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2.3.1.5: arylamine N-acetyltransferase

This is an abbreviated version!
For detailed information about arylamine N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.5

Reaction

acetyl-CoA
+
an arylamine
=
CoA
+
an N-acetylarylamine

Synonyms

(BACAN)NAT3, (MYCAB)NAT1, 2-naphthylamine N-acetyltransferase, 4-aminobiphenyl N-acetyltransferase, ABW01_24350, acetyl CoA-arylamine N-acetyltransferase, acetyltransferase, 2-naphthylamine N-, acetyltransferase, 4-aminobiphenyl, acetyltransferase, arylamine, acetyltransferase, p-aminosalicylate N-, acetyltransferase, procainamide N-, acetyltransferase, serotonin N-, arylamine acetylase, arylamine acetyltransferase, arylamine N-acetyl transferase, arylamine N-acetyltransferase, arylamine N-acetyltransferase 1, arylamine N-acetyltransferase 2, arylamine N-acetyltransferase C, arylamine N-acetyltransferase I, arylamine N-acetyltransferase type 1, arylamine N-acetyltransferase type 2, arylamine N-acetyltransferase type I, arylamine-N-acetyltransferase 1, BanatA, BanatB, BanatC, beta-naphthylamine N-acetyltransferase, indoleamine N-acetyltransferase, MlNAT1, MMNAT, More, MSNAT, N-acetyltransferase, N-acetyltransferase a, N-acetyltransferase b, N-acetyltransferase type 2, N-hydroxyarylamine O-acetyltransferase, NAT, NAT 1, NAT-a, NAT-b, NAT1, NAT2, NAT2*1, NAT2*2, NAT3, NAT31, NfNAT, p-aminosalicylate N-acetyltransferase, PANAT, rhesus NAT2, serotonin acetyltransferase, serotonin N-acetyltransferase, STNAT, TBNAT, Tpau_4046, UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase, UDP-D-Glc(2NAc3N)A 3-N-acetyltransferase, WbpD

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.5 arylamine N-acetyltransferase

Purification

Purification on EC 2.3.1.5 - arylamine N-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography
-
ammonium sulfate precipitation
-
by using chromatographic purification. Yield: 2.8 mg of homogeneous NAT2 from 2 L of cell culture
-
cDNA-isolation
-
cobalt Talon column chromatography and Ni-NTA resin column chromatography
His-select nickel resin column chromatography
-
monoclonal antibodies
-
NAT is purified from the extract of Bacillus cereus cultures using ammonium sulfate precipitation, a DEAE-cellulose, a DEAE-Toyopearl and a hydrophobic POROS 20PE column
-
native enzyme partially by subcellular fractionation
Ni-NTA affinity column chromatography, and gel filtration
-
Ni-NTA column chromatography
Ni-NTA column chromatography and Mono Q column chromatography
-
Ni-NTA resin column chromatography, and gel filtration
nickel ion affinity chromatography
-
partial
partial, 3 isozymes from 2 strains
-
proteins are purified from tissue homogenates
-
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged isozyme NAT1 from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally His-tagged enzyme from strain CQW40 to over 95% purity by immobilized metal affinity chromatography
-
recombinant N-terminally His6-tagged NAT from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein, fusion with dihydrofolate reductase
-
recombinant shNAT2
-
recombinant wild-type and mutant enzymes NAT2 from HEK-293T cells by affinity chromatography
recombinant wild-type and mutant NAT1 from Escherichia coli
recombinant wild-type and mutant NAT2 from Escherichia coli by anion exchange chromatography
using a HIS-Select nickel resin, CM Sephadex ion exchange chromatography
A0A0F7R932, Q81PT0, Q81R98
using a Ni-NTA and a Superdex S-75 column
-
using a Ni-NTA resin
-
using DE52 column chromatography, DEAE-Toypearl columns and hydrophobic POROS 20PE columns
-
using HIS-select nickel resin
-
using HIS-select nickel resin and CM Sephadex ion exchange chromatography, in addition protein extracts from stationary phase cultures of Bacillus anthracis are preapred
A0A0F7R932, Q81PT0, Q81R98
using HIS-Select resin
using Ni-NTA agarose, the hexa-his-tag is removed by thrombin cleavage
-
using Ni-NTA chromatography
using Ni-NTA chromatography. Approximately 2.7 mg of purified enzyme is obtained per liter of culture
-
using Ni-NTA chromatography. Protein yield: approximately 30 mg
-
using Ni2+-agarose
-
using using Ni-NTA agarose
-