2.3.1.5: arylamine N-acetyltransferase
This is an abbreviated version!
For detailed information about arylamine N-acetyltransferase, go to the full flat file.
Word Map on EC 2.3.1.5
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2.3.1.5
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pineal
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melatonin
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rhythm
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circadian
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n-acetylation
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retina
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2-aminofluorene
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night
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p-aminobenzoic
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nocturnal
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arylalkylamine
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n-acetylserotonin
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hydrazine
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medicine
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diurnal
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isoniazid
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light-dark
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aa-nat
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paba
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pinealocytes
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nighttime
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hydroxyindole-o-methyltransferase
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sulfamethazine
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hiomt
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accoa
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xenobiotic-metabolizing
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monomorphic
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daytime
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quinpirole
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tryptamine
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anti-tubercular
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pharmacology
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sulpiride
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light-exposed
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analysis
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molecular biology
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n-hydroxylated
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drug development
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midnight
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p-aminosalicylic
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o-acetyltransferase
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n-hydroxyarylamine
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spiroperidol
- 2.3.1.5
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pineal
- melatonin
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rhythm
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circadian
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n-acetylation
- retina
- 2-aminofluorene
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night
-
p-aminobenzoic
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nocturnal
- arylalkylamine
- n-acetylserotonin
- hydrazine
- medicine
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diurnal
- isoniazid
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light-dark
- aa-nat
- paba
- pinealocytes
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nighttime
- hydroxyindole-o-methyltransferase
- sulfamethazine
- hiomt
- accoa
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xenobiotic-metabolizing
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monomorphic
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daytime
- quinpirole
- tryptamine
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anti-tubercular
- pharmacology
- sulpiride
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light-exposed
- analysis
- molecular biology
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n-hydroxylated
- drug development
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midnight
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p-aminosalicylic
- o-acetyltransferase
- n-hydroxyarylamine
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spiroperidol
Reaction
Synonyms
(BACAN)NAT3, (MYCAB)NAT1, 2-naphthylamine N-acetyltransferase, 4-aminobiphenyl N-acetyltransferase, ABW01_24350, acetyl CoA-arylamine N-acetyltransferase, acetyltransferase, 2-naphthylamine N-, acetyltransferase, 4-aminobiphenyl, acetyltransferase, arylamine, acetyltransferase, p-aminosalicylate N-, acetyltransferase, procainamide N-, acetyltransferase, serotonin N-, arylamine acetylase, arylamine acetyltransferase, arylamine N-acetyl transferase, arylamine N-acetyltransferase, arylamine N-acetyltransferase 1, arylamine N-acetyltransferase 2, arylamine N-acetyltransferase C, arylamine N-acetyltransferase I, arylamine N-acetyltransferase type 1, arylamine N-acetyltransferase type 2, arylamine N-acetyltransferase type I, arylamine-N-acetyltransferase 1, BanatA, BanatB, BanatC, beta-naphthylamine N-acetyltransferase, indoleamine N-acetyltransferase, MlNAT1, MMNAT, More, MSNAT, N-acetyltransferase, N-acetyltransferase a, N-acetyltransferase b, N-acetyltransferase type 2, N-hydroxyarylamine O-acetyltransferase, NAT, NAT 1, NAT-a, NAT-b, NAT1, NAT2, NAT2*1, NAT2*2, NAT3, NAT31, NfNAT, p-aminosalicylate N-acetyltransferase, PANAT, rhesus NAT2, serotonin acetyltransferase, serotonin N-acetyltransferase, STNAT, TBNAT, Tpau_4046, UDP-2-acetamido-3-amino-2,3-dideoxy-D-glucuronic acid 3-N-acetyltransferase, UDP-D-Glc(2NAc3N)A 3-N-acetyltransferase, WbpD
ECTree
2.3.1.5 arylamine N-acetyltransferaseAdvanced search results
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results (KM Value
KM Value on EC 2.3.1.5 - arylamine N-acetyltransferase
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KM VALUE [mM] 
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
2.6
3-amino-4-hydroxybenzaldehyde
pH 7.0, 30°C, recombinant enzyme
12
3-amino-4-hydroxybenzenesulfonic acid
pH 7.0, 30°C, recombinant enzyme
0.048
4,4'-methylenebis(2-chloroaniline)
variant NAT2 4, pH 7.4, temperature not specified in the publication
0.486
malonyl-CoA
cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication
0.00222
2-Aminofluorene
Hep-G2 cells, presence of alpha-solanine, pH 7.5, 37°C
0.00948
2-Aminofluorene
cytosol preparation, presence of alpha-solanine, pH 7.5, 37°C
0.0165
2-Aminofluorene
artificial full-length enzyme, pH 7.5, 37°C
0.311
2-Aminofluorene
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strain 60.62, pH 7.4, 37°C
0.365
2-Aminofluorene
