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2.3.1.216: 5,7-dihydroxy-2-methylchromone synthase

This is an abbreviated version!
For detailed information about 5,7-dihydroxy-2-methylchromone synthase, go to the full flat file.

Word Map on EC 2.3.1.216

Reaction

5 malonyl-CoA = 5 CoA +

5,7-dihydroxy-2-methyl-4H-chromen-4-one
+ 5 CO2 +
H2O

Synonyms

PCS, pentaketide chromone synthase

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.216 5,7-dihydroxy-2-methylchromone synthase

Crystallization

Crystallization on EC 2.3.1.216 - 5,7-dihydroxy-2-methylchromone synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystal structure analysis, molecular replacement
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purified detagged recombinant pentaketide chromone synthase, hanging drop vapour diffusion method, mixing of 500 nl of 10 mg/ml protein in 20 mM HEPES-NaOH, pH 7.0, containing 100 mM NaCl, and 2 mM DTT, with 500 nl reservoir solution containing 100 mM Tris-HCl, pH 8.5, 14% w/v PEG 8000, and 350 mM KF, 20°C, 2 days, X-ray diffraction structure determination and analysis at 1.6 A resolution
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