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2.3.1.91: sinapoylglucose-choline O-sinapoyltransferase

This is an abbreviated version!
For detailed information about sinapoylglucose-choline O-sinapoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.91

Reaction

1-O-sinapoyl-beta-D-glucose
+
choline
=
D-glucose
 +
Sinapoylcholine

Synonyms

1-O-sinapoyl-beta-glucose:choline sinapoyltransferase, AtSCT, BnSCT, SCT, ScT1, ScT2, sinapine synthase, sinapoylglucose-choline sinapoyltransferase, sinapoylglucose: choline sinapoyltransferase, sinapoylglucose:B. napus choline sinapoyltransferase, sinapoylglucose:choline sinapoyltransferase, sinapoylglucose:choline sinapoyltransferase., sinapoyltransferase, sinapoylglucose-choline

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.91 sinapoylglucose-choline O-sinapoyltransferase

Cloned

Cloned on EC 2.3.1.91 - sinapoylglucose-choline O-sinapoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, phylogenetic analysis
expression in a Saccharomyces cerevisiae expression system that lacks the carboxypeptodase Y gene and is defective in vacuolar targeting, resulting in secretion of heterologously expressed SCT
-
expression in Escherichia coli, the protein is exclusively present in inclusion bodies
-
expression in Saccharomyces cerevisiae
homology-based cloning strategy, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
structure model of 1-O-sinapoyl-beta-glucose:choline sinapoyltransferase based on homology with serine carboxypeptidases. The model confirms the main functional elements conserved within the SCPL protein family, i.e. hydrolase fold, catalytic triad, oxyanion hole and substrate recognition site