIVE IN ANTHER DEHISCENCE1 (DAD1)-activating factor, Der3, Dir1, E3 ligase, E3 ubiquitin ligase, E3 ubiquitin ligase Erysiphe necator-induced RING finger protein 1, E3 ubiquitin ligase RING1, E3 ubiquitin-protein ligase Arkadia, E3 ubiquitin-protein ligase Mdm2, E3 ubiquitin-protein ligase MIR1, E3 ubiquitin-protein ligase RNF25, E3-enzyme, EIRP1, EMR, ERAD-associated E3 ubiquitin-protein ligase DOA10, ERAD-associated E3 ubiquitin-protein ligase HRD1, ERAD-mediating RING finger protein, FANCL, gamma rays-induced RING finger protein1, GIRP1, gp78, H2-10, HCI1, HHARI, HIR1, HOS1, HSPC238, HTAS, HUB1, Human Homologue of Drosophila Ariadne, IDF1, IRT1 degradation factor1, KEEP ON GOING, KEG, Kf-1, LOG2, mahogunin ring finger-1, MARCH10, MARCH6, Mdm2, MEX3C, MGRN1, microtubule-associated E3 ubiquitin ligase isoform 1, MIEL1, MUL1, NERF, NFYA5 enhancing RING FINGER, Os05g41795, Os10g30850, Parkin, Parkinson juvenile disease protein 2, PIR1, PIR2, Pirh2, PLANT U-BOX17, PP2CA interacting RING finger protein 1, PP2CA interacting RING finger protein 2, pre-mRNA-splicing factor 19, PRP19, PUB17, PUB54, RanBP-type and C3HC4-type zinc finger-containing protein 1, RBCK1, RBX1, RCHY1, RFP1, RFPH2-10, RGLG2, RHA2b, Rines, Ring, RING E3, RING E3 ubiquitin ligase, ring finger protein 103, Ring finger protein 186, RING finger ubiquitin E3 ligase, RING-CH 10, ring-finer protein 55, RING-finger E3 ligase, RING-finger E3 ubiquitin ligase, RING-finger ubiquitin ligase Ubr1, RING-H2 finger E3 ubiquitin ligase, RING-H2 finger protein ATL63, RING-IBR-RING ubiquitin ligase, RING-type E3 ligase, RING-type E3 ubiquitin ligase, Ring1B, RMA1, RMA2, RMA3, RN181, RNF103, RNF111, RNF180, RNF186, RNF190, RNF2, RNF20, RNF220, RNF4, RNF43, RNF45, RNF8, salt tolerance RING finger 1, SDIR1, SIRP2, Sis3, SSM4, STIP1 homology and U-Box containing protein 1, Strf1, TEB4, TRIM22, Trim23, TRIM25, TRIM3, TRIM5, TRIM5alpha, TRIM62, TRIM69, tripartite motif-containing protein 5, U-box domain-containing protein 17, Ube4A, UBE4B, ubiquitin ligase E3, ubiquitin ligase Ubr1, ubiquitin-protein ligase K3, UBR1, UFD-2, Xbat32, ZNRF1

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.27 RING-type E3 ubiquitin transferase

Substrates Products

Substrates Products on EC 2.3.2.27 - RING-type E3 ubiquitin transferase

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SUBSTRATE
PRODUCT                       
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate) hide
LITERATURE
(Substrate)
COMMENTARY
(Product) hide
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2 [gp78-ubiquitin-carrier protein Ub2g2]-S-ubiquitinyl-L-cysteine
[gp78-ubiquitin-carrier protein Ub2g2]-S-[ubiquitinyl-N6-ubiquitinyl-L-lysine46]-L-cysteine
show the reaction diagram
use of mouse Ube2g2 ubiquitin conjugating enzyme. Ube2g2/gp78-mediated polyubiquitination involves preassembly of Lys 48-linked ubiquitin chains at the catalytic cysteine residue C89 of Ube2g2. The growth of Ube2g2-anchored ubiquitin chains seems to be mediated by an aminolysis-based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in its active site. Polyubiquitination of a substrate can be achieved by transferring preassembled ubiquitin chains from Ube2g2 to a lysine residue in a substrate
-
-
?
