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2.3.1.8: phosphate acetyltransferase

This is an abbreviated version!
For detailed information about phosphate acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.8

Reaction

acetyl-CoA
+
phosphate
=
CoA
+
acetyl phosphate

Synonyms

acetyl-S-CoA: orthophosphate acetyltransferase, acetyltransferase, phosphate, aster yellows witches'-broom phosphotransacetylase-like enzyme, AYWB-PduL, EutD, Moth_0864, Moth_1181, pduL1, pduL2, PGN_1179, phosphate acetyltransferase, phosphoacylase, phosphotransacetylase, phosphotransacetylase EutD, phosphotransacetylase Pta, PTA, Pta-1, Pta-2, PtaII1, PtaII2

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.8 phosphate acetyltransferase

Purification

Purification on EC 2.3.1.8 - phosphate acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography, 73% pure
-
apparent homogeneity
-
enzyme is associated with the pyruvate dehydrogenase complex
-
fusion protein with beta-galactosidase
-
HisTrap HP Ni2+ affinity column chromatography
-
homogeneity, biotinylated fusion protein
-
Niquel-NTA column chromatography
-
recombinant enzyme from Escherichia coli
-
recombinant protein using His-tag