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2.3.1.54: formate C-acetyltransferase

This is an abbreviated version!
For detailed information about formate C-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.54

Reaction

acetyl-CoA
+
formate
=
CoA
 +
pyruvate

Synonyms

AF_1449, formate acetyltransferase, PFL, PFL I, Pfl1, PflB, phosphotransferase system enzyme I, pyruvate formate lyase, pyruvate formate-lyase, pyruvate-formate lyase, pyruvate:formate lyase, pyruvic formate-lyase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.54 formate C-acetyltransferase

Purification

Purification on EC 2.3.1.54 - formate C-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
centrifugation, washing in 50 mM potassium phosphate buffer, pH 7.5, resuspension, French press disruption, centrifugation of crude extract and of resulting supernatant
-
gel filtration, Western Blot, heterologously expressed protein
native enzyme at pH 6.4, by anion exchange and hydrophobic interaction chromatography, and gel filtration, to homogeneity
-
native enzyme from strain BL21(DE3) by gel filtration and anion exchange chromatography, recombinant His6-tagged enzyme from strain BL21(DE3) by nickel affinity chromatography as single enzyme or as AdhE-Pfl
-
partially
-
quaternary methylamine AP5-ion exchange column chromatography and phenyl Sepharose column chromatography
-
recombinant enzyme
recombinant His-tagged wild-type and mutant enzymes from strain BL21(DE3) by nickel affinity chromatography to homogeneity
-