S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [POX1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[POX1]-L-lysine
show the reaction diagram
PIX1 i.e. peroxidase
contrary to substrates PGLU1, bHLH065 and GRP1, POX1 is polyubiquitylated
-
?
S-(ubiquitin)n-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [RNF186]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-(ubiquitin)n-[RNF186]-L-lysine
show the reaction diagram
BNip1 is a Bcl-2 family protein
isoform RNF186 undergoes RING-dependent self-ubiquitination
-
?
S-(ubiquitin)n-[Ubc10]-L-cysteine + [maltose-binding protein]-L-lysine
[Ubc10]-L-cysteine + N6-(ubiquitin)-[maltose-binding protein]-L-lysine
show the reaction diagram
-
-
polyubiquitylation of maltose-binding protein
-
?
S-(ubiquitin)n-[Ubc10]-L-cysteine + [RbcL1]-L-lysine
[Ubc10]-L-cysteine + N6-(ubiquitin)-[RbcL1]-L-lysine
show the reaction diagram
-
-
RbcL1 i.e. a Rubisco subunit
-
?
S-(ubiquitin)n-[Ubc10]-L-cysteine + [RbcS1]-L-lysine
[Ubc10]-L-cysteine + N6-(ubiquitin)-[RbcS1]-L-lysine
show the reaction diagram
-
-
RbcS1 i.e. a Rubisco subunit
-
?
S-(ubiquitin)n-[Ubc13]-L-cysteine + [ubiquitin]-L-lysine
[Ubc13]-L-cysteine + N6-(ubiquitin)n-[ubiquitin]-L-lysine
show the reaction diagram
-
-
human T lymphotropic virus type 1 trans-activator/oncoprotein Tax greatly stimulates RNF8 and Ubc13:Uev1A/Uev2 to assemble long K63-polyubiquitin chains
-
?
S-(ubiquitin)n-[UbcH5]-L-cysteine + [STRF1]-L-lysine
[UbcH5]-L-cysteine + N6-(ubiquitin)n-[STRF1]-L-lysine
show the reaction diagram
-
autoubiquitylation reaction leading to polyubiquitylated protein
-
?
S-ubiquinyl-[UbcH13]-L-cysteine + [acceptor protein]-L-lysine
[UbcH13]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme Ubc13]-L-cysteine + [acceptor protein]-L-lysine
[E2 ubiquitin-conjugating enzyme Ubc13]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme UbcH7]-L-cysteine + [acceptor protein]-L-lysine
[E2 ubiquitin-conjugating enzyme UbcH7]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ABI5]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ABI5]-L-lysine
show the reaction diagram
ABI5 i.e. abscisic acid-responsive transcription factor
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ACS4]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ACS4]-L-lysine
show the reaction diagram
ACS4 i.e. aminocyclopropane-1-carboxylic acid synthase 4
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [ACS7]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[ACS7]-L-lysine
show the reaction diagram
ACS4 i.e. aminocyclopropane-1-carboxylic acid synthase 7
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [bHLH065]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[bHLH065]-L-lysine
show the reaction diagram
bHLH065 i.e. ethylene-responsive protein
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [BNip1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[BNip1]-L-lysine
show the reaction diagram
BNip1 is a Bcl-2 family protein
BNip1 is polyubiquitinated by isoform RNF186 through K29 and K63 linkage in vivo. This modification promotes BNip1 transportation to mitochondria but has no influence on its protein level
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [GRP1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[GRP1]-L-lysine
show the reaction diagram
GRP1 i.e. glycine-rich cell-wall structural protein
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HCI1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[HCI1]-L-lysine
show the reaction diagram
-
autoubiquitylation reaction
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [histone H2A]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[histone H2A]-L-lysine
show the reaction diagram
-
E3-ligase activity of isoform Ring1b on histone H2A is enhanced by polycomb group protein Bmi1 in vitro. The N-terminal Ring-domains are sufficient for this activity and Ring1a can replace Ring1b. E2 enzymes UbcH5a, b, c or UbcH6 support this activity with varying processivity and selectivity
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [PGLU1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[PGLU1]-L-lysine
show the reaction diagram
PGLU12 i.e periplasmic beta-glucosidase. Isoform HCI1 accepts yeast E1 activating enzyme, and Arabidopsis thaliana E2 conjugating enzymes UBC10 and UBC11
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [Ring1b]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[Ring1b]-L-lysine
show the reaction diagram
-
autoubiquitination reaction, E2 enzymes UbcH5a, b, c or UbcH6 support autoubiquitination
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [SDIRIP1 protein]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[SDIRIP1 protein]-L-lysine
show the reaction diagram
-
-
-
-
?
