2.3.1.31: homoserine O-acetyltransferase

This is an abbreviated version!
For detailed information about homoserine O-acetyltransferase, go to the full flat file.

Reaction

Synonyms

acetyltransferase, homoserine, CnHTA, DcsE, HAT, homoserine acetyltransferase, homoserine O-acetyltransferase, homoserine transacetylase, homoserine transsuccinylase, homoserine-O-transacetylase, HTA, HTS, L-homoserine O-acetyltransferase, MaHTA, MaMetX, MET2, MetA, MetX, MetXA, metX_1, metX_2, MhHTA, MhMetX, MsHAT, MtHTA, MtMetX, TmHTS

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.31 homoserine O-acetyltransferase

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Results	in table
20179	AA Sequence
3	Application
1	CAS Registry Number
18	Cloned(Commentary)
5	Cofactor
10	Crystallization (Commentary)
64	General Information
1	General Stability
2	IC50 Value
30	Inhibitors
26	kcat/KM [mM/s]
6	Ki Value [mM]
98	KM Value [mM]
7	Molecular Weight [Da]
28	Natural Substrates/ Products (Substrates)
41	Organism
5	Pathway
32	PDB ID
12	pH Optimum
2	pH Range
1	pH Stability
75	Protein Variants
15	Purification (Commentary)
7	Reaction
1	Reaction Type
41	Reference
6	Specific Activity [micromol/min/mg]
1	Storage Stability
75	Substrates and Products (Substrate)
8	Subunits
95	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
3	Temperature Stability [°C]
60	Turnover Number [1/s]

KCat KM Value

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KCat KM Value on EC 2.3.1.31 - homoserine O-acetyltransferase

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kcat/KM VALUE [1/mMs^-1]

SUBSTRATE

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

IMAGE

0.0228 - 5.28

acetyl-CoA

10 entries

0.0074 - 4.56

L-homoserine

15 entries

2.94

succinyl-CoA

Bacillus cereus

-

E111G, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.0228

acetyl-CoA

Bacillus cereus

-

K47M, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.139

acetyl-CoA

Bacillus cereus

-

E237A, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.361

acetyl-CoA

Bacillus cereus

-

E237Q, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.485

acetyl-CoA

Bacillus cereus

-

K47R, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.511

acetyl-CoA

Bacillus cereus

-

R249M , 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.91

acetyl-CoA

Bacillus cereus

-

E237D, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

1.23

acetyl-CoA

Bacillus cereus

-

K163M, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

2.18

acetyl-CoA

Bacillus cereus

-

S192A, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

2.64

acetyl-CoA

Bacillus cereus

-

E250A, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

5.28

acetyl-CoA

Bacillus cereus

-

wild-type, 50 mM potassium phosphate buffer, pH 7.5, 2 mM L-homoserine, 25°C

687796

0.0074

L-homoserine

Bacillus cereus

-

R249M, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.009

L-homoserine

Streptomyces lavendulae

D2Z028

recombinant His-tagged mutant G52A enzyme, pH 8.0, 30°C

736358

0.015

L-homoserine

Streptomyces lavendulae

D2Z028

recombinant His-tagged wild-type enzyme, pH 8.0, 30°C

736358

0.0176

L-homoserine

Bacillus cereus

-

E237A, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.0507

L-homoserine

Bacillus cereus

-

K47M, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.118

L-homoserine

Bacillus cereus

-

K163M, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.147

L-homoserine

Bacillus cereus

-

E250A, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.18

L-homoserine

Bacillus cereus

-

E237Q, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.391

L-homoserine

Bacillus cereus

-

E237D, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.4

L-homoserine

Streptomyces lavendulae

D2Z028

recombinant His-tagged mutant G52A/P55G enzyme, pH 8.0, 30°C

736358

0.533

L-homoserine

Bacillus cereus

-

S192A, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

0.63

L-homoserine

Streptomyces lavendulae

D2Z028

recombinant His-tagged mutant P55G enzyme, pH 8.0, 30°C

736358

0.692

L-homoserine

Bacillus cereus

-

K47R, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796

4.02

L-homoserine

Bacillus cereus

-

E111G, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM succinyl-CoA, 25°C

687796

4.56

L-homoserine

Bacillus cereus

-

wild-type, 50 mM potassium phosphate buffer, pH 7.5, 0.5 mM acetyl-CoA, 25°C

687796