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2.3.1.75: long-chain-alcohol O-fatty-acyltransferase

This is an abbreviated version!
For detailed information about long-chain-alcohol O-fatty-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.75

Reaction

acyl-CoA
+
a long-chain alcohol
=
CoA
 +
a long-chain ester

Synonyms

acyl CoA wax alcohol acyltransferase 1, acyl CoA wax alcohol acyltransferase 2, acyl CoA:long-chain fatty alcohol O-acyltransferase, acyl-CoA wax alcohol acyltransferase, acyl-CoA wax alcohol acyltransferase 2, acyl-CoA wax alcohol acyltransferases, acyl-CoA:alcohol transacylase, acyl-CoA:fatty acyl alcohol acyltransferase, acyl-CoA:retinol acyltransferase, acyltransferase, long-chain alcohol, ArAT, ATF, ATF1, AtfA, AtfA1, AtfG25, AWAT, AWAT 1, AWAT 2, AWAT1, AWAT2, bifunctional WS/DGAT, EgWSD2, EgWSD5, fatty acyl-coenzyme A:fatty alcohol acyltransferase, isoprenoid wax ester synthase, Maqu_0168, MFAT, More, multifunctional O-acyltransferase, Rha1, TrWSD4, TrWSD5, unspecific bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, wax ester synthase, wax ester synthase/acyl CoA:diacylglycerol acyltransferase, wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, wax ester synthase/acyl-CoA diacylglycerol acyltransferase, wax ester synthase/acyl-CoA:diacylglycerol acyltransferase, wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase, wax ester synthase/diacylglycerol acyltransferase, wax ester synthases/acyl-CoA:diacylglycerol acyltransferase, wax monoester synthase, wax synthase, wax-ester synthase, waxester/diacylglycerol acyltransferase, WE synthase, WE synthases/acyl-CoA:diacylglycerol acyltransferase, WS, WS/DGAT, WS2, WSD1, WSD2, WSD4, WSD5

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.75 long-chain-alcohol O-fatty-acyltransferase

Cloned

Cloned on EC 2.3.1.75 - long-chain-alcohol O-fatty-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells
cloning of cDNA, DNA sequence analysis, construction of plasmid to express the cDNA in transgenic Arabidopsis thaliana plants
delta integration in Saccharomyces cerevisiae, resulting in 1–6 copies of the integration construct per genome
Marinobacter nauticus
DNA and amino acid sequence determination and analysis of wax synthase analogous genes, expression patterns, overview
expressed in Arabidopsis thaliana fad2 fae1 double mutant
expressed in Escherichia coli C41(DE3) cells
-
expressed in Escherichia coli Rosetta (DE3) cells and Saccharomyces cerevisiae quadruple mutant H1246
expressed in Saccharomyces cerevisiae
expressed in Saccharomyces cerevisiae quadruple mutant strain H1246
expressed in Saccharomyces cerevisiae strain H1246
expressed in seeds of Arabidopsis thaliana and Camelina sativa
-
expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression in Escherichia coli
expression in Saccharomyces cerevisiae
expression in Saccharomyces cerevisiae and Escherichia coli
-
functional expression in Escherichia coli strain BL21(DE3)
-
gene atfA, DNA and amino acid sequence determination and analysis, WS/DGAT family sequence comparisons and phylogenetic relationships, overview, expression in Escherichia coli as untagged or C-terminally His6-tagged enzyme
-
gene atfA, expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3)
-
gene atfG25, DNA and amino acid sequence determination and analysis, genomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3)
-
gene AWAT1, i.e. DGA2, location on the X chromosome Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae
gene DC3, DNA and amino acid sequence determination and analysis, genetic organization and phylogenetics, expression in Saccharomyces cerevisiae
gene DC4, DNA and amino acid sequence determination and analysis, genetic organization and phylogenetics, expression in Saccharomyces cerevisiae
gene DC4, i.e. AWAT2, location on the X chromosome Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae
gene DGAT2, DNA and amino acid sequence determination and analysis, genetic organization and phylogenetics, expression in Saccharomyces cerevisiae
gene WSD2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells
gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast
gene WSD5, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells
gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast
genes atfA1 and atfA2, expression in Escherichia coli strain BL21(DE3)
-
genes with high homologies to the Acinetobacter sp. ADP1 atfA are identified in the genome databases of several Gram-negative strains, including marine bacteria like Hahella chejuensis, psychrophilic strains like Psychrobacter sp., Polaromonas sp. and Bradyrhizobium japonicum. Ten atfA homologous genes are identified in the genome database of Arabidopsis thaliana
isozyme AWAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
isozyme AWAT2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
isozymes AWAT1 and AWAT2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis
-
MFAT, DNA and amino acid sequence determination and analysis, localized on the X chromosome, expression of the N-terminally FLAG-tagged enzyme in Spodoptera frugierda Sf9 cells using the baculovirus transfection system, or in COS-7 cells
screening, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109
Marinobacter nauticus
-