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2.3.1.42: glycerone-phosphate O-acyltransferase

This is an abbreviated version!
For detailed information about glycerone-phosphate O-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.42

Reaction

acyl-CoA
+
glycerone phosphate
=
CoA
+
acylglycerone phosphate

Synonyms

acyltransferase, dihydroxyacetone phosphate, DAP-AT, DAT, DHAP acyltransferase, DHAP-AT, DHAPAT, dihydroxyacetone phosphate acyl-transferase, dihydroxyacetone phosphate acyltransferase, dihydroxyacetone-phosphate acyltransferase, dihydroxyacetonephosphate acyltransferase, glyceronephosphate O-acyltransferase, GNPAT, LmDAT, male sterility protein, TtFARAT

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.42 glycerone-phosphate O-acyltransferase

Purification

Purification on EC 2.3.1.42 - glycerone-phosphate O-acyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme partially by subcellular fractionation
-
Ni-Sepharose resin column chromatography, His-Trap column chromatography and Superdex S200 gel filtration
partial, solubilized with sodium cholate-KCl
-
peroxisomal from placenta, with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, i.e. CHAPS, plus 1 M KCl
-
peroxisomal, partial
-
with 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate, i.e. CHAPS, plus 0.15 M NaCl
-
with taurocholate
-