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2.3.1.212: benzalacetone synthase

This is an abbreviated version!
For detailed information about benzalacetone synthase, go to the full flat file.

Word Map on EC 2.3.1.212

Reaction

4-coumaroyl-CoA
+
malonyl-CoA
 +
H2O
= 2 CoA +
4-hydroxybenzalacetone
 + 2 CO2

Synonyms

bAS, HsPKS3, PKS1, RinPKS1, RiPKS4, RpBAS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.212 benzalacetone synthase

Crystallization

Crystallization on EC 2.3.1.212 - benzalacetone synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
complex between PKS1 and benzalacetone, to 2.0 A resolution, orthorhombic space group P212121, with unit-cell parameters a: 80.23, b: 81.01, c: 122.89 A
-
purified enzyme, sitting drop vapour diffusion method, mixing of 500 nl of protein solution containing 20 mg/ml protein in 20 mM HEPES-NaOH, pH 7.5, 100 mM NaCl, and 2 mM DTT, with 500 nl of reservoir solution containing 100 mM MES-NaOH, pH 6.5, 15% w/v PEG 8000, and 200 mM potassium thiocyanate, and equilibration against 0.1 ml of reservoir solution, 3 days, cryoprotection by 20% v/v glycerol in reservoir solution, X-ray diffraction structure determination and analysis at 1.8 A resolution