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2.3.1.43: phosphatidylcholine-sterol O-acyltransferase

This is an abbreviated version!
For detailed information about phosphatidylcholine-sterol O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.43

Reaction

phosphatidylcholine
+
a sterol
=
1-acylglycerophosphocholine
+
a sterol ester

Synonyms

acyltransferase, lecithin-cholesterol, cholesterol transacyltransferase, LAT, LCAT, lecithin cholesterol acyl transferase, lecithin cholesterol acyltransferase, lecithin-cholesterol acyl transferase, lecithin-cholesterol acyltransferase, lecithin/cholesterol acyltransferase, lecithin: cholesterol acyltransferase, lecithin:cholesterol acyl-transferase, lecithin:cholesterol acyltransferase, lysolecithin acyltransferase, phospholipid-cholesterol acyltransferase, plasma lecithin-cholesterol acyltransferase, TgLCAT, TGME49_272420

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.43 phosphatidylcholine-sterol O-acyltransferase

Purification

Purification on EC 2.3.1.43 - phosphatidylcholine-sterol O-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
affinity chromatography using a p-aminophenylarsenoxide-linked phase
-
affinity chromatography using an anti-apolipoprotein D immunoglobulin-Sepharose column
-
antibody affinity chromatography
-
extraction from cell membranes with Tween 20, purification by Sephadex G-100 and DEAE-Sepharose columns
-
further purification of a recombinant enzyme using ACA-44 allows to remove a proteoglycan contaminant
-
HDL-Sepharose, agglutinin-Sepharose chromatography and polyacrylamide electrophoresis
-
native enzyme from plasma
-
native enzyme from plasma to homogeneity
-
partial, using phenyl-Sepharose chromatography
phenyl-Sepharose and hydroxyapatite column chromatography
-
precipitation from plasma and purification by phenyl-agarose, DEAE-cellulose and hydroxyapatite columns
-
precipitation from plasma and purification by phenyl-sepharose and concanavalin A-Sepharose columns
-
precipitation from plasma with dextran sulfate-Ca2+ followed by DEAE-Sephadex A-50 and ion-exchange chromatography, rat enzyme is not absorbed on hydroxyapatite, which is employed for the purification of the human enzyme
-
precipitation from plasma with dextran sulfate-Mg2+ followed by several chromatographic steps, including DEAE-Sepharose, phenyl-Sepharose and affinity chromatography
protein A affinity column chromatography, HisTrap column chromatography and Superdex S200 gel filtration
purification of a recombinant C-terminal histidine tagged enzyme using a cobalt affinity resin
-
purification of recombinant enzymes
-
recombinant enzyme from HEK-293Fcells
recombinant enzyme from human lung cell line H1299 culture medium
recombinant enzyme from Spodoptera frugiperda Sf21 cells by immunoaffinity chromatography, recombinant His-tagged peptide TgLCAT(106-202) from Escherichia coli strain M15 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography
recombinant LCAT from the conditioned medium of engineered human lung cells
-
recombinant partially deglycosylated His-tagged enzyme from HEK-293 GnTI cells by nickel affinity chromatography to homogeneity
recombinant wild-type and mutant enzymes from COS-1 cells
-
Sephadex G-100, hydroxyapatite and affinity column chromatography
-
several chromatographic steps, including affinity chromatography
-