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2.3.2.10: UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase

This is an abbreviated version!
For detailed information about UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase, go to the full flat file.

Word Map on EC 2.3.2.10

Reaction

L-alanyl-tRNAAla
+
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
=
tRNAAla
 +
UDP-N-acetyl-alpha-D-muramoyl-L-alanyl-D-glutamyl-N6-(L-alanyl)-L-lysyl-D-alanyl-D-alanine

Synonyms

alanyl-transfer ribonucleate-uridine diphosphoacetylmuramoylpentapeptide transferase, alanyltransferase, alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-, FemX, FemXWv, FemXWv transferase, UDP-N-acetylmuramoylpentapeptide lysine N6-alanyltransferase, uridine diphosphoacetylmuramoylpentapeptide lysine N6-alanyltransferase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.2 Aminoacyltransferases
                2.3.2.10 UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase

Engineering

Engineering on EC 2.3.2.10 - UDP-N-acetylmuramoylpentapeptide-lysine N6-alanyltransferase

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
D109N
-
site-directed mutagenesis, loss of 99% activity compared to the wild-type
E205Q
-
site-directed mutagenesis, unaltered activity compared to the wild-type
E215Q
-
site-directed mutagenesis, loss of 15% activity compared to the wild-type
E316Q
-
site-directed mutagenesis, loss of 60% activity compared to the wild-type
E320Q
-
site-directed mutagenesis, loss of 96% activity compared to the wild-type
F70L
-
35% loss of activity
K36M
-
activity complete abolished
K36R
-
160-fold reduction of activity, 5.4-fold increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
R211K
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
Y103F
-
no effect on activity
Y215F
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
Y215L
-
insoluble protein
Y256F
-
2.6-fold reduction of activity
F70L
Weissella viridescens CIP 102810 / ATCC 12706
-
35% loss of activity
-
K36M
Weissella viridescens CIP 102810 / ATCC 12706
-
activity complete abolished
-
R211K
Weissella viridescens CIP 102810 / ATCC 12706
-
17000-fold reduction of activity, no effect on Km for Ala-tRNA, Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine higher than the highest sample concentration
-
Y103F
Weissella viridescens CIP 102810 / ATCC 12706
-
no effect on activity
-
Y215F
Weissella viridescens CIP 102810 / ATCC 12706
-
25-fold decrease of activity, increased Km for UDP-N-acetylmuramoyl-L-alanyl-D-glutamyl-L-lysyl-D-alanyl-D-alanine
-