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2.3.1.46: homoserine O-succinyltransferase

This is an abbreviated version!
For detailed information about homoserine O-succinyltransferase, go to the full flat file.

Word Map on EC 2.3.1.46

Reaction

succinyl-CoA
+
L-homoserine
=
CoA
+
O-succinyl-L-homoserine

Synonyms

homoserine O-succinyltransferase, homoserine O-transsuccinylase, homoserine succinyltransferase, homoserine transsuccinylase, homoserine-O-succinyltransferase, HST, HTS, MetA, MetA protein, succinyltransferase, homoserine

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.46 homoserine O-succinyltransferase

Purification

Purification on EC 2.3.1.46 - homoserine O-succinyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
Escherichia coli cells are lysed with 0.5 mg/ml lysozyme-1 mM phenylmethylsulfonyl fluoride-DNase I, sonicated, centrifuged, supernatants purified with Ni-nitrilo-triacetic acid agarose, gravity filtration of unbound protein, elution with 50 mM Tris-HCl, pH 7.5, NaCl, and imidazole, dialysis with potassium-phosphate buffer, pH 7.6
-
recombinant from E. coli BL21 (DE3)
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by 2 different steps of anion exchange chromatography
-
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by anion exchange chromatography, ammonium sulfate fractionation, hydrophobic interaction chromatography, and dialysis to over 90% purity
-
transformed Escherichia coli cells are lysed with 0.5 mg/ml lysozyme-1 mM phenylmethylsulfonyl fluoride-DNase I and sonicated, centrifuged, supernatants purified with Ni-nitrilo-triacetic acid agarose, gravity filtration of unbound protein, elution with 50 mM Tris-HCl, pH 7.5, NaCl, and imidazole, dialysis with potassium-phosphate buffer, pH 7.6
-