2.3.1.247: 3-keto-5-aminohexanoate cleavage enzyme

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For detailed information about 3-keto-5-aminohexanoate cleavage enzyme, go to the full flat file.

Reaction

Synonyms

3-keto,5-aminohexanoate cleavage enzyme, FN1868, kce

ECTree

     2 Transferases

         2.3 Acyltransferases

             2.3.1 Transferring groups other than aminoacyl groups

                2.3.1.247 3-keto-5-aminohexanoate cleavage enzyme

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Results	in table
2	Activating Compound
408	AA Sequence
1	Cloned(Commentary)
1	Crystallization (Commentary)
11	General Information
5	Inhibitors
8	kcat/KM [mM/s]
9	KM Value [mM]
5	Metals/Ions
4	Molecular Weight [Da]
7	Natural Substrates/ Products (Substrates)
9	Organism
5	Pathway
3	pH Optimum
1	pH Range
5	Protein Variants
3	Purification (Commentary)
2	Reaction
1	Reaction Type
7	Reference
2	Source Tissue
4	Specific Activity [micromol/min/mg]
15	Substrates and Products (Substrate)
3	Subunits
8	Synonyms
1	Systematic Name
1	Temperature Optimum [°C]
8	Turnover Number [1/s]

Purification

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Purification on EC 2.3.1.247 - 3-keto-5-aminohexanoate cleavage enzyme

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

native enzyme 143fold by anion exchange chromatography

Fusobacterium nucleatum subsp. nucleatum

Q8RHX2

3378

native soluble enzyme 78fold by ammonium sulfate fractionation and anion exchange chromatography, followed by ultrafiltration and adsorption chromatography

Brevibacterium sp.

-

6044

recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, desalting gel filtration, anion exchange chromatography, and gel filtration

Fusobacterium nucleatum subsp. nucleatum

Q8RHX2

680885