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0.0055 - 0.0742
2-hydroxybutanoyl-CoA
0.00054 - 0.0093
butanoyl-CoA
0.0075 - 0.0301
glutaryl-CoA
0.0018 - 0.0172
octanoyl-CoA
0.00077 - 0.015
pyridoxal 5'-phosphate
0.00064 - 52.4
succinyl-CoA
additional information
additional information
-
0.0055
2-hydroxybutanoyl-CoA
-
mutant R85K, pH 7.5, 30°C
0.0061
2-hydroxybutanoyl-CoA
-
mutant R85L, pH 7.5, 30°C
0.0098
2-hydroxybutanoyl-CoA
-
wild-type, pH 7.5, 30°C
0.0742
2-hydroxybutanoyl-CoA
-
mutant R85L/T430V, pH 7.5, 30°C
0.00054
butanoyl-CoA
-
mutant R85L/T430V, pH 7.5, 30°C
0.0027
butanoyl-CoA
-
mutant R85K, pH 7.5, 30°C
0.0061
butanoyl-CoA
-
wild-type, pH 7.5, 30°C
0.0093
butanoyl-CoA
-
mutant R85L, pH 7.5, 30°C
0.0075
glutaryl-CoA
-
mutant R85K, pH 7.5, 30°C
0.017
glutaryl-CoA
-
wild-type, pH 7.5, 30°C
0.0301
glutaryl-CoA
-
mutant R85L, pH 7.5, 30°C
0.00032
glycine
-
pH 7.5, 30°C, ALAS(K313A mutant)/ALAS
0.00045
glycine
-
pH 7.5, 30°C, ALAS/ALAS
0.001
glycine
-
mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C
0.0015
glycine
-
mutant N427H, pH 7.5, 20°C
0.0017
glycine
-
mutant L437Q, pH 7.5, 20°C
0.0018
glycine
-
pH 7.5, 30°C, ALAS/ALAS (K313A mutant)
0.002
glycine
-
mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C
0.002
glycine
-
pH 7.5, 30°C, ALAS
0.0023
glycine
-
mutant A425T, pH 7.5, 20°C
0.0023
glycine
-
mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C
0.0026
glycine
-
mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C
0.0029
glycine
-
mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C
0.003
glycine
-
mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C
0.011
glycine
-
wild-type, pH 7.5, 20°C
0.0171
glycine
-
wild-type, cosubstrate octanoyl-CoA, pH 7.5, 30°C
0.0172
glycine
-
mutant R85K, cosubstrate butanoyl-CoA, pH 7.5, 30°C
0.0182
glycine
-
mutant R85K, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C
0.02
glycine
-
mutant R85K, cosubstrate succinyl-CoA, pH 7.5, 30°C
0.0212
glycine
-
mutant R85K, cosubstrate glutaryl-CoA, pH 7.5, 30°C
0.0221
glycine
-
wild-type, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C
0.0242
glycine
-
wild-type, cosubstrate succinyl-CoA, pH 7.5, 30°C
0.0252
glycine
-
mutant R85K, cosubstrate octanoyl-CoA, pH 7.5, 30°C
0.0284
glycine
-
wild-type, cosubstrate glutaryl-CoA, pH 7.5, 30°C
0.0293
glycine
-
wild-type, cosubstrate butanoyl-CoA, pH 7.5, 30°C
0.0552
glycine
-
mutant R85L, cosubstrate octanoyl-CoA, pH 7.5, 30°C
0.0591
glycine
-
mutant R85L, cosubstrate 2-hydroxybutanoyl-CoA, pH 7.5, 30°C
0.0631
glycine
-
mutant R85L, cosubstrate succinyl-CoA, pH 7.5, 30°C
0.0703
glycine
-
mutant R85L, cosubstrate butanoyl-CoA, pH 7.5, 30°C
0.0706
glycine
-
mutant R85L, cosubstrate glutaryl-CoA, pH 7.5, 30°C
0.0742
glycine
-
mutant cosubstrate octanoyl-CoA, R85L/T430V, pH 7.5, 30°C
0.0882
glycine
-
mutant cosubstrate butanoyl-CoA, R85L/T430V, pH 7.5, 30°C
0.0922
glycine
-
mutant cosubstrate 2-hydroxybutanoyl-CoA, R85L/T430V, pH 7.5, 30°C
0.0984
glycine
-
mutant cosubstrate succinyl-CoA, R85L/T430V, pH 7.5, 30°C
2 - 3
glycine
-
pH 7.5, 30°C, wild-type
2 - 3
glycine
-
pH 7.5, 30°C, ALAS
2 - 3
glycine
wild-type enzyme, pH 7.5, 18°C
2.31
glycine
-
wild type enzyme, at pH 7.5 and 37°C
2.5
glycine
-
