Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.65: bile acid-CoA:amino acid N-acyltransferase

This is an abbreviated version!
For detailed information about bile acid-CoA:amino acid N-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.65

Reaction

choloyl-CoA
+
glycine
=
CoA
 +
glycocholate

Synonyms

acyltransferase, glycine-taurine N-, amino acid N-choloyltransferase, BAAT, BAATP1, BACAT, BAT, bile acid CoA: amino acid N-acyltransferase, bile acid CoA:amino acid N-acyltransferase, bile acid CoA:N-acyltransferase, bile acid coenzyme A-amino acid N-acyltransferase, bile acid coenzyme A: amino acid N-acyltransferase, bile acid coenzyme A:amino acid N-acyltransferase, bile acid-CoA:amino acid N acyltransferase, bile acid-CoA:amino acid N-acyltransferase, bile acid–coenzyme A:amino acid N-acyltransferase, glycine-taurine N-acyltransferase, hBAT, mBAT, rBAT

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.65 bile acid-CoA:amino acid N-acyltransferase

Cloned

Cloned on EC 2.3.1.65 - bile acid-CoA:amino acid N-acyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA encoding enzyme is cloned, sequenced and expressed in Escherichia coli DH5alpha, the open-reading frame codes for a 420 amino acid protein, Baat gene is mapped to mouse chromosome 4, single gene encodes enzyme
-
expressed in Escherichia coli DH5alpha cells
-
expression in Escherichia coli
expression in Sf9 cells
-
expression in Sf9 cells. Initial attempts to express rBAT in Escherichia coli with vectors such as pMAL-p2 and pKK233-2 are unsuccessful due to proteolytic degradation of the expressed protein
-
expression of GFP-tagged enzyme in HeLa cells, which is exclusively located in the cytosol, high mobility of the GFP-fusion proteins in the cytosol and absence of peroxisomal escape
-
expression of wild-type and mutant enzymes
-
gene/full-length cDNA is cloned and expressed in Escherichia coli XL1-Blue or BL21
-
when expressed in fibroblasts, GFP-tagged hBAAT localizes to the cytosol
-