Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

2.3.1.30: serine O-acetyltransferase

This is an abbreviated version!
For detailed information about serine O-acetyltransferase, go to the full flat file.

Word Map on EC 2.3.1.30

Reaction

acetyl-CoA
+
L-serine
=
CoA
 +
O-acetyl-L-serine

Synonyms

acetyltransferase, serine, AtOASS, AtSAT, AtSAT1, AtSerat 2;1, BvSAT, CysE, cytosolic serine acetyltransferase, DcsE, EhSAT1, EhSAT2, EhSAT3, GmSerat2,1, HISAT, Kpn CysE, L-serine acetyltransferase, L-serine O-acetyltransferase, L-serineO-acetyltransferase, More, plastidic serine acetyltransferase, SAT, SAT isoform 1, SAT-1, SAT1, SAT2, SAT3, SATase, SERAT, Serat1,1, SERAT1.1, Serat2,1, Serat2,2, SERAT2.1, Serat3,1, Serat3,2, serine acetlytransferase, serine acetyltransferase, serine acetyltransferase 1, serine acetyltransferase 2,1, serine O-acetyltransferase, serine O-acetyltransferase 1, serine transacetylase, Spn cysE, SP_0589

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.30 serine O-acetyltransferase

Cloned

Cloned on EC 2.3.1.30 - serine O-acetyltransferase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
C-terminal deletion mutants, expression in Escherichia coli strain JM70
-
cloning and DNA sequencing of 3 isozymes, complementation of Cys- mutant Escherichia coli strain JM39 with the 3 isozymes, sequence analysis of cytosolic isozyme
-
construction of an inactivated mutant by targeted disruption of CysE gene for usage in complementation assays
-
construction of cysE gene mutants by random PCR mutagenesis, expression in Escherichia coli cysteine auxotrophic strain JM39-8
-
cotyledon segments of seedlings of Ipomaea aquatica are transformed with Arabidopsis serine acetyltransferase and rice cysteine synthase genes under the control of the cauliflower mosaic virus 35S promoter. Simultaneous expression of serine acetyltransferase and cysteine synthase results in enhanced sulfate uptake and increased biomass in Ipomaea aquatica
-
expressed as a His-tagged fusion protein
-
expression as glutathione-S-transferase fusion protein, amino acid sequence
-
expression in Cys- Escherichia coli mutant and functional complementation, transient expression as GFP-fusion and beta-glucuronidase-fusion protein in plants
-
expression in Escherichia coli
expression in Escherichia coli as a fusion protein with glutathione-S-transferase
-
expression in Escherichia coli SAT inactivated mutant
expression in Escherichia coli, functional complementation of Escherichia coli cysA- mutant, DNA and amino acid sequence detemination
O13389
expression in inactivated Escherichia coli mutant, insensitive against inhibition by L-cysteine
expression in inactivated Escherichia coli mutant, unaltered L-cysteine sensitivity compared to wild-type
expression in Saccharomyces cerevisiae.Increase in cysteine production confers enhanced resistance against osmotic stress in the osmosensitive yeast strain
-
expression in Salmonella typhimurium Cys- strain DW 18.1 from plasmid
-
expression of 3 isozymes SAT-m, SAT-p, SAT-c as GFP-fusion proteins in Arabidopsis thaliana
-
expression of His-tagged protein encoded on gene cmSAT in Escherichia coli, functional complementation of SAT-deficient Escherichia coli strain JM15, DNA and amino acid sequence determination
-
expression of mutant enzymes in Escherichia coli
expression of SAT1 gene as glutathione-S-transferase fusion protein in Escherichia coli DH5alpha, complementation of serine acetyltransferase mutant Escherichia coli strain JM39, single copy gene, amino acid sequence determination
expression of wild-type and mutants in Escherichia coli
expression of wild-type and mutants in Escherichia coli SAT inactivated mutant as S-tagged protein
gene cysE or MSMEG_5947, DNA and amino acid sequence determination and analysis, functional recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene cysE or Rv2335, DNA and amino acid sequence determination and analysis, recombinant expression of His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene cysE or SP_0589, recombinant expression of His-tagged enzyme in Escherichia coli strain codon optimized strain BL21(DE3)-RIL
gene cysE, recombinant expression of C-terminally His-tagged in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain NovaBlue using vector pET29b
-
gene cysE, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)
gene cysE, recombinant overexpression of Arabidopsis thaliana AtSAT1 in Zea mays leaves lines OE1 and OE3 under control of the leaf bundle sheath cell-specific rbcS1 promoter resulting in 12fold higher SAT activity, increased S-assimilation in the leaves, and higher levels of storage protein mRNA and storage proteins, particularly the 10-kDa D-zein, during endosperm development. Quantitative RT-PCR expression analysis, the measured enzyme activity is a combination of endogenous SAT and that derived from expression of AtSAT1
gene CysE, sequence comparisons, recombinant expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3)
gene dcsE, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysS
gene FgSAT or FGSG_00186, DNA and amino acid sequence determination and analysisphylogenetic analysis and tree, real-time PCR expression analysis, genetic complementation of FgSAT deletion mutant
gene LSEI 0479 lying upstream of cysK is predicted to encode a homoserine trans-succinylase (metA). The gene is cloned from Lactobacillus casei strain FAM18110, and recombinantly expressed in Escherichia coli strain BL21(DE3). The purified, recombinant protein does not acylate L-homoserine in vitro. Instead, it catalyzes the formation of O-acetyl serine from L-serine and acetyl-CoA. Furthermore, the plasmid expressing the Lactobacillus casei gene complements an Escherichia coli cysE mutant strain but not an Escherichia coli metA mutant. This clearly demonstrates that the gene annotated as metA in fact encodes the SAT function and should be annotated as cysE
gene SAT1, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
insertional inactivation of wild-type CysE gene from Escherichia coli C600
-
isolation of cDNA clone from Citrullus vulgaris genetic library by complementation of a Cys- Escherichia coli strain JM 39/5, single copy gene, DNA and amino acid sequence determination
-
isozyme VvSERAT1-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme
isozyme VvSERAT2-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme
isozyme VvSERAT2-2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme
isozyme VvSERAT3-1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of GFP-tagged VvSERAT isozyme in Arabidopsis thaliana and in Vitis vinifera protoplasts with specific subcellular localization of the isozyme
nickel and cobalt resistance engineered in Escherichia coli by overexpression of serine acetyltransferase from the nickel hyperaccumulator plant Thlaspi goesingense
overexpression
-
overexpression in Escherichia coli
overexpression in Escherichia coli, construction of an expression vector which increases the expression to 17% of the soluble cell protein
-
overexpression of 3 isozymes SAT-m, SAT-p, SAT-c in Escherichia coli BL21 (DE3)
-
PCR-amplification, expression of histidine tagged gene in Escherichia coli BL21 (DE3) pLysS, heat shock transformed
PCR-amplification, plasmid transferred to Agrobacterium tumefaciens Ag10 for transfection of Lupinus angustifolius (cultivar Kalya)
recombinant expression
SAT1 is closely linked to SAT5, high DNA sequence homology