2.3.1.64: agmatine N4-coumaroyltransferase

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For detailed information about agmatine N4-coumaroyltransferase, go to the full flat file.

Reaction

Synonyms

ACT, agmatine coumaroyltransferase, agmatine coumaryl transferase, At5g61160 protein, AtACT, coumaroyltransferase, agmatine, p-coumaroyl-CoA-agmatine N-p-coumaroyltransferase

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.64 agmatine N4-coumaroyltransferase

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Results	in table
2	Activating Compound
12	AA Sequence
1	Application
1	CAS Registry Number
5	Cloned(Commentary)
2	Expression
2	General Information
1	General Stability
8	Inhibitors
24	KM Value [mM]
1	Localization
5	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
9	Organism
3	Pathway
1	PDB ID
4	pH Optimum
3	pH Range
1	Protein Variants
4	Purification (Commentary)
1	Reaction
1	Reaction Type
9	Reference
6	Source Tissue
12	Specific Activity [micromol/min/mg]
2	Storage Stability
20	Substrates and Products (Substrate)
1	Subunits
13	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Range [°C]

Purification

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Purification on EC 2.3.1.64 - agmatine N4-coumaroyltransferase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

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Hordeum vulgare

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487362

ammonium sulfate, Biogel A, Sepharose 4B-agmatine

Hordeum vulgare

-

487361

Blue Sepharose, t-butyl-Sepharose, Resource Q, Superose 12 HR

Hordeum vulgare

A9ZPJ6

487363

frozen leaves are homogenized in 100 mM Tris buffer, pH 7.5, containing 10 mM 2-mercaptoethanol and 5 mM DTT, centrifugation, supernatant applied to Sephadex G-50 column, fluent used for enzyme assays, or recombinant Escherichia coli cells are harvested and resuspended in 100 mM Tris buffer, pH 7.5, containing 1 mM EDTA, 50 mM NaCl, 10% glycerol, lysed with lysozyme, sonicated, centrifuged, supernatant used for enzyme assays and SDS-PAGE

Arabidopsis thaliana

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706386