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2.3.1.41: beta-ketoacyl-[acyl-carrier-protein] synthase I

This is an abbreviated version!
For detailed information about beta-ketoacyl-[acyl-carrier-protein] synthase I, go to the full flat file.

Word Map on EC 2.3.1.41

Reaction

an acyl-[acyl-carrier protein]
+
a malonyl-[acyl-carrier protein]
=
a 3-oxoacyl-[acyl-carrier protein]
+
CO2
+
an [acyl-carrier protein]

Synonyms

3-ketoacyl-ACP synthase, 3-ketoacyl-acyl carrier protein synthase, 3-ketoacyl-acyl-carrier protein synthase I, 3-oxoacyl synthase, 3-oxoacyl-[acyl-carrier-protein] synthase, acyl-malonyl(acyl-carrier-protein)-condensing enzyme, B-ketoacyl-ACP synthase I, beta-ketoacyl ACP synthase I, beta-ketoacyl acyl carrier protein synthase, beta-ketoacyl acyl carrier protein synthase I, beta-ketoacyl synthase, beta-ketoacyl synthetase, beta-ketoacyl [ACP] synthase, beta-ketoacyl-ACP synthase, beta-ketoacyl-ACP synthase I, beta-ketoacyl-ACP synthetase, beta-ketoacyl-ACP-synthase, beta-ketoacyl-ACP-synthase I, beta-ketoacyl-acyl carrier protein synthase I, beta-ketoacyl-acyl carrier protein synthetase, beta-ketoacyl-acyl-ACP synthase I, beta-ketoacyl-CoA synthase-I, beta-ketoacyl-[acyl carrier protein (ACP)] synthase III, beta-ketoacyl-[acyl carrier protein] synthase, beta-ketoacyl-[acyl carrier protein] synthase I, beta-ketoacyl-[acyl-carrier-protein] synthase I, beta-ketoacylsynthase, condensing enzyme, Cys-His-His-type beta-ketoacyl-acyl carrier protein synthase, DpsC, elongase, elongating beta-ketoacyl-acyl carrier protein synthase, FabB, FabBF, FabH, fatty acid condensing enzyme, KAS, Kas A, KAS I, KasA, KasI, KASI-1, KASI-2, KCSI, More, OsKASI, synthase, 3-oxoacyl-[acyl-carrier-protein]

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.41 beta-ketoacyl-[acyl-carrier-protein] synthase I

Cloned

Cloned on EC 2.3.1.41 - beta-ketoacyl-[acyl-carrier-protein] synthase I

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA cloned into lambda2AB-II
-
complements KAS II defect in Escherichia coli
construction of a cDNA library from developing seeds, DNA sequence analysis, expression as His-tagged enzyme in Escherichia coli
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expressed in an Arabidopsis thaliana kasI mutant
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expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli Rosetta BL21 (DE3)R3 cells
expressed in Nicotiana tabacum
expression as His-tagged protein in Escherichia coli
-
expression in Escherichia coli
-
expression of His-tagged FabH1 and FabH2 in Escherichia coli INValphaF'
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expression of His-tagged synthase IV in Escherichia coli BL21 (DE3)
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expression of synthase I and mutants as His-tagged proteins in Escherichia coli
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expression of synthase III-like polyketid synthase in Streptomyces lividans
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expression of the His-tagged enzyme in Escherichia coli strains XL-1 blue and M15
expression of wild-type and mutant synthase II in Escherichia coli
-
gene fabBF, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble NusA-fusion protein FabBF in Escherichia coli strain BL21(DE3)
-
gene KASI, DNA and amino acid sequence determination and analysis, sequence comparison, quantitative real-time PCR enzyme expression analysis, recombinant overexpression in transgenic Nicotiana tabacum plants under the control of the seed-specific napin promoter, leading to an increase in short-chain and medium-chain fatty acids from C6:0 to C16:0 compared with untransformed plants. CocoKASI transcript expression varies about 100fold between transgenic plants, with eight plants exhibiting detectable levels of CocoKASI expression
genes from hybrid of subssp. indica and japonica, and wild-type subsp. indica cv. Kasalath, DNA and amino acid sequenc determination and analysis, recombinant expression in rice plants using Agrobacterium tumefaciens transfection method
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His-tagged protein expressed in Escherichia coli BL21(DE3)
His-tagged protein expressed in Escherichia coli pLysS
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His-tagged proteinsexpressed in Mycobacterium smegmatis mc2155
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overexpression of fabH in Escherichia coli as His-tagged protein, synthase III
-
overexpression of His-tagged FabH in Escherichia coli BL21 (DE3)
plasmid-based expression of synthase III wild-type and mutants in Streptomyces glaucescens and His-tagged in Escherichia coli
-
recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
synthase III, overexpression in Escherichia coli strain JM101, construction of binary expression vectors for transformation of Brassica napus
-
synthase III, overexpression of His-tagged FabH in Escherichia coli BL21 (DE3)
synthases I, II, and III, recombinant expression
-