Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.86: fatty-acyl-CoA synthase system

This is an abbreviated version!
For detailed information about fatty-acyl-CoA synthase system, go to the full flat file.

Word Map on EC 2.3.1.86

Reaction

acetyl-CoA
+ 7 malonyl-CoA + 14 NADPH + 14 H+ =
hexadecanoyl-CoA
 + 7 CoA + 7 CO2 + 14 NADP+ + 7 H2O

Synonyms

CpFas2, FAS, FAS1, fas2, fatty acid synthase, fatty-acyl-CoA synthase, yeast fatty acid synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.86 fatty-acyl-CoA synthase system

Engineering

Engineering on EC 2.3.1.86 - fatty-acyl-CoA synthase system

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
C1305A
-
peripheral SH-group defective
I306A/G1250S
mutant displays best production of hexanoic acid, accounting for 90% of measured short-chain fatty acids, yield is 20 mg/l
I306A/G1250S/M1250W
mutant displays increased synthesis of short-chain fatty acids, yield is up to 66 mg/l
I306A/R1834K/F1279Y
mutant displays best production of octanoic acid, yield is 48 mg/l
I306A/R1834K/G1250S
mutant displays increased synthesis of short-chain fatty acids, yield is up to 118 mg/l
R1834K
S180G
-
central SH-group defective
S5421Q
-
malonyl/palmitoyl transferase defective
S819Q
-
acyltransferase defective
T181G
-
central SH-group defective
A45C
-
about 5% reduction in overall enzyme activity
A45G
-
about 15% reduction in overall enzyme activity
A45W
-
about 10% reduction in overall enzyme activity
E41D
-
about 10% reduction in overall enzyme activity
E41K
-
no residual activity
V43I
-
no reduction in overall enzyme activity
additional information
-
FAS2 KO cells, construction of Fas2 disruptants influences virulence, unability to form normal biofilms