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2.3.2.13: protein-glutamine gamma-glutamyltransferase

This is an abbreviated version!
For detailed information about protein-glutamine gamma-glutamyltransferase, go to the full flat file.

Word Map on EC 2.3.2.13

Reaction

protein glutamine
+
alkylamine
=
protein N5-alkylglutamine
 +
NH3

Synonyms

AcTG-1, BmTGA, chloroplast transglutaminase, chlTGase, cold active transglutaminase, cold-active transglutaminase, EPB42, factor XIII, factor XIIIa, fibrin stabilizing factor, fibrinoligase, Galphah, glutamine:amine gamma-glutamyl-transferase, glutaminylpeptide gamma-glutamyltransferase, glutamyltransferase, glutaminylpeptide gamma-, gpTGase 2, gTG2, hfXIIIa, hTG2, hTGase 1, hTGase 2, hTGase 3, hTGase 6, KALB, KalbTG, KALB_7456, Laki-Lorand factor, mammalian transglutaminase, microbial transglutaminase, microbial transglutaminases, MsTGase, MTG, MTG-TX, MTGase, mTGase 2, OlTGT, plastidial transglutaminase, polyamine transglutaminase, protein 4.2, protein-glutamine gamma-glutamyltransferase, R-glutaminyl-peptide:amine gamma-glutamyl transferase, R-glutaminylpeptide-amine gamma-glutamyltransferase, SCTG, SMTG, STG I, t-TG, TG-2, TG1, TG2, TG3, TG4, TG5, TG6, TG7, tGA, TGase, TGase 1, TGase 2, Tgase 3, TGase 6, Tgase II, TGase-2, TGase2, TGB, TGK1, TGK2, TGL, TGM1, TGM2, TGM3, TGM4, TGM5, TGM6, TGM7, TGZ, tgz15, TGZo, tissue transglutaminase, tissue transglutaminase 2, tissue-TG, transglutaminase, transglutaminase 1, transglutaminase 2, transglutaminase 3, transglutaminase 6, transglutaminase C, transglutaminase factor XIII, transglutaminase type II, transglutaminase-2, transglutaminase-like protein, transglutaminase2, tTG, tTG-2, type 2 transglutaminase, type I transglutaminase, type-2 transglutaminase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.13 protein-glutamine gamma-glutamyltransferase

