2.3.1.250: [Wnt protein] O-palmitoleoyl transferase
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For detailed information about [Wnt protein] O-palmitoleoyl transferase, go to the full flat file.
Reaction
Synonyms
CG6205, MG61, Mom-1, Mporc, mPorcD, PORC, PORCN, porcupine, PPN, protein-serine O-palmitoleoyltransferase porcupine, Xporc
ECTree
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Engineering
Engineering on EC 2.3.1.250 - [Wnt protein] O-palmitoleoyl transferase
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C187A
site-directed mutagenesis, increase in Wnt signaling activity of about 1.5fold upon expression of PORCNC187A compared to wild-type PORCN
C375X
site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 residue, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant
C381X
site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 residue, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant
C385X
site-directed mutagenesis, mutations introduced in PORCN at either Cys375, Cys381 or Cys385, all situated on a predicted luminal loop projecting from transmembrane domain VII that contains the catalytic His341 residue, have detrimental effects on Wnt3a palmitoylation and signaling that are comparable to that of the catalytically dead H341A mutant
H341A
additional information
H341A
site-directed mutagenesis, the mutation reduces Wnt signaling relative to wild-type PORCN
H341A
site-directed mutagenesis, catalytic active site mutant, Wnt signaling inactive mutant
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generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview
additional information
generation of three different deletion mutants of the enzyme lacking residues 1-34, 1-66, or 1-110 (DELTA34, DELTA66, and DELTA110), the mutants show affeced N-glycosylation of Wingless protein, because the mutant enzymes cannot bind to Wingless, overview
additional information
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rescue of Drosophila porc embryos by Mus musculus Mporc RNA injection
additional information
rescue of Drosophila porc embryos by Mus musculus Mporc RNA injection
additional information
identification of enzyme mutants with a 219-kb deletion in Xp11.23, heterozygous and mosaic mutations in gene PORCN, genotyping, overview
additional information
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identification of enzyme mutants with a 219-kb deletion in Xp11.23, heterozygous and mosaic mutations in gene PORCN, genotyping, overview
additional information
overexpression of HA-tagged human PORC to confirm the ability of hPORC RNAi constructs to knockdown hPORC expression
additional information
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overexpression of HA-tagged human PORC to confirm the ability of hPORC RNAi constructs to knockdown hPORC expression
additional information
the cysteine mutations might be detrimental to the enzyme's tertiary structure and its catalytic activity
additional information
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the cysteine mutations might be detrimental to the enzyme's tertiary structure and its catalytic activity
additional information
zinc finger nuclease-mediated PORCN gene deletion in HT1080 cells. Mutation or skipping of PORCN exon 9 creates cells null for PORCN catalytic activity
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PORCN is deleted by adenoviral Cre-GFP excision in immortalized MEF cells, PORCN null cells cannot activate WNT3A
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rescue of Drosophila melanogaster porc embryos by Mporc RNA injection
additional information
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rescue of Drosophila melanogaster porc embryos by Mporc RNA injection
additional information
the catalytically inactive porcupine is able to act as an inhibitor of endogenous porcupine, possibly via direct competition for substrate