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2.3.1.4: glucosamine-phosphate N-acetyltransferase

This is an abbreviated version!
For detailed information about glucosamine-phosphate N-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.4

Reaction

acetyl-CoA
+
D-glucosamine 6-phosphate
=
CoA
+
N-acetyl-D-glucosamine 6-phosphate

Synonyms

acetyltransferase, glucosamine phosphate, AfGNA1, aminodeoxyglucosephosphate acetyltransferase, AtGNA1, CaGNA1, CDS L316, cgd4_4000, D-glucosamine-6-P N-acetyltransferase, GlcNAc-6-phosphate N-acetyltransferase, GlmU, glucosamine 6-phosphate acetylase, glucosamine 6-phosphate N-acetyltransferase, glucosamine phosphate acetyltransferase, glucosamine-6-phosphate acetylase, glucosamine-6-phosphate acetyltransferase, glucosamine-6-phosphate N-acetyltransferase, glucosamine-6-phosphate N-acetyltransferase 1, GNA, GNA1, HsGNA1, N-acetylglucosamine-6-phosphate synthase, OsGNA1, PF3D7_0629000, phosphoglucosamine acetylase, phosphoglucosamine N-acetylase, phosphoglucosamine transacetylase, ScGNA1, TGGT1_243600

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.4 glucosamine-phosphate N-acetyltransferase

Purification

Purification on EC 2.3.1.4 - glucosamine-phosphate N-acetyltransferase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acetone, DEAE-cellulose
-
ammonium sulfate, calcium phosphate gel, heat treatment at 65°C
-
ammonium sulfate, Ultragel AcA54, CoA-Sepharose
-
calcium phosphate gel, heat treatment, 60°C, mixed bed resin, alumina Cgamma gel, ammonium sulfate
-
hydroxylapatite, ammonium sulfate, cellulose phosphate chromatography, electrofocusing, gel electrophoresis
-
isoenzymes 1-3, CM-Sephadex
-
protamine sulfate, 60°C, pH 4.8, mixed-bed resin, ammonium sulfate
-
protamine sulfate, pH 4.9, ammonium sulfate, acetone, CM-Sphadex, partially purified
-
recombinant His-tagged enzyme
using a HIS-Select HF affinity gel column
-
using affinity, ion-exchange and size-exclusion chromatography: yield 25 mg of pure HsGNA1 per litre of bacterial culture
using affinity, ion-exchange and size-exclusion chromatography: yield 4 mg of pure AfGNA1 per litre of bacterial culture
-