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2.3.1.211: bisdemethoxycurcumin synthase

This is an abbreviated version!
For detailed information about bisdemethoxycurcumin synthase, go to the full flat file.

Word Map on EC 2.3.1.211

Reaction

2 4-coumaroyl-CoA +

malonyl-CoA
+
H2O
= 3 CoA +
bisdemethoxycurcumin
+ 2 CO2

Synonyms

ClPKS10, curcumin synthase, curcuminoid synthase, CUS, Os07g0271500, OsCUS

ECTree

     2 Transferases
         2.3 Acyltransferases
             2.3.1 Transferring groups other than aminoacyl groups
                2.3.1.211 bisdemethoxycurcumin synthase

Crystallization

Crystallization on EC 2.3.1.211 - bisdemethoxycurcumin synthase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, optimization of the crystallization conditions, mixing 0.0005 ml of protein solution, containing 10 mg/ml in 20 mM HEPES-NaOH, pH 7.0, 100 mM NaCl, and 2 mM DTT, with an equal volume of reservoir solution, containing 100 mM HEPES-NaOH, pH 7.8, 1995 mM ammonium sulfate, 3% dimethylsulfoxide, and 2 mM CoA, and equilibration against 0.1 ml reservoir solution, 20°C, cryoprotection in 100 mM HEPES-NaOH pH 7.8, 1330 mM ammonium sulfate, 2 mM CoA, 12% v/v glycerol and 14% w/v PEG 400, X-ray diffraction structure determination and analysis st 2.5 A resolution
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