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2.3.1.74: chalcone synthase

This is an abbreviated version!
For detailed information about chalcone synthase, go to the full flat file.

Word Map on EC 2.3.1.74

Reaction

3 malonyl-CoA +

4-coumaroyl-CoA
= 4 CoA +
naringenin chalcone
 + 3 CO2

Synonyms

6'-deoxychalcone synthase, anther-specific chalcone synthase-like enzyme, ASCL1, ASCL2, chalcone synthase, chalcone synthase 6, chalcone synthase 7, chalcone synthase C2-Idf-I, chalcone synthase C2-Idf-II, chalcone synthetase, CHS, CHS var 2, chs-1, CHS-7, CHS01, CHS02, CHS03, CHS04, CHS05, CHS06, CHS07, CHS08, CHS088, CHS09, Chs1, CHS10, CHS11, CHS12, CHS13, CHS14, Chs2, CHS2 chalcone synthase, CHS3, CHS4, CHS444, CHS5, CHS6, CHS7, CHS768, CHS_H1, Cs-COR126, DOCS, EC 2.3.1.120, flavanone synthase, flavanone synthetase, GmIRCHS, MdCHS2, More, naringenin-chalcone synthase, naringenin-chalcone synthase 6, PgCHS, PKS1, Q7Y1Y0, RinPKS1, RiPKS4, RiPKS5, synthase, flavanone, vsad1

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.74 chalcone synthase

Cloned

Cloned on EC 2.3.1.74 - chalcone synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
an octaploid (Fragaria x ananassa cv. Calypso) genotype of strawberry is transformed with an antisense chalcone synthase (CHS) gene construct using a ripening related CHS cDNA from Fragaria x ananassa cv. Elsanta under the control of the constitutive CaMV 35S promoter via Agrobacterium tumefaciens. Out of 25 transgenic lines, nine lines showed a reduction in CHS mRNA accumulation of more than 50% as compared to the untransformed cv. Calypso control
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expressed in Arabidopsis thaliana mesophyll protoplasts
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) pLysS cells
expressed in Escherichia coli BL21 cells
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21-Rosetta (DE3) cells
expressed in Escherichia coli DH5alpha cells
expressed in Escherichia coli Rosetta-gami (DE3) and BL21-Rosetta (DE3) cells
expressed in Nicotiana tabacum
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expressed in Nicotiana tabacum leaves
expression in Escherichia coi.
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expression in Escherichia coli
expression in Escherichia coli JM109
expression in Escherichia coli, site directed mutagenesis, sequence alignment with EC 2.3.1.95
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expression of the coding sequence of Pchs in Nicotiana tabacum can lead to darker flower limbs than seen in controls, and some transgenic plants exhibited abnormality in growth of pollen tubes
fusion protein with glutathione S-transferase
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GCHS2 and GCHS26 with different enzymatic and structural properties
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gene CHS and mutant gene CHS-wf, DNA and amino acid sequence determination and analysis, expression analysis by semi-quantitative RT-PCR
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gene CHS, DNA and amino acid sequence determination and analysis, expression analysis
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gene CHS, realtime PCR expression analysis
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gene CHS-A, DNA and amino acid sequence determination and analysis, molecular phylogram analysis of SbCHS family and CHS families from other plants, phylogenetic tree
gene CHS-B, DNA and amino acid sequence determination and analysis, molecular phylogram analysis of SbCHS family and CHS families from other plants, phylogenetic tree
gene CHS-C, DNA and amino acid sequence determination and analysis, molecular phylogram analysis of SbCHS family and CHS families from other plants, phylogenetic tree
gene CHSL1, DNA and amino acid sequence determination and analysis, pattern of expression, overview
gene CsCHS-bo, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenetic analysis, cloning and expression in Escherichia coli strain DH5alpha
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hairy roots, transformed with the soybean chalcone synthase (CHS6) or isoflavone synthase (IFS2) genes, with dramatically decreased capacity to synthesize isoflavones are produced to determine what effects these changes would have on susceptibility to a fungal pathogen
infiltration of Nicotiana benthamiana leaves with chs_H1 promoter/GUS chimeras leads to a 24.8-fold increase of the GUS activity when coinfiltrated with the pap1 gene. Coinfiltration of the native chs_H1 gene with pap1 leads to an increased accumulation of chs_H1 mRNA. Transgenic lines of Petunia hybrida expressing the pap1 gene showed unusual patterns of UV-A-inducible pigmentation and anthocyanin accumulation in parenchymatic and medulla cells. Infiltration of transgenic leaves of Petunia hybrida with chs_H1 and pap1 genes arranged as a tandem led to quick pigmentation within 12 h after UV-A irradiation
introduction of the phenylpropanoid pathway with the genes for phenylalanine ammonia lyase (PAL) from Rhodosporidium toruloides, 4-coumarate:coenzyme A (CoA) ligase (4CL) from Arabidopsis thaliana, and chalcone synthase (CHS) from Hypericum androsaemum into two Saccharomyces cerevisiae strains, namely, AH22 and a pad1 knockout mutant. Each gene is cloned and inserted into an expression vector under the control of a separate individual GAL10 promoter
isoforms
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isolation and characterization of cDNA sequences encoding yellow lupin chalcone synthase. Chalcone synthase is encoded by at least two genes. The two sequences may have evolved by gene duplication
overexpressed in Escherichia coli
overexpression in Escherichia coli as glutathione-S-transferase fusion protein, wild-type and mutant enzymes: L263M, F265Y, G256A, S338G, L263M/F265Y, G256A/S338G, L263M/S338G, F265Y/S338G, L263M/F265Y/S338G, G256A/L263M/F265Y, G256A/L263M/F265Y/S338G
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recombinant Escherichia coli cells containing four genes for a phenylalanine ammonia-lyase, cinnamate/coumarate:CoA ligase, chalcone synthase, and chalcone isomerase, in addition to the acetyl-CoA carboxylase, have been established for efficient production of (2S)-naringenin from tyrosine and (2S)-pinocembrin from phenylalanine. Further introduction of the flavone synthase I gene from Petroselinum crispum under the control of the T7 promoter and the synthetic ribosome-binding sequence in pACYCDuet-1 causes the Escherichia coli cells to produce flavones: apigenin (13 mg/l) from tyrosine and chrysin (9.4 mg/l) from phenylalanine
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wild type and mutants
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wild type enzyme and HvCHS2 with different substrate requirements
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