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2.3.1.24: sphingosine N-acyltransferase

This is an abbreviated version!
For detailed information about sphingosine N-acyltransferase, go to the full flat file.

Word Map on EC 2.3.1.24

Reaction

an acyl-CoA
+
sphingosine
=
CoA
 +
a ceramide

Synonyms

(dihydro)ceramide synthase, acyl-CoA : sphingosine N-acyltramferase, acyl-CoA : sphingosine N-acyltransferase, acyltransferase, sphingosine, C16:0-CerS, CER2, Cer3, ceramide synthase, ceramide synthase 4, ceramide synthase 5, ceramide synthase 6, ceramide synthetase, CerS, CerS4, CerS45, CerS5, CerS6, CNAG_02086, CNAG_02087, CNAG_06717, DLag1, Drosophila longevitiy assurance gene-1 homologue, fatty acyl-CoA:sphingosine acyltransferase, LAC1, LAG1, LAG1 longevity assurance homolog 2, LAG1 longevity assurance homolog 5, LAG12, LASS5, Lass6, LOH2, longevity assurance homolog 5, longevity assurance homolog 6, longevity assurance homologue 5, longevity assurance homologue 6, More, schlank, SpAT, sphinganine N-acyl transferase synthase, sphinganine N-acyltransferase, sphingosine acyltransferase, sphingosine N-acyltransferase, sphingosine/sphinganine acyltransferase, TcCerS, TcCERS1, TRAM homolog 4, translocating chain-associating membrane protein homolog 4, trh1-like, trh4

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.24 sphingosine N-acyltransferase

Cloned

Cloned on EC 2.3.1.24 - sphingosine N-acyltransferase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells
DNA and amino acid sequence determination and analysis, sequence comparisons, phylogenetic tree, recombinant expression of HA-tagged enzyme in HEK-293T cells, and recombinant expression of C-terminally GFP-tagged Lass6
expression in HEK-293T cell
expression of wild-type and mutant enzymes in Escherichia coli strain BJ5183, and transient expression in HEK293A cells, HT29 cells, and SW620 cells from an adenovirus vector
gene CERS4, isozyme expression analysis
gene CERS4, quantitative real-time PCR enzyme expression analysis, transient overexpression of HA-tagged CerS4 in INS-1 cells increasing the levels of ceramides C18:0 and C22:0 by 20%, but it has no effect on the accumulation of ceramides C16:0 and C24:0
gene CERS4, recombinant overexpression in HEK cells
gene CERS5, quantitative real-time PCR enzyme expression analysis
gene CERS5, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis
gene CERS5, recombinant expression of HA-tagged wild-type enzyme, and point mutants as well as truncated CerS5DELTAC332-392 mutant and chimeric mutant enzyme in HEK-293 cells, recombinant expression of FLAG-tagged CerS5 in HEK-293 cells
gene CERS5, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS5 overexpression significantly increases apoptosis in a time- and dose-dependent manner compared to empty vector-transfected controls. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells
gene CERS5, recombinant overexpression in HEK cells, FTY720 inhibits CerS1 activity to a greater extent than CerS5 in the overexpressing cells
gene CERS5, stable recombinant ectopic overexpression of N-terminally FLAG3-tagged CerS5 in HEK-293 or HeLa cells, the level of FLAG-tagged CerS5 remains constant after exposure to cisplatin, doxorubicin, or UV light
gene Cers5, transient recombinant expression of His-tagged LASS5 in MLE cells
gene CERS6, isozyme expression analysis
gene CERS6, quantitative real-time PCR enzyme expression analysis
gene CERS6, recombinant expression in HEK-293T cells, quantitative real-time quantitative PCR enzyme expression analysis
gene CERS6, recombinant expression of N-terminally FLAG-tagged CerS6 and EGFP-tagged CerS6 in SW-480 cells and localization in the perinuclear region. When cells transfected with pCerS6-IRES2-EGFP are treated with TRAIL, GFP positive cells appear to be undergoing apoptosis. Cells stably transfected with CerS6 but not with the empty plasmid acquire a TRAIL sensitive phenotype
gene CERS6, recombinant FLAG-tagged isozyme overexpression in HeLa cells, quantitative real-time PCR enzyme expression analysis, CerS6 overexpression yields no significant differences in IR-induced apoptosis compared to empty vector-transfected cells. CerS2, CerS5, and CerS6 form heterocomplexes in transformed HeLa cells
gene LAG2, recombinant expression of codon-optimized LAG2 as N-terminally FLAG-tagged enzyme in Saccharomyces cerevisiae membranes
gene schlank, located on X-chromosome
-
MLE cells transiently transfected with His-tagged LASS5 plasmid