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [TIP4.1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[TIP4.1]-L-lysine
show the reaction diagram
TIP4 i.e. tonoplast intrinsic protein 4
HIR1 strongly degrads the protein level of TIP4.1 via the ubiquitin 26S proteasome system
-
?
S-ubiquitinyl-[Hip2]-L-cysteine + [acceptor protein]-L-lysine
[Hip2]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
isoform RNF2 interacts with E2 protein Hip2, i.e. Huntingtin-interacting protein-2, and with Ubc4, UbcH5. RNF2 shows ubiquitin transferase E3 activity in the presence of Hip2
-
?
S-ubiquitinyl-[Ubc10]-L-cysteine + [GIRP1]-L-lysine
[Ubc10]-L-cysteine + N6-ubiquitinyl-[GIRP1]-L-lysine
show the reaction diagram
-
-
autoubiquitylation reaction
-
?
S-ubiquitinyl-[Ubc7]-L-cysteine + [CYP3A4]-L-lysine
[Ubc7]-L-cysteine + N6-ubiquitinyl-[CYP3A4]-L-lysine
show the reaction diagram
-
human liver endoplasmic reticulum-anchored cytochrome P450 enzyme CYP3A4 is degradedvia ubiquitylation by E2 ubiquitin-conjugating enzyme Ubc7/E3 ubiquitin-ligase gp78. CYP3A4 Asp/Glu/Ser(P)/Thr(P) surface clusters are important for its intermolecular electrostatic interactions with each of these E2-E3 subcomponents. By imparting additional negative charge to these Asp/Glu clusters, such Ser/Thr phosphorylation would generate P450 phosphodegrons for molecular recognition by the E2-E3 complexes, thereby controlling the timing of CYP3A4 ubiquitination and endoplasmic reticulum-associated degradation
-
?
S-ubiquitinyl-[Ubc8]-L-cysteine + [acceptor protein]-L-lysine
[Ubc8]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
the RING domain of Sis3 is sufficient for E3 ligase activity
-
?
S-ubiquitinyl-[UbcH5a]-L-cysteine + [p21]-L-lysine
[UbcH5a]-L-cysteine + N6-ubiquitinyl-[p21]-L-lysine
show the reaction diagram
-
substrate p21 is a protein necessary for the proliferation of a subset of platelet-derived growth factor-transformed proneural glioma cells
UbcH5a is a preferred E2 enzyme for TRIM3-dependent p21 ubiquitination. Ubiquitination is practically eliminated in a p21 K15R/K74R/K91R/K136R quadruple mutant
-
?
S-ubiquitinyl-[UbcH5a]-L-cysteine + [p53]-L-lysine
[UbcH5a]-L-cysteine + N6-ubiquitinyl-[p53]-L-lysine
show the reaction diagram
-
-
-
?
S-ubiquitinyl-[UbcH5b]-L-cysteine + [DAF]-L-lysine
[UbcH5b]-L-cysteine + N6-ubiquitinyl-[DAF]-L-lysine
show the reaction diagram
-
-
autoubiquitylation reaction
-
?
S-ubiquitinyl-[UbcH5b]-L-cysteine + [NERF]-L-lysine
[UbcH5b]-L-cysteine + N6-ubiquitinyl-[NERF]-L-lysine
show the reaction diagram
-
autoubiquitylation reaction
-
?
