2.3.1.7: carnitine O-acetyltransferase

This is an abbreviated version!
For detailed information about carnitine O-acetyltransferase, go to the full flat file.

Reaction

Synonyms

acetyl-CoA-carnitine O-acetyltransferase, acetylcarnitine transferase, acuJ, CarAc, CARAT, carnitine acetyl coenzyme A transferase, carnitine acetyl transferase, carnitine acetylase, carnitine acetyltransferase, carnitine acetyltransferase CAT2, carnitine acetyltransferase Cat2p, carnitine acetyltransferase Yat1p, carnitine acetyltransferase Yat2p, carnitine-acetyl-CoA transferase, CAT, CAT2, CATC, CRAT, CT-CAT, CTN1, CTN2, CTN3, H-CAT, P-CAT, S-CAT1, S-CAT2, Yat1

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.7 carnitine O-acetyltransferase

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Results	in table
6	Activating Compound
126	AA Sequence
12	Application
1	CAS Registry Number
19	Cloned(Commentary)
11	Crystallization (Commentary)
173	Disease/ Diagnostics
3	Expression
10	General Information
6	General Stability
3	IC50 Value
116	Inhibitors
37	Ki Value [mM]
188	KM Value [mM]
183	Localization
73	Molecular Weight [Da]
480	Natural Substrates/ Products (Substrates)
563	Organism
11	PDB ID
16	pH Optimum
6	pH Range
2	pH Stability
1	Posttranslational Modification
23	Protein Variants
57	Purification (Commentary)
5	Reaction
1	Reaction Type
156	Reference
233	Source Tissue
30	Specific Activity [micromol/min/mg]
12	Storage Stability
952	Substrates and Products (Substrate)
46	Subunits
97	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
1	Temperature Range [°C]
3	Temperature Stability [°C]
24	Turnover Number [1/s]

KM Value

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KM Value on EC 2.3.1.7 - carnitine O-acetyltransferase

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KM VALUE [mM]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

1.6

(-)-norcarnitine

Sus scrofa

-

-

487398

0.31

(-)carnitine

Sus scrofa

-

-

487398

50

(trishydroxymethyl)glycine

Columba sp.

