2.3.1.31: homoserine O-acetyltransferase

This is an abbreviated version!
For detailed information about homoserine O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.31

2.3.1.31

l-methionine

ping-pong

o-acetylhomoserine

acetyl-enzyme

succinyl-coa

sulfhydrylase

gamma-hydroxyl

o-acetyl-l-homoserine

medicine

molecular biology

drug development

Reaction

Synonyms

acetyltransferase, homoserine, CnHTA, DcsE, HAT, homoserine acetyltransferase, homoserine O-acetyltransferase, homoserine transacetylase, homoserine transsuccinylase, homoserine-O-transacetylase, HTA, HTS, L-homoserine O-acetyltransferase, MaHTA, MaMetX, MET2, MetA, MetX, MetXA, metX_1, metX_2, MhHTA, MhMetX, MsHAT, MtHTA, MtMetX, TmHTS

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.31 homoserine O-acetyltransferase

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Results	in table
20179	AA Sequence
3	Application
1	CAS Registry Number
18	Cloned(Commentary)
5	Cofactor
10	Crystallization (Commentary)
64	General Information
1	General Stability
2	IC50 Value
30	Inhibitors
26	kcat/KM [mM/s]
6	Ki Value [mM]
98	KM Value [mM]
7	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
41	Organism
5	Pathway
32	PDB ID
12	pH Optimum
2	pH Range
1	pH Stability
75	Protein Variants
15	Purification (Commentary)
7	Reaction
1	Reaction Type
41	Reference
6	Specific Activity [micromol/min/mg]
1	Storage Stability
75	Substrates and Products (Substrate)
8	Subunits
95	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
3	Temperature Stability [°C]
60	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.31 - homoserine O-acetyltransferase

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3 entries

cells are centrifuged in 50 mM Tris, pH 8.0, sonicated, centrifuged, supernatant loaded to a Ni2+-nitrilotriacetic acid-agarose column, washed, and eluted, eland incubated with protease and dialyzed, reloaded onto a Ni2+-nitrilotriacetic acid-agarose column, dialyzed, loaded onto Superdex-200 size-exclusion fast protein liquid chromatography column with 25 mM HEPES-buffer, pH 7.5

Bacillus cereus

687796

partial

3 entries

partial, mutant strain 6

Saccharomyces cerevisiae

486784

recombinant from E. coli

Haemophilus influenzae

486788

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity and anion excange chromatography, followed by gel electrophoresis, dialysis, and ultrafiltration

Staphylococcus aureus

735393

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3)-T1 by nickel affinity chromatography and two different steps of gel filtration

Mycolicibacterium smegmatis

A0A8B4QGM8

755982

recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography, dialysis and ultrafiltration

Streptomyces lavendulae

D2Z028

736358

using Ni-NTA chromatography

Cryptococcus neoformans

684480

using Ni-NTA-chromatography

Bacillus cereus

687796