2.3.1.241: Kdo2-lipid IVA acyltransferase

This is an abbreviated version!
For detailed information about Kdo2-lipid IVA acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.241

2.3.1.241

neisseria

meningitidis

meningococcal

lipopolysaccharide

bactericidal

penta-acylated

nomvs

hexa-acylated

endotoxic

serogrouping

poras

lipooligosaccharide

reactogenicity

monophosphoryl

underacylated

detergent-treated

2'-position

tetra-acylated

drug development

Reaction

(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose

(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose

Synonyms

(Kdo)2-lipid IVA lauroyltransferase, alpha-Kdo-(2->4)-alpha-(2->6)-lipid IVA lauroyltransferase, dodecanoyl-[acyl-carrier protein]:alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA O-dodecanoyltransferase, dodecanoyl-[acyl-carrier protein]:Kdo2-lipid IVA O-dodecanoyltransferase, ESA02293, htrB, HtrB1, Kdo2-lipid IVA lauroyltransferase, lauroyl-[acyl-carrier protein]:Kdo2-lipid IVA O-lauroyltransferase, lipid A biosynthesis lauroyltransferase, LpxL, LpxL1, LpxL2, lpxL_1, lpxL_2, PA0011, PP_1735

ECTree

2 Transferases

2.3 Acyltransferases

2.3.1 Transferring groups other than aminoacyl groups

2.3.1.241 Kdo2-lipid IVA acyltransferase

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Results	in table
2	Activating Compound
7114	AA Sequence
1	Application
5	Cloned(Commentary)
1	Expression
6	General Information
1	General Stability
2	Inhibitors
2	KM Value [mM]
1	Localization
1	Metals/Ions
2	Molecular Weight [Da]
2	Natural Substrates/ Products (Substrates)
13	Organism
12	Pathway
2	pH Optimum
5	Protein Variants
2	Purification (Commentary)
1	Reaction
10	Reference
2	Specific Activity [micromol/min/mg]
14	Substrates and Products (Substrate)
1	Subunits
27	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 2.3.1.241 - Kdo2-lipid IVA acyltransferase

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a dodecanoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose

(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-(dodecanoyloxy)tetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose + an [acyl-carrier protein]

5 entries

a dodecanoyl-[acyl-carrier protein] + Kdo2-lipid IVA

dodecanoyl-Kdo2-lipid IVA + an [acyl-carrier protein]

4 entries

a tetradecanoyl-[acyl-carrier protein] + Kdo2-lipid IVA

tetradecanoyl-Kdo2-lipid IVA + an [acyl-carrier protein]

3 entries

dodecanoyl-[acyl-carrier protein] + (3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->4)-(3-deoxy-alpha-D-manno-oct-2-ulopyranosylonate)-(2->6)-2-deoxy-2-[[(3R)-3-hydroxytetradecanoyl]amino]-3-O-[(3R)-3-hydroxytetradecanoyl]-4-O-phosphono-beta-D-glucopyranosyl-(1->6)-2-deoxy-3-O-[(3R)-3-hydroxytetradecanoyl]-2-[[(3R)-3-hydroxytetradecanoyl]amino]-1-O-phosphono-alpha-D-glucopyranose

Escherichia coli

P0ACV0

i.e. alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA. Kdo i.e. 3-deoxy-D-manno-octulosonic acid. The enzyme transfers laurate from lauroyl-acyl carrier protein to the 2'-R-3-hydroxymyristate moiety of the tetraacylated lipid A precursor Kdo2-lipid IVA. The enzyme catalyzes also a slow second acylation, generating Kdo2-(dilauroyl)-lipid IVA. The enzyme is capable of acylating lipid IVA in vitro. The specific activity with lipid IVA as the substrate is 6000fold lower than for Kdo2-lipid IVA. The presence of the Kdo disaccharide in the substrate accelerates laurate transfer by at least 3 orders of magnitude. The enzyme acylates the lipid A analogue 1-dephospho-Kdo2-lipid IVA with 10fold higher specific activity as compared to alpha-Kdo-(2->4)-alpha-Kdo-(2->6)-lipid IVA. Lauroyl-CoA can serve as an alternative acyl donor. Decanoyl-CoA, and to a lesser extent myristoyl-CoA, also function as acyl donors at low concentrations with inhibition of acylation at higher concentrations. Palmitoyl-CoA is inefficiently utilized as an acyl donor, but some acylation is observed. R-3-hydroxymyristoyl-[acyl-carrier protein] and synthetic R-3-hydroxylauroylmethylphosphopantetheine are poor acyl chain donors

726949

additional information

2 entries