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2.3.1.47: 8-amino-7-oxononanoate synthase

This is an abbreviated version!
For detailed information about 8-amino-7-oxononanoate synthase, go to the full flat file.

Word Map on EC 2.3.1.47

Reaction

pimeloyl-[acyl-carrier protein]
+
L-alanine
=
8-Amino-7-oxononanoate
 +
CO2
 +
holo-[acyl-carrier protein]

Synonyms

7,8-diaminopelargonic acid aminotransferase, 7-KAP synthetase, 7-keto-8-aminopelargonate synthase, 7-keto-8-aminopelargonate synthetase, 7-keto-8-aminopelargonic acid synthase, 7-keto-8-aminopelargonic acid synthetase, 7-keto-8-aminopelargonic synthetase, 7-oxo-8-aminononanoate synthase, 8-amino-7-oxononanoate synthase, 8-amino-7-oxopelargonate synthase, AON synthase, AONS, AOP synthase, AtbioF, BioF, bioF protein, DAPA AT, KAPA synthase, KAPA synthetase, KAPAS, More, SxtA, synthase, 7-oxo-8-aminononanoate, synthetase, 7-keto-8-aminopelargonate

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.47 8-amino-7-oxononanoate synthase

Crystallization

Crystallization on EC 2.3.1.47 - 8-amino-7-oxononanoate synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
enzyme in complex with inhibitor trifluoroalanine, soaking of holo-enzyme crystals in a solution containing 10 mM L-trifluoroalanine and 10 mM pyridoxal-5'-phosphate in crystallization buffer containing 1.6 M ammonium sulfate and 200 mM 1,3-bis[tris(hydroxymethyl)methylamino]propane at pH 8.5, and rapidly frozen in liquid nitrogen, X-ray diffraction structure determination and analysis at 1.99 A resolution
-
Mg2+ added for structure analysis of the holoenzyme
-
pH 8.5-8.9, ammonium sulfate precipitation, apoenzyme, X-ray diffraction structure analysis with and without bound pyridoxyl-5'-phosphate, model, active site residues
pyridoxal-5'-phosphate-bound form, pH 7.5, precipitation with polyethylene glycol, 0.2 M (NH4)2SO4 , x-ray diffraction structure analysis
-
purified recombinant enzyme, hanging-drop vapour diffusion, mixing of 0.002 ml of 5 mg/ml protein in 20 mM Tris-HCl pH 7.9, 100 mM NaCl, 2 mM DTT, with 0.002 ml of reservoir solution containing 150 mM DL-malic acid pH 7.4, 100 mM NaCl, 25% PEG 3350, equilibrqtion against 0.2 ml of reservoir solution, X-ray diffraction structure determination and analysis at 2.3 A resolution
the crystal structure of MsBioF is solved by molecular replacement using the structure of EcAONS, PDB code 1BS0, as the search model