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2.3.1.54: formate C-acetyltransferase

This is an abbreviated version!
For detailed information about formate C-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.54

Reaction

acetyl-CoA
+
formate
=
CoA
 +
pyruvate

Synonyms

AF_1449, formate acetyltransferase, PFL, PFL I, Pfl1, PflB, phosphotransferase system enzyme I, pyruvate formate lyase, pyruvate formate-lyase, pyruvate-formate lyase, pyruvate:formate lyase, pyruvic formate-lyase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.54 formate C-acetyltransferase

Crystallization

Crystallization on EC 2.3.1.54 - formate C-acetyltransferase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified enzyme, sitting drop vapour diffusion method, mixing 0.001 ml of 20 mg/ml protein solution with 0.001 ml precipitant solution containing 16% w/v PEG 8000, 8% v/v isopropanol, 80 mM Hepes, pH 7.5, 160 mM ammonium sulfate and 1 mM DTT, plate-like crystals, X-ray diffraction structure determination and analysis at 2.9 A resolution
-
three-dimensional modeling of the corresponding protein and molecular dynamic simulations, predict an alkylsuccinate synthase activity. The four stereoisomers of (1-methyl-pentadecyl)succinate formed by addition of the sub-terminal carbon atom (C-2) of hexadecane to fumarate are able to exit the protein tunnel
space group P4(1)2(1)2
-
space group P4(3)2(1)2 a = b = 159 A, c = 160 A
space group P4(3)2(1)2, cell parameters a = b = 158.36 A, c = 159.30 A
structure of the pyruvate formate-lyase monomer in complex with pyruvate and CoA, structure of active site, small model computational studies of the pyruvate formate-lyase substrate transformation
tetragonal crystals in complex with pyruvate, monoclinic cocrystals with CoA and either pyruvate or oxamate obtained by hanging drop method, space group C222_1, a = 54.90 A, b = 153.05 A, c = 205.95