Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.9: acetyl-CoA C-acetyltransferase

This is an abbreviated version!
For detailed information about acetyl-CoA C-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.9

Reaction

2 acetyl-CoA =

CoA
+
acetoacetyl-CoA

Synonyms

2-methylacetoacetyl-CoA thiolase, 3-ketoacyl-CoA (T1)-like thiolase, 3-ketoacyl-CoA thiolase, 3-ketoacyl-coenzyme A thiolase, 3-oxothiolase, A1887, AACT, AACT1, AACT2, ACAT, ACAT1, ACAT2, ACCT, acetoacetyl CoA thiolase, acetoacetyl-CoA acetyltransferase, acetoacetyl-CoA C-acetyltransferase, acetoacetyl-CoA thiolase, acetoacetyl-CoA thiolase T2, acetyl coenzyme A thiolase, acetyl-CoA acetyltransferase, acetyl-CoA C-acetyltransferase, acetyl-CoA:N-acetyltransferase, acetyltransferase, acetyl coenzyme A, ACOAT, ACTRANS, AFUB_000550, AtoB, beta-acetoacetyl coenzyme A thiolase, beta-ketoacyl-CoA thiolase, beta-ketothiolase, CT, cytosolic acetoacetyl-CoA thiolase, cytosolic acetoacetyl-CoA thiolase 1, cytosolic acetoacetyl-CoA thiolase 2, ERG10, Erg10A, HFX_1022, HFX_1023, HFX_6003, HFX_6004, KACT, MmgA, Msed_0656, MSM-13 thiolase, OsAT1, phaA, ReH16_B0759, thiolase II, ThL, Tneu_0249, type II thiolase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.9 acetyl-CoA C-acetyltransferase

Purification

Purification on EC 2.3.1.9 - acetyl-CoA C-acetyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms: A and B
-
ammonium sulfate, acetone, acid treatment, CM-Sephadex, Sepharose 6B
-
ammonium sulfate, calcium phosphate gel, ethanol, partial purification
-
ammonium sulfate, DEAE-Sephacel, Blue Sepharose, AX 300 column
-
ammonium sulfate, DEAE-Sepharose, hydroxyapatite, phosphocellulose, Blue Sepharose
-
ammonium sulfate, Econo-Q, Superose 6, Mono Q, HA-Ultrogel, Mono Q
-
ammonium sulfate, hydroxylapatite, phosphocellulose
-
ammonium sulfate, Sephacryl S-200, superose 6, Mono Q
-
ammonium sulfate, Sephadex G-150, DEAE-Sephadex, QAE-Sephadex
-
constitutive mutant, thiolase II, heat treatment, phosphocellulose
-
cytosolic enzyme
-
cytosolic thiolase I
-
DEAE-cellulose, ammonium sulfate, gel filtration, DEAE-cellulose, Matrex gel green A
-
DEAE-Sepharose, butyl-Toyopearl, Cellulofine
-
His-Bind purification kit
hydroxyapatite, Superdex, Fractogel
-
isoenzymes A and B, ammonium sulfate, cellulose phosphate column, crystallization
-
liver enzyme, ammonium sulfate, calcium phosphate gel, phosphocellulose column, DEAE-cellulose, hydroxylapatite, Sephadex G-200
-
Ni-NTA agarose column chromatography and HiTrap Q ion exchange column chromatography
Ni-NTA column chromatography and Superdex 75 gel filtration
Ni2+-NTA bead chromatography and Superose 6 gel filtration
-
peroxisomal thiolase, DEAE-cellulose, phosphocellulose column, Blue-Sepharose
-
pH 5.5, DEAE-cellulose, calcium phosphate column, celulose phosphate column
-
poly ethylene glycol 6000, DEAE-cellulose 52, benzyl-Sepharose, hydroxylapatite
-
protamine sulfate, DEAE-cellulose, phosphocellulose, Sephadex G-200
-
Q-Sepharose, ammonium sulfate, Sephacryl S-200, Mono Q, FPLC S-300
-
Q-Sepharose, S-Sepharose, Superose 6, partially purifed
-
recombinant enzyme
-
recombinant His-tagged AACT 88fold to homogeneity by nickel affinity chromatography from Escherichia coli TOP10 cells
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to about 95% purity by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) to homogeneity by nickel affinity chromatography and gel filtration
recombinant His-tagged thiolase, Ni-affinity column
-
recombinant protein
single-step purification
-