-

-

487401

0.012 - 0.498

acetyl-CoA

30 entries

0.307

acetyl-DL-carnitine

Camelus dromedarius

-

-

487422

0.019 - 0.639

acetylcarnitine

7 entries

0.015 - 0.499

butyryl-CoA

8 entries

0.48 - 0.66

butyrylcarnitine

2 entries

0.0035 - 300

carnitine

26 entries

1.1 - 86.4

choline

6 entries

0.0328 - 0.1134

CoA

4 entries

0.009 - 2

CoASH

25 entries

0.0287 - 0.064

decanoyl-CoA

2 entries

0.64 - 0.66

decanoylcarnitine

2 entries

40

DL-beta-hydroxy-gamma-aminobutyrate

Sus scrofa

-

-

487398

0.72 - 3.3

DL-carnitine

2 entries

3.2

DL-norcarnitine

Sus scrofa

-

-

487398

0.02 - 0.112

hexanoyl-CoA

8 entries

2.28 - 2.51

hexanoylcarnitine

2 entries

0.049

isobutyryl-CoA

Bos taurus

-

-

487407

0.039 - 0.244

L-(-)-carnitine

7 entries

0.1 - 35.9

L-carnitine

17 entries

0.041

n-butyryl-CoA

Bos taurus

-

-

487407

0.071

n-valeryl-CoA

Bos taurus

-

-

487407

0.3 - 0.36

O-acetyl-L-carnitine

2 entries

-

0.011 - 0.0503

octanoyl-CoA

4 entries

1.27 - 1.62

octanoylcarnitine

3 entries

0.0072

palmitoyl-CoA

Rattus norvegicus

-

mutant enzyme D356A/M564G

672172

0.014 - 0.185

propionyl-CoA

8 entries

0.65

propionylcarnitine

Homo sapiens

-

-

487416

additional information

additional information

Rattus norvegicus

-

Km acetylcarnitine 1.25 mg/ml

487432

-

0.012

acetyl-CoA

Bos taurus

-

sperm enzyme

487407

0.014

acetyl-CoA

Candida albicans

-

peroxisomal enzyme

487427

0.015

acetyl-CoA

Bos taurus

-

-

487407

0.0177

acetyl-CoA

Saccharomyces cerevisiae

-

-

487418

0.02

acetyl-CoA

Bos taurus

-

heart enzyme

487407

0.021

acetyl-CoA

Homo sapiens

-

-

487427

0.021

acetyl-CoA

Mus musculus

P47934

21°C, pH 7.8, wild-type enzyme

674651

0.0213

acetyl-CoA

Homo sapiens

-

-

487416, 487423

0.023

acetyl-CoA

Rattus norvegicus

Q704S8

recombinant enzyme from yeast, pH 7.8, 30°C

659372

0.023

acetyl-CoA

Rattus norvegicus

-

mutant enzyme D356A/M564G

672172

0.032

acetyl-CoA

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast, pH 7.8, 30°C

659372

0.0324

acetyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme H343E

674651

0.036

acetyl-CoA

Candida tropicalis

-

mitochondrial enzyme

487406, 487427

0.038

acetyl-CoA

Candida albicans

-

mitochondrial enzyme

487427

0.042

acetyl-CoA

Candida tropicalis

-

peroxisomal enzyme

487406, 487427

0.042

acetyl-CoA

Homo sapiens

P43155

wild type enzyme, pH 8.0

659815

0.044

acetyl-CoA

Camelus dromedarius

-

-

487422

0.0596

acetyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme S554A/M564G

674651

0.064

acetyl-CoA

Homo sapiens

P43155

F545Y mutant enzyme, pH 8.0

659815

0.0746

acetyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme M564G

674651

0.076

acetyl-CoA

Saccharomyces cerevisiae

-

-

487418

0.078

acetyl-CoA

Homo sapiens

-

peroxisomal enzyme, at pH 7.4 and 37°C

735697

0.087

acetyl-CoA

Homo sapiens

P43155

Y431A mutant enzyme, pH 8.0

659815

0.0921

acetyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme S554A

674651

0.12

acetyl-CoA

Homo sapiens

P43155

R497Q mutant enzyme, pH 8.0

659815

0.13

acetyl-CoA

Candida tropicalis

-

-

487405

0.178

acetyl-CoA

Homo sapiens

P43155

F545A mutant enzyme, pH 8.0

659815

0.188

acetyl-CoA

Homo sapiens

P43155

T444A mutant enzyme, pH 8.0

659815

0.24

acetyl-CoA

Homo sapiens

-

mitochondrial enzyme, at pH 7.4 and 37°C

735697

0.498

acetyl-CoA

Homo sapiens

P43155

Y431F mutant enzyme, pH 8.0

659815

0.019

acetylcarnitine

Rattus norvegicus

-

supplementation with R-alpha-lipoic acid

487432

0.28

acetylcarnitine

Rattus norvegicus

-

-

487403

0.39

acetylcarnitine

Rattus norvegicus

-

enzyme from liver homogenate

487408

0.417

acetylcarnitine

Candida tropicalis

-

peroxisomal enzyme

487406

0.42

acetylcarnitine

Homo sapiens

-

-

487416

0.6

acetylcarnitine

Rattus norvegicus

-

enzyme from mitochondria

487408

0.639

acetylcarnitine

Candida tropicalis

-

mitochondrial enzyme

487406

0.015

butyryl-CoA

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast, pH 7.8, 30°C

659372

0.0166

butyryl-CoA

Mus musculus

-

-

487423

0.029

butyryl-CoA

Camelus dromedarius

-

-

487423

0.03

butyryl-CoA

Rattus norvegicus

Q704S8

recombinant enzyme from yeast, pH 7.8, 30°C

659372

0.038

butyryl-CoA

Rattus norvegicus

-

-

487403

0.0435

butyryl-CoA

Homo sapiens

-

-

487416, 487423

0.044

butyryl-CoA

Columba sp.

