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2.3.1.50: serine C-palmitoyltransferase

This is an abbreviated version!
For detailed information about serine C-palmitoyltransferase, go to the full flat file.

Word Map on EC 2.3.1.50

Reaction

palmitoyl-CoA
+
L-serine
=
CoA
 +
3-dehydro-D-sphinganine
 +
CO2

Synonyms

3-oxosphinganine synthetase, acyl-CoA:serine C-2 acyltransferase decarboxylating, LCB1, LCB2, LCB2a, LCB2b, More, palmitoyltransferase, serine, serine palmitoyl transferase, serine palmitoyltransferase, serine palmitoyltransferase 1, serine palmitoyltransferase a, serine-palmitoyl transferase, serine-palmitoyltransferase, SPT, SPT1, SPT2, SPT3, SPTase, SPTLC1, SPTLC2, ssSPT, ssSPTa, Tsc3

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.50 serine C-palmitoyltransferase

Cloned

Cloned on EC 2.3.1.50 - serine C-palmitoyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as a His-tagged fusion protein in Escherichia coli
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21-Gold (DE3) cells
expressed in HEK293T cells
expressed in Saccharomyces cerevisiae DELTAlcb1 strain
expressed in yeast and mammalian cells, a triple fusion protein of the three subunits expressed in yeast and mammalian cells
-
expression in Escherichia coli
-
expression in HEK 293 cells
expression of FLAG-tagged LCB1 in the deficient mutant LY-B, overproduction of LCB2 requires co-overproduction of LCB1
-
expression of HA-tagged C133W mutant SPTLC1 in CHO-K1 cells and in transgenic mice, the expression dominantly inhibits SPT activity
-
expression of mLCB1 and LCB2 as glutathione-S-transferase fusion protein in Escherichia coli DH5alpha
-
expression of wild-type and mutant enzymes in HEK-293 cells. The overexpression of wild-type subunit SPTLC1 does not increase SPT enzyme activity compared with controls (empty vector), whereas overexpressing subunit SPTLC2 results in a 2fold increase in enzyme activity
expression of wild-type and mutant smalll subunits isoforms of serine palmitoyltransferase in yeast microsomes
-
gene ehv050, DNA and amino acid sequence determination and analysis, functional expression of HA-tagged enzyme in Saccharmoyces cerevisiae endoplasmic reticulum, when the two subunits of the yeast SPT are thus expressed, the single-chain chimera is functional and displays a different substrate preference, independently expressed domains of the viral SPT reassemble into an active protein, overview
-
genes LCB1 and LCB2, overexpression
-
His-tagged protein expressed in Escherichia coli BL21(DE3)
hLCB1 and hLCB2
-
in vitro transcription and translation of mLCB2
-
individual overexpression of subunits SPTLC1, SPTLC2, and SPTLC3, fused to a C-terminal V5-his tag, in HEK-293 cells
-
LCB2 cloning by ESTs, DNA and amino acid sequence determination and analysis, LCB2 expression analysis and phylogenetic analysis, overexpression of subunit LCB2 in Saccharomyces cerevisiae strain W303, transient overexpression in Nicotiana tabacum leaves via Agrobacterium tumefaciens strain GV2260-mediated transfection. BcLCB2 encodes a bona fide LCB2 subunit and the SPT activity in yeast results from co-expression of the BcLCB1 and BcLCB2 subunits
overexpression in Escherichia coli BL21 (DE3) pLysS, DNA sequence determination
overexpression of mLCB1 and mLCB2 in human embryonic kidney cells, independently and in coexpression with mLCB1
-
overexpression of SPTLC3 in HEK-293 cells leads to the formation of two new sphingoid base metabolites, namely C16-sphinganine and C16-sphingosine. SPTLC3-expressing cells have higher in vitro SPT activities with lauryl- and myristoyl-CoA than SPTLC2-expressing cells, and SPTLC3 mRNA expression levels correlate closely with the C16-sphinganine synthesis rates in various human and murine cell lines. Quantitative reverse transcription--PCR analysis
-
overexpression of subunit SPTLC3 in HEK-293 cells, leading to an 2-3fold increased SPT activity, expression of subunit SPTLC3 in Hep-G2 cells and human trophoblast cells, quantitative expression analysis of SPTLC3
-
recombinant expression of FLAG-tagged enzyme subunit LCB1 and of Myc-tagged enzyme subunit LCB2 in Saccharomyces cerevisiae microsomes. Expressing the Arabidopsis thaliana LCB1/LCB2 heterodimers has very low SPT activity, whereas coexpression of Arabidopsis thaliana ssSPTs, ssSPTa and ssSPTb, derived 56-amino acid polypeptides in a Saccharomyces cerevisiae enzyme SPT null mutant and stimulate SPT enzyme activity of the Arabidopsis LCB1/LCB2 heterodimer by over 100fold through physical interaction with LCB1/LCB2. Transient Agrobacterium tumefaciens-mediated transformation of Nicotiana benthaminana with YFP-labeled small subunits of serine palmitoyltransferase, ssSPTa and ssSPTb
-
recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
recombinant expression of ssSPTa, tagged with EGFP at its N-terminus, in HEK-293 cells
recombinant expression of subunit LCB2a mutants in HEK293 cells, recombinant His-tagged enzyme expression in Escherichia coli strain BL21(DE3). Recombinant expression of wild-type hLCB1/hLCB2a subunits in Saccharomyces cerevisiae microsomes in the absence of either small subunit, the enzyme shows very low activity. Coexpression of each of the three subunit hLCB2a mutants, V359M, G382V, and I504F, along with subunit hLCB1 results in low activity, with G382V barely detectable above background
recombinant stable expression of mutant and wild-type SPTLC2 subunit in HEK293 cells
RT-PCR from mRNA of Molt-4 cells
SPT expression in Escherichia coli strain BL21(DE3)
SPT1 and SPT2 real-time PCR expression analysis
-
ssSPTa and ssSPTb, DNA and amino acid sequence determination and analysis. Transient functional overexpression of ssSPTb-containing isozymes in CHO LyB cells increasing C20-long-chain bases. Expression of the human enzyme subunits in the lcb1DELTAlcb2DELTA double mutant Saccharomyces cerevisiae cells in various combinations constituting enzyme activity, overview. Expression in CHO LyB cells lacking endogenous SPT activity
-
stable expression of LCB1 and R246G mutant SPT in enzyme-deficient hamster LY-B ovary cells restores SPT activity, DNA and amino acid sequence determination and analysis of the recombinantly expressed R246G mutant enzyme
-
subunit 2 expressed in HEK293 cells
subunit LCB1, DNA and amino acid sequence determination and analysis
subunits LCB1 and LCB2 expression analysis
subunits LCB1 and LCB2, phylogenetic tree, expression of Arabidopsis thaliana subunit LCB1 rescues the sphingolipid long-chain base auxotrophy of Saccharomyces cerevisiae SPT mutants when coexpressed with Arabidopsis thaliana LCB2