S-ubiquitinyl-[UbcH5c]-L-cysteine + [ERF53]-L-lysine
[UbcH5c]-L-cysteine + N6-ubiquitinyl-[ERF53]-L-lysine
show the reaction diagram
ERF53 i.e. ethylene response factor 53
RING E3 ligase RGLG2 mediates ERF53 ubiquitination for proteasomal degradation
-
?
S-ubiquitinyl-[UbcH5]-L-cysteine + [TRIM62]-L-lysine
[UbsH5B]-L-cysteine + N6-ubiquitinyl-[TRIM62]-L-lysine
show the reaction diagram
-
TRIM62, in association with the E2 enzyme UbcH5B, catalyzes self-ubiquitination in vitro. The process requires an intact RING finger domain. The treatment of HEK-293T cells with a proteasome inhibitor stabilizes poly-ubiquitinated TRIM62, indicating that self-ubiquitination promotes the proteasomal degradation of TRIM62
-
?
S-ubiquitinyl-[UbcM2]-L-cysteine + [acceptor protein]-L-lysine
[UbcM2]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine
show the reaction diagram
-
-
-
?
S-ubiquitinyl-[Ube2B]-L-cysteine + [March10a]-L-lysine
[Ube2B]-L-cysteine + N6-ubiquitinyl-[March10a]-L-lysine
show the reaction diagram
-
-
-
?
[ACRE276-RING domain-E3-ubiquitin-carrier protein HubC5B]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine
[ACRE276-RING domain-E3-ubiquitin-carrier protein]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
[BRCK1-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [BRCK1]-L-lysine
[BRCK1-ubiquitin-carrier protein]-L-cysteine + [BRCK1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
the RING1 finger plays an important role in the self-ubiquitination of RBCK1, leading to mono- and slightly diubiquitinated products. Self-ubiquitinated RBCK1 is processed by the proteasomal degradation
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-
?
[BRE1B-ubiquitin-carrier protein UbcH8]-S-ubiquitinyl-L-cysteine + [syntaxin1]-L-lysine
[BRE1B-ubiquitin-carrier protein UbcH8]-L-cysteine + [syntaxin1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
isoform BRE1B binds and recruits the brain-enriched E2 ubiquitin-conjugating enzyme UbcH8 to syntaxin 1 and facilitates the ubiquitination and proteasome-dependent degradation of syntaxin1
-
-
?
[c-Cbl-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [epidermal growth factor receptor]-L-lysine
[c-Cbl-ubiquitin-carrier protein]-L-cysteine + [epidermal growth factor receptor]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[CDC34]-S-ubiquitinyl-L-cysteine + [SCF]-L-lysine
[CDC34]-L-cysteine + [SCF]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
acceptor protein SCF consists of the cullin Cul1, the RING subunit Rbx1/Roc1/Hrt1, the adaptor protein Skp1, and an F-box protein such as Skp2 or TrCP that binds substrates
the I44A mutation in ubiquitin profoundly inhibits its ability to serve as a donor for ubiquitin chain initiation or elongation, but can be rescued by compensatory mutations in the E2 Cdc34
-
?
[E2 ubiquitin-conjugating enzyme Ub2k/Ube2w]-S-ubiquitinyl-L-cysteine + [BRCA1]-L-lysine
[E2 ubiquitin-conjugating enzyme Ube2k/Ube2w]-L-cysteine + [BRCA1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[E2 ubiquitin-conjugating enzyme Ubc10]-S-ubiquitinyl-L-cysteine + [AIR1]-L-lysine
[E2 ubiquitin-conjugating enzyme Ubc10]-L-cysteine + [AIR1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
-
?
[E2 ubiquitin-conjugating enzyme UBC10]-S-ubiquitinyl-L-cysteine + [SIRP2]-L-lysine
[E2 ubiquitin-conjugating enzyme UBC10]-L-cysteine + [SIRP2]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
-
?
