2.3.1.250: [Wnt protein] O-palmitoleoyl transferase
This is an abbreviated version!
For detailed information about [Wnt protein] O-palmitoleoyl transferase, go to the full flat file.
Word Map on EC 2.3.1.250
Reaction
Synonyms
CG6205, MG61, Mom-1, Mporc, mPorcD, PORC, PORCN, porcupine, PPN, protein-serine O-palmitoleoyltransferase porcupine, Xporc
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2.3.1.250 [Wnt protein] O-palmitoleoyl transferaseAdvanced search results
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results (Substrates Products
Substrates Products on EC 2.3.1.250 - [Wnt protein] O-palmitoleoyl transferase
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
(9Z)-hexadec-9-enoyl-CoA + [Wnt3]-L-serine
CoA + [Wnt3]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5]-L-serine
CoA + [Wnt5]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
decanoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-decanoyl-L-serine
about 30% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate
-
-
?
dodecanoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-dodecanoyl-L-serine
about 15% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate
-
-
?
hexanoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-hexanoyl-L-serine
about 20% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate
-
-
?
octanoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-octanoyl-L-serine
about 25% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate
-
-
?
tetradecanoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-tetradecanoyl-L-serine
about 10% of the activity as compared to (9Z)-hexadec-9-enoyl-CoA. There is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate
-
-
?
[125I]iodo-pentadecenoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-[125I]iodo-pentadecenoyl-L-serine
[125I]iodo-pentadecenoyl-CoA is a radioiodinated palmitoleate analog
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine
CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wg]-L-serine
CoA + [Wg]-O-(9Z)-hexadec-9-enoyl-L-serine
Wnt substrate from Drosophila melanogaster
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-)
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
chick WNT1 protein, the enzyme is an O-acyl transferase for WNT1 protein, WNT1 residues 214-234 are sufficient for PORCN-dependent palmitoylation of Ser224. Substitution of Ser224 with Thr, but not Cys, is tolerated in palmitoylation and biological assays. Transiently transfection of HEK-293T cells with WNT1. WNT1 mutant S224T is accepted as substrate, while mutants S224A and S224C are inactive
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-)
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt1]-L-serine
CoA + [Wnt1]-O-(9Z)-hexadec-9-enoyl-L-serine
chicken substrate, and chimeric recombinant substrate containing amino acids 1-63 from mouse Wnt1 (-EPSLQL) and amino acids 64-370 from chicken Wnt1 (LSRKQ-)
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
chicken substrate, and substrate from Mus musculus
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
Wnt3a is palmitoylated in the endoplasmic reticulum and targeted to exosomes, palmitoylated Wnt3a proteins are found throughout the cytosol and at the plasma membrane but not in the nucleus, overview
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
chicken substrate, and substrate from Mus musculus
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
Wnt3a is palmitoylated by fatty acids 13-16 carbons in length at Ser209 but not at Cys77, consistent with a slow turnover rate, glycosylation is not required for Wnt3a palmitoylation, which is necessary but not sufficient for Wnt3a secretion
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
the enzyme catalyzes the palmitoylation of Ser209 and Cys77 of Wnt3a expressed in L cells
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
the enzyme catalyzes the palmitoylation of the serine corresponding to Ser209 of Wnt3a
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
Wnt3a is palmitoylated in the endoplasmic reticulum and targeted to exosomes, palmitoylated Wnt3a proteins are found throughout the cytosol and at the plasma membrane but not in the nucleus, overview
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
chicken substrate, and substrate from Mus musculus
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt3a]-L-serine
CoA + [Wnt3a]-O-(9Z)-hexadec-9-enoyl-L-serine
Wnt3a is palmitoylated by fatty acids 13-16 carbons in length at Ser209 but not at Cys77, consistent with a slow turnover rate, glycosylation is not required for Wnt3a palmitoylation, which is necessary but not sufficient for Wnt3a secretion
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5a]-L-serine
CoA + [Wnt5a]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt5a]-L-serine
CoA + [Wnt5a]-O-(9Z)-hexadec-9-enoyl-L-serine
palmitoylation of Wnt-5a at Cys104
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine
CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [WntWg]-L-serine
CoA + [WntWg]-O-(9Z)-hexadec-9-enoyl-L-serine
the enzyme catalyzes the palmitoylation of Wnt Wingless or Wg. The enzyme binds the N-terminal 24-amino acid domain (residues 83-106) of Wg
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
the enzyme catalyzes posttranslational modification of Wnts with palmitoleic acid. PORCN is necessary and sufficient for Wnt acylation. PORCN intimately recognizes the local structure of Wnt around the site of acylation
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
there is an increase in the catalytic activity from hexanoyl-CoA to decanoyl-CoA, after which there is an abrupt drop for dodecanoyl-CoA. Upon further increase in fatty acyl chain length, the activity stays at approximately the same level until the unsaturated palmitoleoyl-CoA, which is the best substrate
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
the enzyme is an essential mediators of Wnt secretion and signaling
-
-
?
(9Z)-hexadec-9-enoyl-CoA + [Wnt]-L-serine
CoA + [Wnt]-O-(9Z)-hexadec-9-enoyl-L-serine
-
-
-
?
additional information
?
-
-
enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity
-
-
?
additional information
?
-
enzyme Porc functions on the N-terminal 24-amino acid domain (83-106) of Wg, binding of Porc with Wg is essential for its activity
-
-
?
additional information
?
-
-
recombinant c-Myc-tagged substrate Wg mutants T51A, S105A, S110A, and T416A
-
-
?
additional information
?
-
recombinant c-Myc-tagged substrate Wg mutants T51A, S105A, S110A, and T416A
-
-
?
additional information
?
-
-
vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification, localization of Wnt proteins in the chick neural tube, overview
-
-
?
additional information
?
-
PORCN splice variants differ globally in signaling activity
-
-
?
additional information
?
-
generation of a fusion protein that is palmitoylated in a PORCN-dependent manner, overview
-
-
?
additional information
?
-
no activity with palmitoylation-defective Wnt3aS209A mutant
-
-
?
additional information
?
-
-
no activity with palmitoylation-defective Wnt3aS209A mutant
-
-
?
additional information
?
-
vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification
-
-
?
additional information
?
-
-
vertebrate Wnt1 and Wnt3a possess at least one additional site for porcupine-mediated lipid-modification
-
-
?
additional information
?
-
no activity with palmitoylation-defective Wnt3aS209A mutant
-
-
?
