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2.3.2.13: protein-glutamine gamma-glutamyltransferase

This is an abbreviated version!
For detailed information about protein-glutamine gamma-glutamyltransferase, go to the full flat file.

Word Map on EC 2.3.2.13

Reaction

protein glutamine
+
alkylamine
=
protein N5-alkylglutamine
 +
NH3

Synonyms

AcTG-1, BmTGA, chloroplast transglutaminase, chlTGase, cold active transglutaminase, cold-active transglutaminase, EPB42, factor XIII, factor XIIIa, fibrin stabilizing factor, fibrinoligase, Galphah, glutamine:amine gamma-glutamyl-transferase, glutaminylpeptide gamma-glutamyltransferase, glutamyltransferase, glutaminylpeptide gamma-, gpTGase 2, gTG2, hfXIIIa, hTG2, hTGase 1, hTGase 2, hTGase 3, hTGase 6, KALB, KalbTG, KALB_7456, Laki-Lorand factor, mammalian transglutaminase, microbial transglutaminase, microbial transglutaminases, MsTGase, MTG, MTG-TX, MTGase, mTGase 2, OlTGT, plastidial transglutaminase, polyamine transglutaminase, protein 4.2, protein-glutamine gamma-glutamyltransferase, R-glutaminyl-peptide:amine gamma-glutamyl transferase, R-glutaminylpeptide-amine gamma-glutamyltransferase, SCTG, SMTG, STG I, t-TG, TG-2, TG1, TG2, TG3, TG4, TG5, TG6, TG7, tGA, TGase, TGase 1, TGase 2, Tgase 3, TGase 6, Tgase II, TGase-2, TGase2, TGB, TGK1, TGK2, TGL, TGM1, TGM2, TGM3, TGM4, TGM5, TGM6, TGM7, TGZ, tgz15, TGZo, tissue transglutaminase, tissue transglutaminase 2, tissue-TG, transglutaminase, transglutaminase 1, transglutaminase 2, transglutaminase 3, transglutaminase 6, transglutaminase C, transglutaminase factor XIII, transglutaminase type II, transglutaminase-2, transglutaminase-like protein, transglutaminase2, tTG, tTG-2, type 2 transglutaminase, type I transglutaminase, type-2 transglutaminase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.13 protein-glutamine gamma-glutamyltransferase