[E2 ubiquitin-conjugating enzyme UBC10]-S-ubiquitinyl-L-cysteine + [TKL1]-L-lysine
[E2 ubiquitin-conjugating enzyme UBC10]-L-cysteine + [TKL1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
-
?
[E2 ubiquitin-conjugating enzyme Ubc13/Ube2w]-S-ubiquitinyl-L-cysteine + [BRCA1]-L-lysine
[E2 ubiquitin-conjugating enzyme Ubc13/Ube2w]-L-cysteine + [BRCA1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[E2 ubiquitin-conjugating enzyme UbcH5b]-S-ubiquitinyl-L-cysteine + [Ring finger domain of MEX3C]-L-lysine
[E2 ubiquitin-conjugating enzyme UbcH5b]-L-cysteine + [Ring finger domain of MEX3C]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[E2 ubiquitin-conjugating enzyme UBCh5b]-S-ubiquitinyl-L-cysteine + [Ring]-L-lysine
[E2 ubiquitin-conjugating enzyme UBCh5b]-L-cysteine + [Ring]-N6-ubiquitinyl-L-lysine
show the reaction diagram
[E2 ubiquitin-conjugating enzyme Ube2B]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine
[E2 ubiquitin-conjugating enzyme Ube2B]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[E2 ubiquitin-conjugating enzyme Ube2D]-S-ubiquitinyl-L-cysteine + [beta-catenin phosphoprotein]-L-lysine
[E2 ubiquitin-conjugating enzyme Ube2D]-L-cysteine + [beta-catenin phosphoprotein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[E2 ubiquitin-conjugating enzyme Ube2D]-S-ubiquitinyl-L-cysteine + [protein IkappaBalpha]-L-lysine
[E2 ubiquitin-conjugating enzyme Ube2D]-L-cysteine + [protein IkappaBalpha]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
-
?
[E2 ubiquitin-conjugating enzyme Ube2e1]-S-ubiquitinyl-L-cysteine + [BRCA1]-L-lysine
[E2 ubiquitin-conjugating enzyme Ube2e1]-L-cysteine + [BRCA1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
-
?
[E2 ubiquitin-conjugating enzyme Ube2w]-S-ubiquitinyl-L-cysteine + [BRCA1]-L-lysine
[E2 ubiquitin-conjugating enzyme Ube2w]-L-cysteine + [BRCA1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
-
-
-
?
[E2 ubiquitin-conjugating enzyme UbeD2]-S-ubiquitinyl-L-cysteine + [p53 transactivation domain]-L-lysine
[E2 ubiquitin-conjugating enzyme UbeD2]-L-cysteine + [p53 transactivation domain]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
-
?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [betaC1 protein]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + [betaC1 protein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
-
?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [FANCD2]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + [FANCD2]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
-
?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [KAT1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + [KAT1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
-
-
?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [MLO12-Myc]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor MLO12-Myc]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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?
[E2 ubiquitin-conjugating enzyme]-S-ubiquitinyl-L-cysteine + [MYB1]-L-lysine
[E2 ubiquitin-conjugating enzyme]-L-cysteine + [MYB1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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-
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?
[gp78-ubiquitin-carrier protein Ub2g2]-S-(ubiquitin)x-L-cysteine + HERP-L-lysine
[gp78-ubiquitin-carrier protein Ub2g2]-L-cysteine + HERP-N6-(ubiquitin)x-L-lysine
show the reaction diagram
HERP, ER-associated, short-lived protein that interacts with several E3 enzymes. Use of mouse Ube2g2 ubiquitin conjugating enzyme. Ube2g2/gp78-mediated polyubiquitination involves preassembly of Lys 48-linked ubiquitin chains at the catalytic cysteine of Ube2g2. The growth of Ube2g2-anchored ubiquitin chains seems to be mediated by an aminolysis-based transfer reaction between two Ube2g2 molecules that each carries a ubiquitin moiety in its active site. Polyubiquitination of a substrate can be achieved by transferring preassembled ubiquitin chains from Ube2g2 to a lysine residue in a substrate
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-
?