papain untreated enzyme
2.63
glycine
-
mutant enzyme H29R, at pH 7.5 and 37°C
3.25
glycine
-
mutant enzyme H15K, at pH 7.5 and 37°C
3.31
glycine
wild type enzyme, at pH 7.5 and 25°C
3.4
glycine
mutant enzyme C398A, at pH 7.5 and 25°C
3.52
glycine
mutant enzyme H340A, at pH 7.5 and 25°C
3.57
glycine
mutant enzyme H340A/C398A, at pH 7.5 and 25°C
6.2
glycine
-
recombinant mutant G144T
6.5
glycine
-
erythroid-specific isoform
6.95
glycine
-
recombinant mutant dimer K149A/K313A
7.5
glycine
-
papain treated enzyme
7.5
glycine
pH 7.4, 37°C, recombinant mutant DELTAAGTG
7.7
glycine
pH 7.4, 37°C, recombinant mutant F557X
8
glycine
pH 7.5, 37°C, wild-type enzyme
8
glycine
wild type enzyme, at pH 7.5 and 37°C
9.3
glycine
-
recombinant mutant G144A
9.3
glycine
pH 7.4, 37°C, recombinant wild-type enzyme
11
glycine
mutant N150G, pH 7.5, 18°C
11
glycine
mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C
11.3
glycine
-
mutant enzyme T363S, at pH 7.2 and 37°C
11.7
glycine
-
recombinant erythroid isoform
11.7
glycine
-
pH 7.5, 20°C, mutant 2XALAS
11.8
glycine
-
pH 7.5, 30°C, ALAS/ALAS (K313A mutant)
11.9
glycine
-
recombinant mutant G144S
12
glycine
-
mutant V423L/Y428R/P432E/R433I/G434N/E435Q/L437K, pH 7.5, 20°C
12
glycine
mutant N150F, pH 7.5, 18°C
12
glycine
pH 7.4, 37°C, recombinant mutant Q548X
12.5
glycine
-
recombinant erythroid isoform
13
glycine
-
mutant A425G/Y428H/R433H/G434N/E435K/L437K, pH 7.5, 20°C
13
glycine
-
mutant Y428N/P432N/R433I/G434E/E435K/L437K, pH 7.5, 20°C
13
glycine
pH 7.4, 37°C, recombinant mutant DELTAAT
13.5
glycine
pH 7.4, 37°C, recombinant mutant DELTAG
14
glycine
-
pH 7.5, 20°C, wild-type
14
glycine
-
mutant V423I/A425P/Y428C/P432R/R433K/E435N, pH 7.5, 20°C
14
glycine
-
wild-type, pH 7.5, 20°C
14
glycine
-
mutant enzyme T83S, at pH 7.2 and 37°C
14.4
glycine
-
recombinant erythroid isoform mutant R433K
14.8
glycine
-
pH 7.5, 30°C, ALAS(K313A mutant)/ALAS
15
glycine
-
mutant N427H, pH 7.5, 20°C
16
glycine
-
mutant R433K/G434K/E435Q/L437Q, pH 7.5, 20°C
16
glycine
-
mutant Y428I/R433Q /G434N/E435T/L437N, pH 7.5, 20°C
16
glycine
mutant N150A, pH 7.5, 18°C
16
glycine
mutant N150H, pH 7.5, 18°C
16.7
glycine
-
pH 7.5, 30°C, mutant 2XALAS
16.7
glycine
-
pH 7.5, 30°C, ALAS/ALAS
16.7
glycine
-
mutant enzyme T83S/T363S, at pH 7.2 and 37°C
18
glycine
-
mutant S254A, 30°C, pH 7.5
18.4
glycine
-
recombinant erythroid isoform mutant R433L
23
glycine
-
recombinant erythroid isoform wild-type
24
glycine
-
mutant L437Q, pH 7.5, 20°C
25
glycine
-
wild-type, 30°C, pH 7.5
25
glycine
-
mutant A425T, pH 7.5, 20°C
26.4
glycine
isoform HemA, pH and temperature not specified in the publication
27
glycine
-
mutant S254T, 30°C, pH 7.5
31.7
glycine
-
wild type enzyme, at pH 7.2 and 37°C
31.7
glycine
isoform HemA, pH and temperature not specified in the publication
39
glycine
mutant N150W, pH 7.5, 18°C
43.4
glycine
isoform HemA, pH and temperature not specified in the publication
46.8
glycine
mutant C281P of isoform HemT, pH and temperature not specified in the publication
50
glycine
-
recombinant enzyme
50
glycine
pH 7.5, 37°C, mutant K221V
51
glycine
-
recombinant protein
52.2
glycine
-
recombinant mutant Y121H
52.2
glycine
-
recombinant mutant G142C
52.5
glycine