Cloned

Cloned on EC 2.3.2.13 - protein-glutamine gamma-glutamyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloned into pET21a and overexpressed in Escherichia coli BL21(DE3). The recombinant TGase is formed as inclusion bodies
cloning of factor XIII beta-subunit cDNA
-
cloning of factor XIII-tissue transglutaminase chimeras in Escherichia coli
-
cloning of placenta FXIIIa-gene cDNA
-
DNA and amino acid sequence determination and analysis, recombinant expression of wild-type and chimeric mutant enzymes in Streptomyces lividans strain 1326, Streptomyces TGase is secreted as a zymogen with an additional prosequence at the N-terminus
expressed in Corynebacterum glutamicum strain ATTC13869
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3)LysS cells
expressed in Escherichia coli K-12 cells
expressed in Escherichia coli Rosetta 2 (DE3) cells
expressed in Escherichia coli Rosetta 2 cells
expressed in Escherichia coli Rosetta 2 pLysS cells
-
expressed in Escherichia coli Rosetta cells
-
expressed in HEK-293T cells
-
expressed in HEK-293T/17, NIH-3T3, CHO-K1, and opossum kidney cells
expressed in inclusion bodies of Escherichia coli BL21(DE3) cells
-
expressed in INS-1E cells
-
expressed in Pichia pastoris
-
expression in Escherichia coli
expression in Escherichia coli as fusion protein with C-terminal His-tag
-
expression in Escherichia coli, with N-terminal His-tag
expression in SH-SY5Y, SK-N-SH, A172, U373MG, HeLa, MCF7 and HEK293 cells
-
expression in Streptomyces lividans 25-2
-
expression of transglutaminase 1 in Sf9 insect cells
-
expression of transglutaminase 3 in Sf9 cells
factor XIII, transglutaminase C and transglutaminase K
-
full-length gene is cloned from the head kidney by RT-PCR and rapid amplification of cDNA ends, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged soluble enzyme in Escherichia coli upon induction at low temperature. The recombinant AcTG-1 is not altered by proteolytic degradation
functional recombinant expression of N-terminally His-tagged soluble MTG in Escherichia coli strain Bl21 Tuner
functional recombinant expression of wild-type and engineered enzyme in Escherichia coli strain BL21(DE3), improvement of cloning and constitutive expression of soluble active mTG, overview. Usage of constitutive vector pET9a and a synthetic construct encoding the C-terminally His-tagged mTG thermostable variant S2P. Further increase in expression levels may be possible by coexpressing periplasmic secretory proteins, since secretion into the periplasm is frequently the limiting factor for production
gene mtg, cloning and recombinant expression of His-tagged wild-type and truncated mutant enzymes in pro-form and inactive zymogen in Escherichia coli strain Rosetta (DE3)
gene tg1, gene structure of tg1, the Streptomyces hygroscopicus TGase ORF is composed of a secretory signal peptide gene, a pro-peptide gene, and the mature TGase gene, the upstream and downstream sequences of the ORF contain a putative promoter and a putative terminator, respectively, recombinant enzyme expression from codon-optimized gene with engineered promoter in Streptomyces lividans strain TK24 yielding Streptomyces lividans TK24/pIJ86/tg1, the extracellular TGase is secreted. The complete promoter of TGase can be restricted to the sequence from -693 to -48 in tg1. Because the region from -198 to -148 negatively affects the expression, this region is deleted from the complete promoter (-693 to -48), which yields the TGase expression plasmid pTGO. Codon optimization of the TGase gene
gene Tgm1, quantitative RT-PCR expression analysis
gene tgz, recombinant expression of wild-type and codon-optimized enzyme in Pichia pastoris strain GS115 under control of two different promoter types, PFLD1 and PTEF1. Higher expression of TGZ is obtained under the induction of PFLD1
-
gene tgz15, recombinant overexpression in Arabidopsis thaliana Col-0 via transfection with Agrobacterium tumefaciens strain C58C1, enzyme activity fluorescence measurements in vivo, analysis of the ultrastructure of Arabidopsis plants, wild-type and transformed plants, phenotypes, overview
isoform PtTGB
-
isoform TGase 1
-
liver transglutaminase C
-
overexpression of liver transglutaminase in Escherichia coli, coexpression of Dank, DnaJ, GrpE or trigger factor increases solubility
-
overproduction of Bacillus subtilis Tgl-His6
-
recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinant expression of engineered mutant enzyme in Escherichia coli
recombinant expression of enzyme mutants in HeLa cells and in NIH 3T3 fibroblasts, nearly half of the cells die within 24 h. In contrast, introduction of wild-type tTG into those same cells does not induce cell death. Mutant R580L/C277A still induces cell death. Although ectopic expression of tTG mutant R580A in striatal cells is not sufficient to induce cell death, it does so in HeLa or NIH 3T3 cells
recombinant expression of GST-tagged full-length TG2 V224 and truncated G224 variants in Escherichia coli strain Rosetta 2 (DE3)
recombinant expression of His-tagged full-length wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of N-terminally His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21Gold (DE3)
recombinant expression of wild-type and chimeric mutant enzymes in Streptomyces lividans strain 1326, Streptomyces TGase is secreted as a zymogen with an additional prosequence at the N-terminus
recombinant expression of wild-type and mutant enzymes in Corynebacterium glutamicum strain ATCC 13032, subcloning in Escherichia coli strain DH5alpha
recombinant expression of wild-type and mutant enzymes in Escherichia coli
recombinant overexpression of His-tagged isozyme TG2 in Escherichia coli
recombinant overexpression of the enzyme in Pichia pastoris strain GS115
-
sub-cloned into a pET28 vector and overexpressed in Escherichia coli BL21. The recombinant proteins are present mainly as insoluble inclusion bodies. It can be effectively renatured in vitro and exhibits general TG enzyme characteristics
-
the pro-enzyme is expressed in Yarrowia lipolytica Po1g or Po1h strains
the recombinant enzyme protein is expressed in an active form in bacteria cultured at low temperature
transfection of HaCat cell and HEK-293 cell
-
transglutaminase C from macrophage
-