2.3.1.211: bisdemethoxycurcumin synthase
This is an abbreviated version!
For detailed information about bisdemethoxycurcumin synthase, go to the full flat file.
Word Map on EC 2.3.1.211
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2.3.1.211
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curcuminoids
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oryza
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polyketide
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sativa
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synthesis
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turmeric
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starter
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curcuma
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longa
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thioester
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diarylheptanoids
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gingerol
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4-coumaroyl-coas
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phenylpropanoids
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rhizome
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hepatoprotective
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erythrorhizon
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cinnamic
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diketide
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one-pot
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shoot
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ammonia-lyase
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4-coumarate
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plant-specific
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lithospermum
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4-coumarate:coa
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analysis
- 2.3.1.211
-
curcuminoids
- oryza
- polyketide
- sativa
- synthesis
- turmeric
-
starter
-
curcuma
- longa
- thioester
-
diarylheptanoids
- gingerol
- 4-coumaroyl-coas
-
phenylpropanoids
- rhizome
-
hepatoprotective
- erythrorhizon
-
cinnamic
-
diketide
-
one-pot
- shoot
-
ammonia-lyase
- 4-coumarate
-
plant-specific
-
lithospermum
-
4-coumarate:coa
- analysis
Reaction
2 4-coumaroyl-CoA + + = 3 CoA + + 2 CO2
Synonyms
ClPKS10, curcumin synthase, curcuminoid synthase, CUS, Os07g0271500, OsCUS
ECTree
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Crystallization
Crystallization on EC 2.3.1.211 - bisdemethoxycurcumin synthase
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purified recombinant His6-tagged enzyme, sitting drop vapour diffusion method, optimization of the crystallization conditions, mixing 0.0005 ml of protein solution, containing 10 mg/ml in 20 mM HEPES-NaOH, pH 7.0, 100 mM NaCl, and 2 mM DTT, with an equal volume of reservoir solution, containing 100 mM HEPES-NaOH, pH 7.8, 1995 mM ammonium sulfate, 3% dimethylsulfoxide, and 2 mM CoA, and equilibration against 0.1 ml reservoir solution, 20°C, cryoprotection in 100 mM HEPES-NaOH pH 7.8, 1330 mM ammonium sulfate, 2 mM CoA, 12% v/v glycerol and 14% w/v PEG 400, X-ray diffraction structure determination and analysis st 2.5 A resolution
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