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strain YG1024, pH 7.4, 37°C
0.397
2-Aminofluorene
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strain TA98, pH 7.4, 37°C
0.404
2-Aminofluorene
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strain TA1538, pH 7.4, 37°C
0.86
2-Aminofluorene
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mutant enzyme E123D, apparent value, at pH 7.5 and 25°C
1.1
2-toluidine
pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate
1.2
2-toluidine
pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate
6.6
2-toluidine
pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate
0.17
4-amino-3-methylbenzoic acid
pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate
0.18
4-amino-3-methylbenzoic acid
pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate
11
4-amino-3-methylbenzoic acid
pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate
0.052
4-Aminobenzoate
presence of 1 mM ATP, 37°C, pH not specified in the publication
0.049
4-Aminobenzoic acid
pH 7.0, 23°C, isozyme NAT1, with 4-nitrophenyl acetate
0.061
4-Aminobenzoic acid
pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate
0.23
4-Aminobenzoic acid
pH 7.0, 25°C, recombinant wild-type enzyme
0.37
4-Aminobenzoic acid
pH 7.0, 25°C, recombinant mutant R64W
166
4-Aminobenzoic acid
pH 7.0, 23°C, isozyme NAT2, with 4-nitrophenyl acetate
0.189
4-Aminosalicylate
artificial full-length enzyme, pH 7.5, 37°C
0.64
4-aminosalicylic acid
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mutant enzyme E123D, apparent value, at pH 7.5 and 25°C
1.34
4-aminosalicylic acid
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wild type enzyme, apparent value, at pH 7.5 and 25°C
1.8
4-anisidine
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
1.8
4-anisidine
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
2.3
4-anisidine
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
2.3
4-anisidine
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
4.81
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant mutant R64W
10
4-nitrophenyl acetate
pH 7.0, 25°C, recombinant wild-type enzyme
0.208
5-aminosalicylic acid
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strain 60.62, pH 7.4, 37°C
1.2
5-aminosalicylic acid
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
1.2
5-aminosalicylic acid
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
1.2
5-aminosalicylic acid
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
1.2
5-aminosalicylic acid
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
0.05
acetyl-CoA
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wild type enzyme, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C
0.052
acetyl-CoA
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mutant enzyme E123D, apparent value, with 4-aminosalicylic acid as cosubstrate, at pH 7.5 and 25°C
0.07
acetyl-CoA
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
0.07
acetyl-CoA
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
0.136
acetyl-CoA
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concentration p-anisidine: 200 nM, reaction: acetyl-CoA + p-anisidine
0.181
acetyl-CoA
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
0.181
acetyl-CoA
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
0.466
acetyl-CoA
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concentration p-anisidine: 2 mM, reaction: acetyl-CoA + p-anisidine
0.651
acetyl-CoA
presence of 1 mM ATP, 37°C, pH not specified in the publication
2.222
acetyl-CoA
cosubstrate 5-aminosalicylate, pH 7.5, temperature not specified in the publication
4.7
isoniazid
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
4.7
isoniazid
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chimeric enzyme, replacement of the third domain of Mycobacterium tuberculosis enzyme with that from Mycobacterium marinum, pH 8.0, 28°C
6.1
isoniazid
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
6.1
isoniazid
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chimeric enzyme, replacement of the third domain of Mycobacterium marinum enzyme with that from Mycobacterium tuberculosis, pH 8.0, 28°C
0.0028
p-Aminobenzoic acid
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using control cholangiocarcinoma KKU-100 cells without cytokine administration, Vmax: 211.2 pmol/min/mg
0.0033
p-Aminobenzoic acid
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using control cholangiocarcinoma KKU-100 cells after treatment with the mixture of inflammatory cytokines for 48 h, Vmax: 135.7 pmol/min/mg. A significant decrease in Vmax is shown but without affecting Km, implying a change in the amount of enzyme but not in its affinity
0.283
p-Aminobenzoic acid
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strain 60.62, pH 7.4, 37°C
51.6
p-Aminobenzoic acid
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strain YG1024, pH 7.4, 37°C
additional information
additional information
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kinetic data of various acetyl donors with 4-nitroaniline or aniline as acceptor amine and of various acceptor amines with acetyl-CoA as donor
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additional information
additional information
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kinetic data of isozymes NAT1 and 2
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additional information
additional information
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not detectable: 4-aminosalicylic-acid, pH 7.4, 37°C
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additional information
additional information
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not detectable: 5-aminosalicylic acid
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additional information
additional information
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not detectable: 5-aminosalicylic acid
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additional information
additional information
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not detectable: 5-aminosalicylic acid
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additional information
additional information
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not detectable: p-aminobenzoic acid
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additional information
additional information
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kinetics analysis, kinetic mechanism, determination of the half-life of the acetylated enzyme intermediate as 270 s, overview
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additional information
additional information
steady-state kinetics, kinetic and isotope effect studies
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additional information
additional information
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NAT1 shows much higher affinity for p-amino benzoic acid than for sulfamethazine while NAT2 shows the opposite selectivity
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