[gp78-ubiquitin-carrier protein Ub2g2]-S-[ubiquitinyl-N6-ubiquitinyl-L-lysine46]-L-cysteine + x-2 [gp78-ubiquitin-carrier protein Ub2g2]-S-ubiquitinyl-L-cysteine
[gp78-ubiquitin-carrier protein Ub2g2]-S-(ubiquitin)x-L-cysteine
show the reaction diagram
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-
-
?
[PirH2-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [PolH]-L-lysine
[PirH2-ubiquitin-carrier protein]-L-cysteine + [PolH]-N6-ubiquitinyl-L-lysine
show the reaction diagram
PolH, DNA polymerase eta, a Y family translesion polymerase and a target of the p53 tumor suppressor. PolH interacts with Pirh2 E3 ligase, via the polymerase-associated domain in PolH and the RING finger domain in Pirh2. PolH is recruited by Pirh2 and degraded by 20S proteasome in a ubiquitin-independent manner
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?
[PUB54-RING domain-ubiquitin-carrier protein Ubc13]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine
[PUB54-RING domain-ubiquitin-carrier protein Ubc13]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
ubiquitination activity is seen with both family-4/5 UBC enzymes, encoded by At2g16740 and At5g53300 and both family-13 UBC enzymes, encoded by At1g78870 and At16890
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?
[PUB54-RING domain-ubiquitin-carrier protein Ubc4/5]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine
[PUB54-RING domain-ubiquitin-carrier protein Ubc4/5]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
ubiquitination activity is seen with both family-4/5 UBC enzymes and both family-13 UBC enzymes
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[RFP1-ubiquitin-carrier protein E2]-S-ubiquitinyl-L-cysteine + [RFP1]-L-lysine
[RFP1-ubiquitin-carrier protein E2]-L-cysteine + [RFP1]-N6-ubiquitinyl-L-lysine
show the reaction diagram
in the presence of ubiquitin, ATP, rabbit E1, human E2 and recombinant isoform RFP1, accumulation of high molecular weight ubiquitinated RFP1 is detected, indicating its ability to autoubiquitinate
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[RING-E3-ubiquitin-carrier protein TRIM25]-S-ubiquitinyl-L-cysteine + [RIG-I]-L-lysine
[RING-E3-ubiquitin-carrier protein TRIM25]-L-cysteine + [RIG-I]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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the amino-terminal caspase recruitment domains CARDs of retinoic-acid-inducible gene RIG-I undergo robust ubiquitination induced by TRIM25 in mammalian cells. The carboxy-terminal SPRY domain of TRIM25 interacts with the N-terminal CARDs of RIG-I, this interaction effectively delivers the Lys 63-linked ubiquitin moiety to the N-terminal CARDs of RIG-I, resulting in a marked increase in RIG-I downstream signalling activity. The Lys 172 residue of RIG-I is critical for efficient TRIM25-mediated ubiquitination and for mitochondrial signaling protein MAVS binding, as well as the ability of RIG-I to induce antiviral signal transduction, the amino-terminal caspase recruitment domains CARDs of retinoic-acid-inducible protein RIG-I undergo robust ubiquitination induced by TRIM25 in mammalian cells. The carboxy-terminal SPRY domain of TRIM25 interacts with the N-terminal CARDs of RIG-I, this interaction effectively delivers the Lys 63-linked ubiquitin moiety to the N-terminal CARDs of RIG-I, resulting in a marked increase in RIG-I downstream signalling activity. The Lys 172 residue of RIG-I is critical for efficient TRIM25-mediated ubiquitination and for mitochondrial signaling protein MAVS binding, as well as the ability of RIG-I to induce antiviral signal transduction
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[RN181-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [RN181]-L-lysine
[RN181-ubiquitin-carrier protein]-L-cysteine + [RN181]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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in the presence of a ubiquitin-activating E1 enzyme, a ubiquitin-conjugating E2 enzyme, ubiquitin monomers and ATP, RN181 is self-ubiquitinated
