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2.3.1.95: trihydroxystilbene synthase

This is an abbreviated version!
For detailed information about trihydroxystilbene synthase, go to the full flat file.

Word Map on EC 2.3.1.95

Reaction

3 malonyl-CoA +

4-coumaroyl-CoA
= 4 CoA +
trans-resveratrol
+ 4 CO2

Synonyms

AhSTS3, malonyl-CoA:4-coumaroyl-CoA malonyltransferase, resveratrol synthase, SbCHS8, SbSTS1, stilbene synthase, stilbene synthase 1, stilbene synthase 3, stilbene synthase-type polyketide synthase enzyme, STS, STS-type PKS enzyme, STS1, STS29, STS6, STS7, synthase, resveratrol, VIT_16s0100g01030, VIT_16s0100g01040, vst1

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.95 trihydroxystilbene synthase

Cloned

Cloned on EC 2.3.1.95 - trihydroxystilbene synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
co-expression of AhSTS3 with Sorghum bicolor O-methyltransferase OMT3, EC 2.1.1.240, in Arabidopsis thaliana and Nicotiana tabacum using the CaMV 35S promoter and the Agrobacterium tumefaciens transfection method, quantitative realtime RT-PCR expression analysis in transgenic leaf samples, phenotypic analysis of pCRO1 transformants, overview. Subcloning in Escherichia coli. Binary vector construction
expression in Arabidopsis thaliana
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expression in Escherichia coli
expression in Escherichia coli and Oryza sativa
expression of fusion enzymes in Escherichia coli
expression of wild-type, P375G and G374L mutant enzyme in Escherichia coli
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gene sts, phylogenetic analysis, sequence comparisons and homology modeling, overview. Expression of His6-tagged STS in Escherichia coli strain BL21 Star, functional co-expression with 4-coumaroyl:CoA ligase, 4CL, from Petroselinum crispum or Arabidopsis thaliana in Escherichia coli strain BW27784 leading to resveratrol biosynthesis
Humulus lupulus plants of the Tettnang variety are transformed with a gene encoding for STS from grapevine. Under the control of the constitutive 35S cauliflower mosaic virus promoter, expression of the transgene results in accumulation of resveratrol and high levels of its glycosylated derivatives in leaves and inflorescences. Piceid, the predominant derivative, reaches a concentration of up to 560 microg/g of fresh weight in hop cones, whereas no stilbenes are detected in nontransformed controls (wild type). In Humulus lupulus constitutive expression of sts interferes neither with plant development nor with the biosynthesis of secondary metabolites relevant for the brewing industry. Since resveratrol is a well-known phytoalexin and antioxidant, transgenic Humulus lupulus plants could display enhanced pathogen resistance against microbial pathogens, exhibit new beneficial properties for health, and open new venues for metabolic engineering
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PcRS gene , recombinant expression in Arabidopsis thaliana under the control of CaMV 35S promoter using the Agrobacterium tumefaciens LBA 4404 transfection method
Pisum sativum is transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the stilbene synthase (Vst1) from Vitis vinifera L. driven by its own elicitor-inducible promoter
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Populus alba is transformed with a construct containing cDNA insert encoding stilbene synthase under the control of the cauliflower mosaic virus 35S promoter and a kanamycin resistance gene
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production of the resveratrol beta-glucoside piceid by Saccharomyces cerevisiae by introduction of two key enzymes that are not present in Saccharomyces cerevisiae, coenzyme-A ligase and resveratrol synthase
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resveratrol synthase genes vst1 and vst2 from Vitis vinifera stably expressed in Triticum aestivum. Heterologous vst1 and vst2 genes retain their inducibility in wheat
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RS gene DNA and amino acid sequence determination and analysis, phylogenetic analysis, subcloning of the RS gene from peanut roots in Escherichia coli strain DH5alpha, expression in Escherichia coli strain BL21 (DE3). Transgenic expression of resveratrol synthase from Arachis hypogaea in Ipomoea batatas using Agrobacterium tumefaciens strain LBA4404 transfection method, transfection of leaves and stem sections, method, overview
SbCHS8 is overexpressed in transgenic Arabidopsis thaliana tt4 (transparent testa) mutants defective in chalcone synthase activities. SbCHS8 fails to complement the mutation
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sequence alignment with naringenin-chalcone synthase, EC 2.3.1.74
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the introduction of the stilbene synthase gene enhances the natural antiradical activity of Lycopersicon esculentum mill
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the stilbene synthase gene (vst1) from Vitis vinifera L. is cloned into pCLKSCLA25. The expression of vst1 gene contributes to the accumulation of trans-reveratrol from 3.4 to 8.7 microg/g fresh weight in different marker-free transgenic tomato lines
the stilbene synthase gene is isolated from Vitis vinifera L. is cloned under control of the seed-specific napin promotor and introduced into Brassica napus by Agrobacterium-mediated co-transformation together with a ds-RNA-interference construct deduced from the sequence of the key enzyme for sinapate ester biosynthesis biosynthesis, UDP-glucose:sinapate glucosyltransferase
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to construct a vector for STS expression in lettuce plant, a cDNA-encoding STS of Parthenocissus henryana is fused to the Cauliflower mosaic virus (CaMV) 35S promoter, and the bar gene is used as a selective marker gene. To increase the expression of STS, the expression cassette is flanked by matrix attachment regions. A high level of free trans-resveratrol is obtained in transgenic lettuce
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transformation of Carica papaya with Vitis vinifera stilbene synthase construct pVst1, containing the Vst1 gene and its pathogen-inducible promoter. RNA transcripts of stilbene synthase and resveratrol glycoside are induced in plant lines transformed with the grapevine pVst1 construct shortly after pathogen inoculation, and the transformed papaya lines exhibit increased resistance to Phytophthora palmivora
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