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2.3.1.9: acetyl-CoA C-acetyltransferase

This is an abbreviated version!
For detailed information about acetyl-CoA C-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.9

Reaction

2 acetyl-CoA =

CoA
+
acetoacetyl-CoA

Synonyms

2-methylacetoacetyl-CoA thiolase, 3-ketoacyl-CoA (T1)-like thiolase, 3-ketoacyl-CoA thiolase, 3-ketoacyl-coenzyme A thiolase, 3-oxothiolase, A1887, AACT, AACT1, AACT2, ACAT, ACAT1, ACAT2, ACCT, acetoacetyl CoA thiolase, acetoacetyl-CoA acetyltransferase, acetoacetyl-CoA C-acetyltransferase, acetoacetyl-CoA thiolase, acetoacetyl-CoA thiolase T2, acetyl coenzyme A thiolase, acetyl-CoA acetyltransferase, acetyl-CoA C-acetyltransferase, acetyl-CoA:N-acetyltransferase, acetyltransferase, acetyl coenzyme A, ACOAT, ACTRANS, AFUB_000550, AtoB, beta-acetoacetyl coenzyme A thiolase, beta-ketoacyl-CoA thiolase, beta-ketothiolase, CT, cytosolic acetoacetyl-CoA thiolase, cytosolic acetoacetyl-CoA thiolase 1, cytosolic acetoacetyl-CoA thiolase 2, ERG10, Erg10A, HFX_1022, HFX_1023, HFX_6003, HFX_6004, KACT, MmgA, Msed_0656, MSM-13 thiolase, OsAT1, phaA, ReH16_B0759, thiolase II, ThL, Tneu_0249, type II thiolase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.9 acetyl-CoA C-acetyltransferase

Cloned

Cloned on EC 2.3.1.9 - acetyl-CoA C-acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
AACT, DNA and amino acid sequence determination and analysis, sequence comparison with Arabidopsis thaliana, expression of the active His-tagged enzyme in Escherichia coli TOP10 cells
expressed in Escherichia coli B834 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Saccharomyces cerevisiae strain D452-2
-
expression in Escherichia coli
expression in Sf9 cell
expression of cytosolic and peroxisomal thiolase I in Saccharomyces cerevisiae
-
gene ACT1, DNA and amino acid sequence determination and analysis, expression, expression analysis, phylogenetic analysis, overexpression in transgenic Arabidopsis thaliana plants using transfection with Agrobacterium tumefaciens
gene ACT2, DNA and amino acid sequence determination and analysis, expression analysis, phylogenetic analysis, overexpression in transgenic Arabidopsis thaliana plants using transfection with Agrobacterium tumefaciens
gene mmgA, encoded in the mmg operon, DNA and amino acid sequence determination and analysis, overexpression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
gene OSAT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of the His-tagged enzyme in Spodoptera frugiperda Sf9 cells using a baculovirus expression system, expression in the cytosol, rather than nucleus and mitochondria
gene ReH16_B0759, DNA and amino acid sequence determination and analysis, recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
overexpressed as soluble N-terminal His10-tagged protein in Escherichia coli
recombinant overexpression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
recombinantly expressed in Escherichia coli