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2.3.2.24: (E3-independent) E2 ubiquitin-conjugating enzyme

This is an abbreviated version!
For detailed information about (E3-independent) E2 ubiquitin-conjugating enzyme, go to the full flat file.

Word Map on EC 2.3.2.24

Reaction

[(E3-independent) E2 ubiquitin-conjugating E2 enzyme]-S-monoubiquitinyl-L-cysteine
+
[acceptor protein]-L-lysine
=
[(E3-independent) E2 ubiquitin-conjugating enzyme]-L-cysteine
 +
[acceptor protein]-N6-monoubiquitinyl-L-lysine

Synonyms

arsenite-sensitive E2, biquitin carrier protein D4, biquitin-conjugating enzyme E2 T, CDC34, CLF_100482, E2 ubiquitin-conjugating enzyme, E2-230K, E2-EPF ubiquitin carrier protein, E2-EPF UCP, E2/E3 hybrid enzyme, E2230K, HIP2, N-terminus-conjugating E2, UB carrier protein (E2), Ubc1, Ubc3, UbcH10, UBE2A, UBE2B, UBE2C, UBE2D1, UBE2D2, UBE2D3, UBE2D4, UBE2E1, UBE2E2, UBE2E3, UBE2G1, UBE2G2, UBE2H, UBE2K, UBE2L3, UBE2N, UBE2O, UBE2Q2, UBE2R1, UBE2R2, UBE2S, UBE2T, UBE2V1, UBE2V2, UBE2W, ubiquitin carrier protein A, ubiquitin carrier protein B, ubiquitin carrier protein C, ubiquitin carrier protein D1, ubiquitin carrier protein D2, ubiquitin carrier protein D3, Ubiquitin carrier protein E1, ubiquitin carrier protein E2, ubiquitin carrier protein E3, Ubiquitin carrier protein G1, Ubiquitin carrier protein G2, ubiquitin carrier protein H, Ubiquitin carrier protein L3, ubiquitin carrier protein N, Ubiquitin carrier protein Q2, ubiquitin carrier protein R2, ubiquitin carrier protein S, ubiquitin carrier protein T, ubiquitin carrier protein W, ubiquitin-conjugating enzyme E2, ubiquitin-conjugating enzyme E2 A, ubiquitin-conjugating enzyme E2 B, ubiquitin-conjugating enzyme E2 C, ubiquitin-conjugating enzyme E2 D1, ubiquitin-conjugating enzyme E2 D2, ubiquitin-conjugating enzyme E2 D3, ubiquitin-conjugating enzyme E2 D4, ubiquitin-conjugating enzyme E2 E1, ubiquitin-conjugating enzyme E2 E2, ubiquitin-conjugating enzyme E2 E3, ubiquitin-conjugating enzyme E2 G1, ubiquitin-conjugating enzyme E2 G2, ubiquitin-conjugating enzyme E2 H, ubiquitin-conjugating enzyme E2 K, ubiquitin-conjugating enzyme E2 L3, ubiquitin-conjugating enzyme E2 N, ubiquitin-conjugating enzyme E2 O, Ubiquitin-conjugating enzyme E2 Q2, ubiquitin-conjugating enzyme E2 R1, ubiquitin-conjugating enzyme E2 R2, ubiquitin-conjugating enzyme E2 S, ubiquitin-conjugating enzyme E2 variant 1, ubiquitin-conjugating enzyme E2 variant 2, ubiquitin-conjugating enzyme E2 W, ubiquitin-conjugating enzyme E2-25 kDa, ubiquitin-conjugating enzyme E2-CDC34

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.24 (E3-independent) E2 ubiquitin-conjugating enzyme

Cloned

Cloned on EC 2.3.2.24 - (E3-independent) E2 ubiquitin-conjugating enzyme

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed and transformed into Escherichia coli (BL-21 DE3) using pET22 vector in frame to a C-terminal His6 tag
expression in Escherichia coli
gene UBE2O, cloned from liver, DNA and amino acid sequence determination and comparisons
gene UBE2O, recombinant expression of Strep-tagged UBE2O in HEK-293 cells, coexpression with FLAG-tagged BMAL1 transcription factor
recombinant expression of diversely tagged wild-type and mutant UCP enzymes in HEK-293T cells, the activity of the recombinant mutant enzymes varies dependent on the fusion tag, overview