-

-

487423

0.499

butyryl-CoA

Homo sapiens

-

mitochondrial enzyme, at pH 7.4 and 37°C

735697

0.48

butyrylcarnitine

Rattus norvegicus

-

enzyme from liver homogenate

487408

0.66

butyrylcarnitine

Rattus norvegicus

-

enzyme from mitochondria

487408

0.0035

carnitine

Mus musculus

P47934

liver homogenate, pH 8.0, temperature not specified in the publication

756008

0.011

carnitine

Mus musculus

P47934

mitochondria from liver, pH 8.0, temperature not specified in the publication

756008

0.0537

carnitine

Mus musculus

P47934

heart homogenate, pH 8.0, temperature not specified in the publication

756008

0.0562

carnitine

Mus musculus

P47934

mitochondria from heart, pH 8.0, temperature not specified in the publication

756008

0.086

carnitine

Mus musculus

-

-

486295

0.086

carnitine

Homo sapiens

-

cosubstrate propionyl-CoA

487416

0.086

carnitine

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast with octanoyl-CoA as second substrate, pH 7.8, 30°C

659372

0.097

carnitine

Homo sapiens

-

cosubstrate acetyl-CoA

487416

0.101

carnitine

Rattus norvegicus

-

wild-type enzyme

672172

0.12

carnitine

Homo sapiens

-

cosubstrate hexanoyl-CoA

487416

0.148

carnitine

Homo sapiens

-

cosubstrate octanoyl-CoA

487416

0.152

carnitine

Homo sapiens

-

cosubstrate butyryl-CoA

487416

0.164

carnitine

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast with hexanoyl-CoA as second substrate, pH 7.8, 30°C

659372

0.17

carnitine

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast with butyryl-CoA as second substrate, pH 7.8, 30°C

659372

0.203

carnitine

Rattus norvegicus

Q704S8

recombinant enzyme from yeast with acetyl-CoA as second substrate, pH 7.8, 30°C

659372

0.22

carnitine

Rattus norvegicus

Q704S8

recombinant enzyme from yeast with butyryl-CoA as second substrate, pH 7.8, 30°C

659372

0.339

carnitine

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast with acetyl-CoA as second substrate, pH 7.8, 30°C

659372

0.395

carnitine

Columba livia

-

-

704846

0.4

carnitine

Columba sp.

-

-

487399

0.567

carnitine

Rattus norvegicus

Q704S8

recombinant enzyme from yeast with hexanoyl-CoA as second substrate, pH 7.8, 30°C

659372

0.58

carnitine

Homo sapiens

-

cosubstrate decanoyl-CoA

487416

0.964

carnitine

Rattus norvegicus

Q704S8

recombinant enzyme from yeast with octanoyl-CoA as second substrate, pH 7.8, 30°C

659372

1

carnitine

Aspergillus niger

-

mitochondrial enzyme

487424

1.1

carnitine

Aspergillus niger

-

cytosolic enzyme

487424

260

carnitine

Rattus norvegicus

-

mutant enzyme T465V/T467N/R518N

672172

300

carnitine

Rattus norvegicus

-

above, mutant enzyme A106M/T465V/T467N/R518N

672172

1.1

choline

Oryctolagus cuniculus

-

brain enzyme

487399

5

choline

Oryctolagus cuniculus

-

heart enzyme

487399

8.7

choline

Columba sp.

