2.3.1.217: curcumin synthase

This is an abbreviated version!
For detailed information about curcumin synthase, go to the full flat file.

= 2 CoA +

curcumin synthase, curcumin synthase 1, curcumin synthase 2, CURS, CURS1, CURS2, CURS3, OsCUS, ZoCURS

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Results	in table
8	AA Sequence
9	Application
9	Cloned(Commentary)
1	Crystallization (Commentary)
2	Expression
8	General Information
9	kcat/KM [mM/s]
11	KM Value [mM]
2	Molecular Weight [Da]
6	Natural Substrates/ Products (Substrates)
15	Organism
2	Pathway
3	pH Optimum
4	Protein Variants
2	Purification (Commentary)
1	Reaction
1	Reaction Type
15	Reference
4	Source Tissue
19	Substrates and Products (Substrate)
20	Synonyms
1	Systematic Name
3	Temperature Optimum [°C]
10	Turnover Number [1/s]

Engineering on EC 2.3.1.217 - curcumin synthase

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G211F

site-directed mutagenesis, the mutant shows highly reduced 2-oxoacid condensation activity compared to the wild-type enzyme

H303A

site-directed mutagenesis, active site residue mutant, the mutant shows reduced 2-oxoacid condensation activity compared to the wild-type enzyme

H303Q

site-directed mutagenesis, active site residue mutant, the mutant shows reduced 2-oxoacid condensation activity compared to the wild-type enzyme

additional information

a curcuminoid producing unnatural fusion protein diketide-CoA synthase:curcumin synthase is constructed