Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.8: phosphate acetyltransferase

This is an abbreviated version!
For detailed information about phosphate acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.8

Reaction

acetyl-CoA
+
phosphate
=
CoA
+
acetyl phosphate

Synonyms

acetyl-S-CoA: orthophosphate acetyltransferase, acetyltransferase, phosphate, aster yellows witches'-broom phosphotransacetylase-like enzyme, AYWB-PduL, EutD, Moth_0864, Moth_1181, pduL1, pduL2, PGN_1179, phosphate acetyltransferase, phosphoacylase, phosphotransacetylase, phosphotransacetylase EutD, phosphotransacetylase Pta, PTA, Pta-1, Pta-2, PtaII1, PtaII2

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.8 phosphate acetyltransferase

General Stability

General Stability on EC 2.3.1.8 - phosphate acetyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
GENERAL STABILITY
ORGANISM
UNIPROT
LITERATURE
(NH4)2SO4, Na2SO4, NaCl or KCl stabilizes
-
50-60% loss of activity after dialysis for 8 h against 0.05 M Tris buffer, Fe2+ and dithiothreitol stabilize
-
activity is lost upon dialysis and cannot be restored by addition of known cofactors or crude boiled extracts
-
ammonium sulfate and potassium phosphate stabilize
-
dilute solutions are instable, in frozen state stable
-
divalent cations, e.g. FeSO4, Fe(NH4)2SO4, MgCl2, MnCl2, MgSO4 or ATP increase lability
-
ethylene glycol 20% v/v stabilizes
-
increased stability is obtained by adding a reducing agent and a component of the reaction
-
labile in dilute solutions or at elevated temperatures
-
lyophilization causes almost complete loss of enzyme activity
-
MgCl2 destabilizes
-
NH4Cl sligthly stabilizes
-
repeated freezing and thawing inactivates
-
stabilized by addition of 200 mM ammonium sulfate and 2-5 mM mercaptans to the extraction buffer
-
sulfate and phosphate partially protect against heat inactivation
-