-

-

487399

18.1

choline

Rattus norvegicus

-

mutant enzyme A106M/T465V/T467N/R518N

672172

29

choline

Rattus norvegicus

-

mutant enzyme T465V/T467N/R518N

672172

86.4

choline

Rattus norvegicus

-

wild-type enzyme

672172

0.0328

CoA

Mus musculus

P47934

mitochondria from heart, pH 8.0, temperature not specified in the publication

756008

0.0664

CoA

Mus musculus

P47934

heart homogenate, pH 8.0, temperature not specified in the publication

756008

0.0888

CoA

Mus musculus

P47934

mitochondria from liver, pH 8.0, temperature not specified in the publication

756008

0.1134

CoA

Mus musculus

P47934

liver homogenate, pH 8.0, temperature not specified in the publication

756008

0.009

CoASH

Bos taurus

-

-

487407

0.0153

CoASH

Mus musculus

-

-

486295, 487423

0.018

CoASH

Rattus norvegicus

-

supplementation with R-alpha-lipoic acid

487432

0.022

CoASH

Rattus norvegicus

-

substrate hexanoylcarnitine, enzyme from liver homogenate

487408

0.024

CoASH

Rattus norvegicus

-

substrate decanoylcarnitine, enzyme from liver homogenate

487408

0.027

CoASH

Rattus norvegicus

-

substrate decanoylcarnitine, enzyme from mitochondria

487408

0.027

CoASH

Rattus norvegicus

-

substrate butyrylcarnitine and octanoylcarnitine, enzyme from liver homogenate

487408

0.0277

CoASH

Camelus dromedarius

-

-

487423

0.028

CoASH

Rattus norvegicus

-

substrate acetylcarnitine, enzyme from liver homogenate

487408

0.03

CoASH

Rattus norvegicus

-

-

487403

0.032

CoASH

Rattus norvegicus

-

-

487423

0.032

CoASH

Rattus norvegicus

-

substrate acetylcarnitine, enzyme from mitochondria

487408

0.034

CoASH

Columba sp.

-

-

487404, 487423

0.037

CoASH

Columba sp.

-

-

487403

0.038

CoASH

Rattus norvegicus

-

substrate butyrylcarnitine, enzyme from mitochondria

487408

0.041

CoASH

Sus scrofa

-

-

487398

0.044

CoASH

Rattus norvegicus

-

substrate hexanoylcarnitine, enzyme from mitochondria

487408

0.054

CoASH

Rattus norvegicus

-

substrate octanoylcarnitine, enzyme from mitochondria

487408

0.069

CoASH

Rattus norvegicus

-

-

487404

0.09

CoASH

Aspergillus niger

-

mitochondrial enzyme

487424

0.1

CoASH

Aspergillus niger

-

-

487424

0.11

CoASH

Aspergillus niger

-

cytosolic enzyme

487424

0.257

CoASH

Candida tropicalis

-

mitochondrial enzyme

487406

0.304

CoASH

Candida tropicalis

-

peroxisomal enzyme

487406

2

CoASH

Rattus norvegicus

-

-

487432

0.0287

decanoyl-CoA

Mus musculus

-

-

486295

0.064

decanoyl-CoA

Homo sapiens

-

-

487416

0.64

decanoylcarnitine

Rattus norvegicus

-

enzyme from mitochondria

487408

0.66

decanoylcarnitine

Rattus norvegicus

-

enzyme from liver homogenate

487408

0.72

DL-carnitine

Rattus norvegicus

-

-

487403

3.3

DL-carnitine

Candida tropicalis

-

-

487405

0.02

hexanoyl-CoA

Mus musculus

-

-

486295

0.022

hexanoyl-CoA

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast, pH 7.8, 30°C

659372

0.0224

hexanoyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme M564G

674651

0.033

hexanoyl-CoA

Rattus norvegicus

Q704S8

recombinant enzyme from yeast, pH 7.8, 30°C

659372

0.0415

hexanoyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme S554A

674651

0.0549

hexanoyl-CoA

Homo sapiens

-

-

487416

0.1034

hexanoyl-CoA

Mus musculus

P47934

21°C, pH 7.8, wild-type enzyme

674651

0.112

hexanoyl-CoA

Mus musculus

P47934

21°C, pH 7.8, mutant enzyme S554A/M564G

674651

2.28

hexanoylcarnitine

Rattus norvegicus

-

enzyme from liver homogenate

487408

2.51

hexanoylcarnitine

Rattus norvegicus

-

enzyme from mitochondria

487408

0.039

L-(-)-carnitine

Camelus dromedarius

-

-

487422

0.12

L-(-)-carnitine

Bos taurus

-

-

487407

0.12

L-(-)-carnitine

Columba sp.