Purification

Purification on EC 2.3.2.13 - protein-glutamine gamma-glutamyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
15% polyethylene glycol, DEAE-cellulos, isoelectric focusing
-
2 forms of epidermal enzyme
-
affinity chromatography on GTP-agarose
-
affinity chromatography on phenylalanine-Sepharose
-
amberlite CG-50, Blue Sepharose
-
ammonium sulfate, DEAE-Sepharose, Blue-Sepharose CL-6B, omega-aminohexyl agarose, alpha-casein agarose
-
brain transglutaminases NI and NII, DEAE-Sephacel, CM-cellulose, Q-Sepharose, FPLC gel filtration
-
CM-cellulose, Sephadex G-150
-
CM-Sepharose CL-6B, ammonium sulfate, DEAE-cellulose, Sephacryl S-300, DEAE-cosmogel, Zn-chelating Sepharose
-
co-purified with alpha-actinin
-
cytosolic tissue transglutaminase, DE52, heparin-agarose, casein-agarose
-
DEAE-cellulose, CM-cellulose, PO4-cellulose, Sephacryl S-200, Mono-S, cationic dermal isozymes A and B
-
DEAE-Sephacel, Mono-Q, Ultrogel AcA34
-
DEAE-unabsorbed transglutaminase, CM-Sepharose, heparin-Sepharose, Superdex 200, DEAE-absorbed transglutaminase, DEAE-Sephacel, heparin-Sepharose, hydroxyapatite, Superdex 200
-
epidermal transglutaminase, DEAE-cellulose, Sephadex G-75, CM-cellulose, gel filtration
-
erythrocyte transglutaminase
-
erythrocyte transglutaminase, DE-52 gel, Sephacryl S-300, Blue Sepharose, DEAE bio-gel
-
erythrocyte transglutaminase, DEAE cellulose, AcA 44, heparin-Sepharose, gel filtration
-
follicle transglutaminase, 6% agarose, DEAE-cellulose, CM-cellulose, 10% agarose
-
Fractogel EMD SO3- column chromatography
glutathione affinity column chromatography
glutathione Sepharose 4B resin chromatography
glutathione Sepharose column chromatography
heparin Sepharose column chromatography
HisTrap column chromatography and Resource Q column chromatography
-
HisTrap column chromatography, and Superdex 200 gel filtration
-
lens transglutaminase, DE-52, affinity chromatography on 42000 Da thermolytic fragment of human plasma fibronectin
-
liver transglutaminase, DEAE-cellulose, protamine, gel filtration
-
lung, matrix-bound enzyme solubilized, 3 isoforms: A, B and C
-
native enzyme 48fold from larvae by gel filtration and anion exchange chromatography
-
native enzyme about 10.3fold from Antarctic krill by ammonium sulfate fractionation, dialysis, ultrafiltration, and cation exchange chromatography, followed by a second ultrafiltration, precipitation with 80-90% ethanol, and freeze drying
-
native enzyme from liver by gel filtration and ultrafiltration
-
Ni-NTA affinity resin column chromatography, Mono Q column chromatography, and Superdex 200 gel filtration
-
Ni-NTA column chromatography
Ni-NTA column chromatography and HiTrap Q column chromatography
-
Ni-NTA column chromatography and Superdex 200 gel filtration
-
one-step purification by monoclonal antibody immunoadsorbent
-
overexpression of enzyme in Escherichia coli and purification from inclusion bodies, refolding by rapid dilution in a Ca2+- and guanidine-containing buffer
-
partial
partial purification by phenyl-Sepharose 6 column chromatography
-
plasma factor XIII, BaCl2, glycine, heat treatment, gel filtration, platelet factor XIII, DEAE-cellulose, hydroxyapatite
-
purification of extracellular high-salt resistent microbial transglutaminase, isozyme MTG-TX, by ethanol precipitation, cation exchange chromatography, hydrophobic interaction chromatography, and gel filtration, method optimization, overview
recombinant C-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant enzyme
recombinant enzyme expressed in Streptomyces lividans, protocol is applicable to laboratory and pilot-plant fermantations and gives 90-95% purity and 61-77% yield
-
recombinant enzyme, immunoaffinity chromatography, Mono-Q
-
recombinant expression of wild-type and codon-optimized, engineered enzymes from Pichia pastoris strain GS115 by ultrafiltration, gel filtration, anion exchange chromatography, and a second step of gel filtration
-
recombinant factor XIII-tissue transglutaminase chimeras
-
recombinant GST-tagged full-length TG2 V224 and G224 variants from Escherichia coli strain Rosetta 2 (DE3) by glutathione affinity chromatography
recombinant His-tagged full-length wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gelfiltration, followed by anion exchange chromatography, a second step of gel filtration, and ultrafiltration
recombinant His-tagged isozyme TG2 from Escherichia coli by nickel affinity chromatography and ultrafiltration
recombinant His-tagged soluble enzyme from Escherichia coli by nickel affinity chromatography, the tag is cleaved off by TEV protease followed by ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21Gold (DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and truncated mutant enzymes from Escherichia coli by nickel affinity chromatography, activation of inactive pro-MTG (mutant MTG and wild-type MTG) to a constant activity level is performed by adding trypsin at 30°C
recombinant protein expressed as inclusion bodies in Escherichia coli, application of an on-column refolding procedure based on cation SP fast flow chromatography
recombinant soluble enzyme from cell-free supernatant of Streptomyces lividans strain 1326 by ammonium sulfate fractionation, dialysis, and ultrafiltration
recombinant transglutaminase 1, DEAE-Sephacel, heparin-Sepharose, gel filtration
-
Resource S column chromatography and Sephadex G25 gel filtration
-
selectively solubilized with glycerol
-
Sephacryl S-300, hydroxyapatite
-
streptomycin sulfate, DEAE-cellulose, phenyl-Sepharose
-
strong-acid ion-exchange column
-
Talon column chromatography and Superdex 200 gel filtration
TALON resin column chromatography and Superdex 200 gel filtration
tissue-type transglutaminase
-
tissue-type transglutaminase, DE-52 cellulose, Sephacryl S-300, Mono Q
-
transglutaminase C
-
Triton X-100, deoxycholate or n-octylglucoside only 20-30% effective
-
TSKgel SuperSW3000 gel filtration