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[RNF180-ubiquitin-carrier protein UbcH6]-S-ubiquitinyl-L-cysteine + [Zic2]-L-lysine
[RNF180-ubiquitin-carrier protein UbcH6]-L-cysteine + [Zic2]-N6-ubiquitinyl-L-lysine
show the reaction diagram
Zic2, belongs to the Zic family nuclear zinc finger proteins
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[RNF220-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [RF220]-L-lysine
[RNF220-ubiquitin-carrier protein]-L-cysteine + [RNF220]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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isoform RNF220 can bind ubiquitin-conjugating enzyme and mediate auto-ubiquitination of itself
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[RNF220-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [Sin3B]-L-lysine
[RNF220-ubiquitin-carrier protein]-L-cysteine + [Sin3B]-N6-ubiquitinyl-L-lysine
show the reaction diagram
Sin3B, a global regulator of gene transcription, which serves as an essential scaffold protein of the Sin3/HDAC corepressor complex
isoform RNF220 specifically interacts with Sin3B both in vitro and in vivo. Sin3B can be regulated by the ubiquitin-proteasome system. Co-expression of RNF220 and Sin3B promotes the ubiquitination and proteasomal degradation of Sin3B
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[RNF43-ubiquitin-carrier protein]-S-ubiquitinyl-L-cysteine + [RNF43]-L-lysine
[RNF43-ubiquitin-carrier protein]-L-cysteine + [RNF43]-N6-ubiquitinyl-L-lysine
show the reaction diagram
isoform RNF43 has autoubiquitylation activity. RNF43 is a RING finger-dependent E3 ligase that is selective for E2 enzymes UbcH5b and UbcH5c
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[TEB4-UBC7]-S-ubiquitinyl-L-cysteine + ubiquitin-L-lysine48
[TEB4-UBC7]-L-cysteine + ubiquitin-N6-ubiquitinyl-L-lysine48
show the reaction diagram
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formation of a ubiquitin dimer, where residue lysine48 is linked to the C-terminal carboxyl of another ubiquitin. The UBC7-dependent ubiquitin–ubiquitin linkage reaction requires the presence of the ubiquitin-activating enzyme E1 and ATP, suggesting that the activity requires the intermediate formation of an UBC7-ubiquitin thioester
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[TEB4-UBC7]-S-ubiquitinyl-L-cysteine + [acceptor protein]-L-lysine
[TEB4-UBC7]-L-cysteine + [acceptor protein]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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the isolated TEB4 RING domain catalyses ubiquitin ligation in vitro in a reaction that is ubiquitin Lys48-specific and involves ubiquitin-conjugating enzyme UBC7
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[TRIM22-ubiquitin-carrier protein UbcH5B]-S-ubiquitinyl-L-cysteine + [TRIM22]-L-lysine
[TRIM22-ubiquitin-carrier protein UbcH5B]-L-cysteine + [TRIM22]-N6-ubiquitinyl-L-lysine
show the reaction diagram
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isoform undergoes self-ubiq­uitylation in vitro in combination with the E2 enzyme UbcH5B, the ubiq­uitylation is dependent on its RING finger domain. TRIM22 is conjugated with poly-ubiq­uitin chains and stabilized by proteasome inhibitor in 293T cells
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[TRIM5-ubiquitin-carrier protein UbcH5B]-S-ubiquitinyl-L-cysteine + [TRIM5]-L-lysine
[TRIM5-ubiquitin-carrier protein UbcH5B]-L-cysteine + [TRIM5]-N6-ubiquitinyl-L-lysine
show the reaction diagram
TRIM5 functions as a RING-finger-type E3 ubiquitin ligase both in vitro and in vivo and ubiquitinates itself in cooperation with the E2 ubiquitin-conjugating enzyme UbcH5B. TRIM5 is monoubiquitinated, and ubiquitination does not lead to proteasomal degradation. Monoubiquitination may be a signal for TRIM5 to translocate from cytoplasmic bodies to the cytoplasm
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additional information
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