isoform HemT, pH and temperature not specified in the publication
103
glycine
-
recombinant erythroid isoform mutant R439K
140
glycine
-
recombinant erythroid isoform mutant D279E
400
glycine
-
recombinant mutant Y121F
0.0018
octanoyl-CoA
-
mutant R85L, pH 7.5, 30°C
0.0034
octanoyl-CoA
-
wild-type, pH 7.5, 30°C
0.0103
octanoyl-CoA
-
mutant R85K, pH 7.5, 30°C
0.0172
octanoyl-CoA
-
mutant R85L/T430V, pH 7.5, 30°C
0.00077
pyridoxal 5'-phosphate
-
-
0.001 - 0.01
pyridoxal 5'-phosphate
-
-
0.003
pyridoxal 5'-phosphate
-
-
0.015
pyridoxal 5'-phosphate
-
-
0.00064
succinyl-CoA
-
recombinant mutant G144A
0.001
succinyl-CoA
pH 7.5, 37°C, wild-type enzyme
0.0011
succinyl-CoA
mutant N150W, pH 7.5, 18°C
0.0012
succinyl-CoA
-
recombinant erythroid isoform and mutant G144S
0.0012
succinyl-CoA
-
mutant S254T, 30°C, pH 7.5
0.0012
succinyl-CoA
mutant N150H, pH 7.5, 18°C
0.0013
succinyl-CoA
-
wild-type, 30°C, pH 7.5
0.0016
succinyl-CoA
-
recombinant mutant Y121H
0.0019
succinyl-CoA
-
recombinant mutant G144T
0.002
succinyl-CoA
-
recombinant erythroid isoform wild-type
0.002
succinyl-CoA
-
recombinant mutant G142C
0.002
succinyl-CoA
-
recombinant mutant G142C
0.002
succinyl-CoA
-
erythroid-specific isoform
0.0021
succinyl-CoA
mutant N150G, pH 7.5, 18°C
0.0022
succinyl-CoA
-
recombinant erythroid isoform mutant R433K
0.0022
succinyl-CoA
mutant N150F, pH 7.5, 18°C
0.0023
succinyl-CoA
wild-type enzyme, pH 7.5, 18°C
0.0024
succinyl-CoA
mutant enzyme H340A, at pH 7.5 and 25°C
0.00242
succinyl-CoA
wild type enzyme, at pH 7.5 and 25°C
0.00255
succinyl-CoA
mutant enzyme C398A, at pH 7.5 and 25°C
0.00258
succinyl-CoA
mutant enzyme H340A/C398A, at pH 7.5 and 25°C
0.0029
succinyl-CoA
-
wild-type, pH 7.5, 30°C
0.0032
succinyl-CoA
-
recombinant erythroid isoform mutant R433L
0.0038
succinyl-CoA
mutant N150A, pH 7.5, 18°C
0.0124
succinyl-CoA
-
mutant R85K, pH 7.5, 30°C
0.014
succinyl-CoA
-
recombinant mutant Y121F
0.0146
succinyl-CoA
-
wild type enzyme, at pH 7.2 and 37°C
0.0146
succinyl-CoA
isoform HemA, pH and temperature not specified in the publication
0.015
succinyl-CoA
isoform HemT, pH and temperature not specified in the publication
0.01513
succinyl-CoA
-
mutant enzyme H15K, at pH 7.5 and 37°C
0.0176
succinyl-CoA
isoform HemA, pH and temperature not specified in the publication
0.0184
succinyl-CoA
isoform HemA, pH and temperature not specified in the publication
0.0203
succinyl-CoA
-
mutant R85L, pH 7.5, 30°C
0.0214
succinyl-CoA
-
wild type enzyme, at pH 7.5 and 37°C
0.02227
succinyl-CoA
-
mutant enzyme H29R, at pH 7.5 and 37°C
0.023
succinyl-CoA
pH 7.5, 37°C, mutant K221V
0.0261
succinyl-CoA
mutant C281P of isoform HemT, pH and temperature not specified in the publication
0.027
succinyl-CoA
-
recombinant erythroid isoform mutant R439K
0.032
succinyl-CoA
-
mutant S254A, 30°C, pH 7.5
0.035
succinyl-CoA
-
recombinant erythroid isoform mutant D279E
0.0357
succinyl-CoA
pH 7.4, 37°C, recombinant mutant DELTAAGTG
0.0398
succinyl-CoA
pH 7.4, 37°C, recombinant mutant DELTAAT
0.0401
succinyl-CoA
pH 7.4, 37°C, recombinant mutant DELTAG
0.0407
succinyl-CoA
pH 7.4, 37°C, recombinant wild-type enzyme
0.055
succinyl-CoA
-
recombinant protein
0.0774
succinyl-CoA
-
mutant enzyme T83S, at pH 7.2 and 37°C
0.096
succinyl-CoA
-