-

-

487403, 487404

0.15

L-(-)-carnitine

Rattus norvegicus

-

-

487404

0.17

L-(-)-carnitine

Saccharomyces cerevisiae

-

-

487418

0.23

L-(-)-carnitine

Saccharomyces cerevisiae

-

-

487418

0.244

L-(-)-carnitine

Camelus dromedarius

-

-

487422

0.1

L-carnitine

Cavia porcellus

-

at pH 7.8 and 37°C

736607

0.1

L-carnitine

Rattus norvegicus

-

at pH 7.8 and 37°C

736607

0.11

L-carnitine

Homo sapiens

P43155

wild type enzyme, pH 8.0

659815

0.121

L-carnitine

Mus musculus

-

cosubstrate butanoyl-CoA

486295

0.15

L-carnitine

Homo sapiens

P43155

F545Y mutant enzyme, pH 8.0

659815

0.344

L-carnitine

Mus musculus

-

cosubstrate hexanoyl-CoA

486295

0.395

L-carnitine

Mus musculus

-

cosubstrate octanoyl-CoA

486295

0.519

L-carnitine

Mus musculus

-

cosubstrate decanoyl-CoA

486295

0.622

L-carnitine

Candida tropicalis

-

mitochondrial enzyme, second substrate acetyl-CoA

487406

0.655

L-carnitine

Candida tropicalis

-

peroxisomal enzyme, second substrate propionyl-CoA

487406

0.719

L-carnitine

Candida tropicalis

-

peroxisomal enzyme, second substrate acetyl-CoA

487406

0.774

L-carnitine

Candida tropicalis

-

mitochondrial enzyme, second substrate propionyl-CoA

487406

2

L-carnitine

Homo sapiens

P43155

F545A mutant enzyme, pH 8.0

659815

7.5

L-carnitine

Homo sapiens

P43155

T444A mutant enzyme, pH 8.0

659815

11.1

L-carnitine

Homo sapiens

P43155

Y431A mutant enzyme, pH 8.0

659815

25.6

L-carnitine

Homo sapiens

P43155

R497Q mutant enzyme, pH 8.0

659815

35.9

L-carnitine

Homo sapiens

P43155

Y431F mutant enzyme, pH 8.0

659815

0.3

O-acetyl-L-carnitine

Bos taurus

-

heart enzyme

487407

-

0.36

O-acetyl-L-carnitine

Bos taurus

-

sperm enzyme

487407

-

0.011

octanoyl-CoA

Rattus norvegicus

Q704S8

M564G mutant enzyme from yeast, pH 7.8, 30°C

659372

0.0191

octanoyl-CoA

Mus musculus

-

-

486295

0.025

octanoyl-CoA

Rattus norvegicus

Q704S8

recombinant enzyme from yeast, pH 7.8, 30°C

659372

0.0503

octanoyl-CoA

Homo sapiens

-

-

487416

1.27

octanoylcarnitine

Rattus norvegicus

-

enzyme from mitochondria

487408

1.39

octanoylcarnitine

Homo sapiens

-

-

487416

1.62

octanoylcarnitine

Rattus norvegicus

-

enzyme from liver homogenate

487408

0.014

propionyl-CoA

Bos taurus

-

-

487407

0.0173

propionyl-CoA

Camelus dromedarius

-

-

487423

0.028

propionyl-CoA

Homo sapiens

-

-

487416, 487423, 487427

0.034

propionyl-CoA

Candida albicans

-

peroxisomal enzyme

487427

0.037

propionyl-CoA

Columba sp.

-

-

487423

0.1

propionyl-CoA

Candida tropicalis

-

peroxisomal enzyme

487406, 487427

0.162

propionyl-CoA

Candida tropicalis

-

mitochondrial enzyme

487406, 487427

0.185

propionyl-CoA

Candida albicans

-

mitochondrial enzyme

487427