2.3.1.81: aminoglycoside 3-N-acetyltransferase

This is an abbreviated version!
For detailed information about aminoglycoside 3-N-acetyltransferase, go to the full flat file.

Reaction

Synonyms

3'-aminoglycoside acetyltransferase, 3-N-acetyltransferase, 3-N-aminoglycoside acetyltransferase, AAC, AAC (3)-IIIb, AAC(3), AAC(3)-Id, AAC(3)-Ig acetyltransferase, Aac(3)-IIIb, AAC(3)-IV, AAC(3)-XI, AAC(6')-APH(2''), AAC-IIIb, AAC-VIa, aac3-Ia, AAC3-IV aminoglycoside acetyltransferase, AacC-A7 acetyltransferase, aminoglycoside 3-acetyltransferase, aminoglycoside 3-acetyltransferase-IIIb, aminoglycoside 3-N-acetyltransferase, aminoglycoside 3-N-acetyltransferase type XI, aminoglycoside acetyltransferase, aminoglycoside acetytransferase, aminoglycoside N(3)-acetyltransferase III, aminoglycoside N3-acetyltransferase, aminoglycoside N3-acetyltransferase-IIIb, aminoglycoside N3-acetyltransferase-VIa, Eis, Eis2, Enhanced in Intracellular Survival, More, RbmI

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.81 aminoglycoside 3-N-acetyltransferase

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Results	in table
6278	AA Sequence
5	Application
1	CAS Registry Number
16	Cloned(Commentary)
1	Cofactor
4	Crystallization (Commentary)
9	Disease/ Diagnostics
7	General Information
3	General Stability
2	IC50 Value
29	Inhibitors
27	kcat/KM [mM/s]
2	Ki Value [mM]
71	KM Value [mM]
7	Localization
3	Metals/Ions
8	Molecular Weight [Da]
8	Natural Substrates/ Products (Substrates)
45	Organism
77	PDB ID
4	pH Optimum
1	pI Value
1	Posttranslational Modification
2	Protein Variants
7	Purification (Commentary)
1	Reaction
1	Reaction Type
38	Reference
1	Source Tissue
3	Specific Activity [micromol/min/mg]
2	Storage Stability
171	Substrates and Products (Substrate)
3	Subunits
52	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
1	Temperature Stability [°C]
66	Turnover Number [1/s]

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TURNOVER NUMBER [1/s]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.24 - 39.4

acetyl-CoA

6 entries

0.124 - 0.5

amikacin

4 entries

61

apramycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

3.5

butirosin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.3

butyryl-CoA

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.17

dibekacin

2 entries

0.041 - 0.182

gentamicin

3 entries

9.7

gentamicin C1

Enterobacter cloacae

-

pH 7.6, 25°C

758305

18.4

gentamicin C2

Enterobacter cloacae

-

pH 7.6, 25°C

758305

40

gentamycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.012 - 80

kanamycin A

11 entries

0.23 - 55

kanamycin B

5 entries

33.8

lividomycin A

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

5.7 - 9

malonyl-CoA

2 entries

10.2

n-propionyl-CoA

Pseudomonas aeruginosa

-

cosubstrate kanamycin A, pH 7.6, 25°C

718871

10 - 60

neomycin

2 entries

0.076 - 9.5

neomycin B

3 entries

34

neomycin E

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

32

netilmicin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

15 - 33.6

paromomycin

2 entries

33

propionyl-CoA

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

23.5 - 146

ribostamycin

3 entries

0.121 - 24.5

sisomicin

3 entries

46 - 51

sisomycin

3 entries

0.089 - 162

tobramycin

6 entries

0.24

acetyl-CoA

Corynebacterium striatum

A0A0K2X0F2

pH 6.0, 37°C

735448

0.24

acetyl-CoA

Corynebacterium striatum

A0A0K2X0F2

recombinant His-tagged enzyme, pH 6.0, 37°C

735448

2.2

acetyl-CoA

Mycobacteroides abscessus

B1MKV6

25°C, pH not specified in the publication

756710

10.5

acetyl-CoA

Pseudomonas aeruginosa

-

cosubstrate neomycin B, pH 7.6, 25°C

718871

39

acetyl-CoA

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

39.4

acetyl-CoA

Pseudomonas aeruginosa

-

cosubstrate kanamycin A, pH 7.6, 25°C

718871

0.124

amikacin

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.126

amikacin

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.146

amikacin

Escherichia coli

-

pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.5

amikacin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.17

dibekacin

Corynebacterium striatum

A0A0K2X0F2

pH 6.0, 37°C

735448

0.17

dibekacin

Corynebacterium striatum

A0A0K2X0F2

recombinant His-tagged enzyme, pH 6.0, 37°C

735448

0.041

gentamicin

Escherichia coli

-

pH 6.6, with malonyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.123

gentamicin

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.182

gentamicin

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.012

kanamycin A

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET28a

703205

0.017

kanamycin A

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET28a

703205

0.044

kanamycin A

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps

703205

0.134

kanamycin A

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.144

kanamycin A

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps

703205

0.163

kanamycin A

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector Int-pET19b-pps

703205

0.3

kanamycin A

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.559

kanamycin A

Escherichia coli

-

pH 6.6, with acetyl-CoA, untagged enzyme expressed from vector Int-pET19b-pps

703205

51

kanamycin A

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

51.4

kanamycin A

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

80

kanamycin A

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.23

kanamycin B

Corynebacterium striatum

A0A0K2X0F2

pH 6.0, 37°C

735448

0.23

kanamycin B

Corynebacterium striatum

A0A0K2X0F2

recombinant His-tagged enzyme, pH 6.0, 37°C

735448

14.5

kanamycin B

Enterobacter cloacae

-

pH 7.6, 25°C

758305

54.5

kanamycin B

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

55

kanamycin B

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

5.7

malonyl-CoA

Pseudomonas aeruginosa

-

cosubstrate kanamycin A, pH 7.6, 25°C

718871

9

malonyl-CoA

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

10

neomycin

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

60

neomycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.076

neomycin B

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.188

neomycin B

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

9.5

neomycin B

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

15

paromomycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

33.6

paromomycin

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

23.5

ribostamycin

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

24

ribostamycin

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

146

ribostamycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.121

sisomicin

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.173

sisomicin

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

24.5

sisomicin

Enterobacter cloacae

-

pH 7.6, 25°C

758305

46

sisomycin

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

46.3

sisomycin

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

51

sisomycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007

0.089

tobramycin

Escherichia coli

-

pH 6.6, with n-propionyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

0.179

tobramycin

Escherichia coli

-

pH 6.6, with acetyl-CoA, His-tagged enzyme expressed from vector pET22b

703205

43.5

tobramycin

Enterobacter cloacae

-

pH 7.6, 25°C

758305

47.8

tobramycin

Pseudomonas aeruginosa

-

pH 7.6, 25°C

718870

48

tobramycin

Pseudomonas aeruginosa

Q51405

pH 7.6, 25°C

735678

162

tobramycin

Escherichia coli

Q306W4

in 50 mM HEPES, pH 7.5 and 0.1 mM 4,4'-dithiodipyridine, at pH 7.8 and 25°C

672007