mutant R85L/T430V, pH 7.5, 30°C
0.1
succinyl-CoA
-
recombinant enzyme
0.1302
succinyl-CoA
-
mutant enzyme T83S/T363S, at pH 7.2 and 37°C
0.1645
succinyl-CoA
-
mutant enzyme T363S, at pH 7.2 and 37°C
0.45
succinyl-CoA
-
pH 7.5, 30°C, mutant 2XALAS
0.63
succinyl-CoA
-
pH 7.5, 20°C, mutant 2XALAS
1
succinyl-CoA
wild type enzyme, at pH 7.5 and 37°C
1.3
succinyl-CoA
mutant enzyme DELTAmALAS2, at pH 7.5 and 37°C
1.52
succinyl-CoA
-
recombinant mutant dimer K149A/K313A
2.3
succinyl-CoA
-
pH 7.5, 30°C, wild-type
11
succinyl-CoA
-
pH 7.5, 20°C, wild-type
36.3
succinyl-CoA
pH 7.4, 37°C, recombinant mutant F557X
49.55
succinyl-CoA
pH 7.5
52.4
succinyl-CoA
pH 7.4, 37°C, recombinant mutant Q548X
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
-
-
additional information
additional information
-
overview
-
additional information
additional information
-
overview
-
additional information
additional information
-
overview
-
additional information
additional information
-
overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics, overview
-
additional information
additional information
kinetics analysis of wild-type and mutant enzymes, single and multiple turnover and stopped flow measurements, substrate protection study, overview
-
additional information
additional information
-
kinetics analysis of wild-type and mutant enzymes, single and multiple turnover and stopped flow measurements, substrate protection study, overview
-
additional information
additional information
Michaelis-Menten steady-state kinetics, two-step sequential kinetic mechanism , and thermodynamics. Both the reaction for wild-type hALAS2 and those for the X-linked protoporphyria variants comprised a single kinetic step associated with quinonoid intermediate formation followed by one decay step. Rates for the two steps range from 9.8-14.3/s and from 1.37-1.97 s for the reactions of the XLPP variants, whereas those for the wild-type hALAS2-catalyzed reaction are 6.6/s and 0.70/s
-
additional information
additional information
-
Michaelis-Menten steady-state kinetics, two-step sequential kinetic mechanism , and thermodynamics. Both the reaction for wild-type hALAS2 and those for the X-linked protoporphyria variants comprised a single kinetic step associated with quinonoid intermediate formation followed by one decay step. Rates for the two steps range from 9.8-14.3/s and from 1.37-1.97 s for the reactions of the XLPP variants, whereas those for the wild-type hALAS2-catalyzed reaction are 6.6/s and 0.70/s
-
additional information
additional information
pre-steady state and steady state kinetics, overview
-
additional information
additional information
-
pre-steady state and steady state kinetics, overview
-
additional information
additional information
pre-steady-state and steady-state kinetics of wild-type and mutant enzymes, stopped-flow measurements, single and mutiple turnover rates, equilibrium dissociation constants, binding isotherms, detailed overview
-
additional information
additional information
-
pre-steady-state and steady-state kinetics of wild-type and mutant enzymes, stopped-flow measurements, single and mutiple turnover rates, equilibrium dissociation constants, binding isotherms, detailed overview
-