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2.3.1.81: aminoglycoside 3-N-acetyltransferase

This is an abbreviated version!
For detailed information about aminoglycoside 3-N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.81

Reaction

acetyl-CoA
+
a 2-deoxystreptamine antibiotic
=
CoA
 +
N3-acetyl-2-deoxystreptamine antibiotic

Synonyms

3'-aminoglycoside acetyltransferase, 3-N-acetyltransferase, 3-N-aminoglycoside acetyltransferase, AAC, AAC (3)-IIIb, AAC(3), AAC(3)-Id, AAC(3)-Ig acetyltransferase, Aac(3)-IIIb, AAC(3)-IV, AAC(3)-XI, AAC(6')-APH(2''), AAC-IIIb, AAC-VIa, aac3-Ia, AAC3-IV aminoglycoside acetyltransferase, AacC-A7 acetyltransferase, aminoglycoside 3-acetyltransferase, aminoglycoside 3-acetyltransferase-IIIb, aminoglycoside 3-N-acetyltransferase, aminoglycoside 3-N-acetyltransferase type XI, aminoglycoside acetyltransferase, aminoglycoside acetytransferase, aminoglycoside N(3)-acetyltransferase III, aminoglycoside N3-acetyltransferase, aminoglycoside N3-acetyltransferase-IIIb, aminoglycoside N3-acetyltransferase-VIa, Eis, Eis2, Enhanced in Intracellular Survival, More, RbmI

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.81 aminoglycoside 3-N-acetyltransferase

Cloned

Cloned on EC 2.3.1.81 - aminoglycoside 3-N-acetyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in the pET-23a(+) expression vector
expression in Escherichia coli
expression in Escherichia coli DH5alpha
expression in Escherichia coli K802N by mating
expression of C- or N-terminally His-tagged enzyme from vectors pET22b, and pET28a or Int-pET19b-pps, respectively
-
gene aac(3)-Id, in a class 1 integron
-
gene aacC2, genotyping
gne aac(3)-XI, DNA and amino acid sequence determination and analysis, genomic organization, the aac(3)-XI gene is located downstream from a gene for a transposase (TnpCx) and upstream from that for a two-component system sensor kinase, the sequence upstream from aac(3)-XI does not reveal any putative promoter, recombinant expression of His-tagged enzyme in Escherichia coli strains TOP10 and BL21 Star(DE3)
plasmid transfer pIJ702 to Streptomyces lividans TK21
-
Pseudomonas aeruginosa plasmid pPK237 transferred to and expressed in Escherichia coli K12
-
wild-type and truncated enzymes expressed in Escherichia coli
-